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Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells

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Abstract

Objective

To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods

Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted. The nitric oxide (NO) production was measured using the Griess assay. Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay. The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis. Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.

Results

Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05). Moreover, BNH inhibited protein levels of iNOS and COX-2 (P<0.01). Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).

Conclusion

The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.

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Authors and Affiliations

  1. Chuncheon Center, Korea Basic Science Institute, Chuncheon, 24341, Republic of Korea

    Su-Hyeon Cho, Song Rae Kim, Myeong Seon Jeong, Miri Choi, SeonJu Park & Kil-Nam Kim

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  1. Su-Hyeon Cho
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  2. Song Rae Kim
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  3. Myeong Seon Jeong
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  4. Miri Choi
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  5. SeonJu Park
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  6. Kil-Nam Kim
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Contributions

K.N.K. supervised and designed the study. S.H.C., S.R.K., M.S.J., M.C., and S.J.P performed experimental analysis. S.H.C. and K.N.K wrote the manuscript.

Corresponding author

Correspondence to Kil-Nam Kim.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Supported by the Korea Basic Science Institute (No. C38915 and C030360)

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Cho, SH., Kim, S.R., Jeong, M.S. et al. Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells. Chin. J. Integr. Med. 27, 273–279 (2021). https://doi.org/10.1007/s11655-021-3330-9

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  • DOI: https://doi.org/10.1007/s11655-021-3330-9

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