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Therapeutic Effects of Different Animal Bile Powders on Lipid Metabolism Disorders and Their Composition Analysis

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Abstract

Objective

To compare the therapeutic effect of different animal bile powders on lipid metabolism disorders induced by high-fat diet in rats, and analyze the bioactive components of each animal bile powder.

Methods

Sixty Sprague-Dawley rats were randomly divided into 6 groups (n=10): normal diet control group, high-fat diet model group, high-fat diet groups orally treated with bear, pig, cow and chicken bile powders, respectively. Serum biochemical markers from the abdominal aorta in each group were analyzed. Changes in the body weight and liver weight were recorded. Pathohistological changes in the livers were examined. High performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was used to determine the composition of bioactive components in each animal bile powder.

Results

Treatment with different types of animal bile powders had different inhibitory effects on high-fat diet-induced increase of body weight and/or liver weight in rats, most notably in bear and pig bile powders (P<0.05). High-fat diet induced lipid metabolism disorder in rats, which could be reversed by treatment with all kinds of bile powders. Bear bile and chicken bile showed the most potent therapeutic effect against lipid metabolism disorder. Cow and bear bile effectively alleviated high-fat diet induced liver enlargement and discoloration, hepatocyte swelling, infiltration of inflammatory cells and formation of lipid vacuoles. Bioactive component analysis revealed that there were significant differences in the relative content of taurocholic acid, taurodeoxycholic acid and ursodeoxycholic acid among different types of animal bile. Interestingly, a unique component with molecular weight of 496.2738 Da, whose function has not yet been reported, was identified only in bear bile powder.

Conclusions

Different animal bile powders had varying therapeutic effect against lipid metabolism disorders induced by high-fat diet, and bear bile powder demonstrated the most effective benefits. Bioactive compositions were different in different types of animal bile with a novel compound identified only in bear bile powder.

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Authors and Affiliations

  1. Academy of Integrative Medicine Cardiovascular Research Institute, Fuzhou, 350122, China

    Da-xin Chen, Jian-feng Chu, Shan Lin, Ling Zhang, Hong-wei Chen, Zhi-wei Sun, Qiao-yan Cai, Li-li Wang & Jun Peng

  2. Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China

    Da-xin Chen, Jian-feng Chu, Shan Lin, Ling Zhang, Hong-wei Chen, Zhi-wei Sun, Qiao-yan Cai, Li-li Wang & Jun Peng

  3. Chen Ke-ji Academic Thought Heritage Studio, Fuzhou, 350122, China

    Da-xin Chen, Jian-feng Chu, Shan Lin, Hong-wei Chen, Qiao-yan Cai & Jun Peng

  4. Fujian Guizhentang Pharmaceutical Co., Ltd., Huian, Fujian Province, 362142, China

    Jian-feng Xu

Authors
  1. Da-xin Chen
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  2. Jian-feng Chu
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  3. Shan Lin
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  4. Ling Zhang
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  5. Hong-wei Chen
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  6. Zhi-wei Sun
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  7. Jian-feng Xu
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  8. Qiao-yan Cai
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  9. Li-li Wang
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  10. Jun Peng
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Contributions

Chen DX conceived and designed the experiments, analyzed the data and wrote the manuscript; Chu JF, LIN S and Chen HW performed the animal experiments; Zhang L and Cai QY participate in the pharmacology experiments; Chen DX, Wang LL performed the composition analysis, Sun ZW and Xu JF contributed reagents; Peng J revisied the manuscript critically for important intellectual content, provided scientific advice and final approval of the version to be published.

Corresponding author

Correspondence to Jun Peng.

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Conflicts of Interest

The authors declare no financial or commercial conflict of interest.

Supported by the Developmental Fund of Chen Keji Integrative Medicine (No. CKJ2013009) and Project-Oriented Collaboration with Fujian Guizhentang Pharmaceutical Co., Ltd. (No. 701141002)

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Chen, Dx., Chu, Jf., Lin, S. et al. Therapeutic Effects of Different Animal Bile Powders on Lipid Metabolism Disorders and Their Composition Analysis. Chin. J. Integr. Med. 28, 918–923 (2022). https://doi.org/10.1007/s11655-021-3441-3

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  • DOI: https://doi.org/10.1007/s11655-021-3441-3

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