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Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

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Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the effects and underlying mechanisms of Panax quinquefolium saponin (PQS) on energy deficiency in hypoxia-reperfusion (H/R) induced cardiomyocytes.

Methods

The H/R injury involved hypoxia for 3 h and then reperfusion for 2 h. Cardiomyocytes recruited from neonatal rat ventricular myocytes (NRVMs) were randomly divided into control, H/R, H/R+compound C (C.C), H/R+PQS, and H/R+C. C+PQS groups. BrdU assay, lactase dehydrogenase (LDH) leakage and early apoptosis rate were evaluated to assess cell damages. Contents of high energy phosphate compounds were conducted to detect the energy production. Protein expression levels of adenosine monophosphate-activated protein kinase a (AMPKα), glucose transporter 4 (GLUT4), phosphate fructose kinase 2 (PFK2), fatty acid translocase/cluster of differentiation 36 (FAT/CD36), and acetyl CoA carboxylase 2 (ACC2) in the regulatory pathways were measured by Western blotting. Immunofluorescence staining of GLUT4 and FAT/CD36 was used to observe the mobilization of metabolic transporters.

Results

PQS (50 mg/L) pretreatment significantly alleviated H/R-induced inhibition of NRVMs viability, up-regulation of LDH leakage, acceleration of early apoptosis, and reduction of energy production (P<0.05). Compared with the H/R group, up-regulated expression of AMPKα, GLUT4, PFK2, FAT/CD36 and ACC2 were observed, and more GLUT4 and FAT/CD36 expressions were detected on the membrane in the H/R+PQS group (P<0.05). These effects of PQS on H/R-induced NRVMs were eliminated in the H/R+C.C+PQS group (P<0.05).

Conclusion

PQS has prominent advantages in protecting NRVMs from H/R-induced cell damages and energy metabolic disorders, by activation of AMPKα-mediated GLUT4-PFK2 and FAT/CD36-ACC2 pathways.

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Authors and Affiliations

  1. Department of Traditional Chinese Medicine, China-Japan Friendship Hospital, Beijing, 100029, China

    Yong-hui Yu

  2. Graduate Shcool, Beijing University of Chinese Medicine, Beijing, 100029, China

    Pei Zhang

  3. Department of Cardiovascular Disease, Mongolian Hospital of Traditional Chinese Medicine, Wuhai, Inner Mongolia Autonomous Region, 016000, China

    Pei Zhang

  4. Center for Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Cheng-long Wang, Jian-gang Liu, Peng Li & Da-wu Zhang

Authors
  1. Yong-hui Yu
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  2. Pei Zhang
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  3. Cheng-long Wang
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  4. Jian-gang Liu
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  5. Peng Li
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  6. Da-wu Zhang
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Contributions

Wang CL, Liu JG and Zhang DW conceived and designed the study. Li P guided the extraction and culture of NRVMs. Zhang P, Yu YH and Liu JG performed the experiments. Yu YH and Zhang P analyzed the data and wrote the manuscript. All authors contribute to the preparation of the manuscript.

Corresponding author

Correspondence to Cheng-long Wang.

Ethics declarations

We declared no conflicts of interest in this work.

Additional information

Supported by the National Natural Science Foundation of China (No. 81273934 and No. 81874410)

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Cite this article

Yu, Yh., Zhang, P., Wang, Cl. et al. Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes. Chin. J. Integr. Med. 27, 613–620 (2021). https://doi.org/10.1007/s11655-020-3194-4

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  • DOI: https://doi.org/10.1007/s11655-020-3194-4

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