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Edible Bird’s Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increaing Bone Estrogen-Receptor Expression

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Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird’s nest (EBN) in ovariectomized rats.

Methods

Forty-two female Sprage-Dawley rats were randomized into 7 groups (6 in each group). The ovariectomized (OVX) and OVX + 6%, 3%, and 1.5% EBN and OVX +estrogen groups were given standard rat chow alone, standard rat chow +6%, 3%, and 1.5% EBN, or standard rat chow +estrogen therapy (0.2mg/kg per day), respectively. The sham-operation group was surgically opened without removing the ovaries. The control group did not have any surgical intervention. After 12 weeks of intervention, blood samples were taken for serum estrogen, osteocalcin, and osteoprotegerin, as well as the measurement of magnesium, calcium abd zinc concentrations. While femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique, then collected for histology and estrogen receptor (ER) immunohistochemistry.

Results

Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain, while estrogen and EBN supplementation attenuated these changes. Ovariectomy also reduced bone ER expression and density, and the production of osteopcalcin and osteorotegerin, which are important pro-osteoplastic hormones that promote bone mineraliztion and density. Conversely, estrogen and EBN increased serum estrogen levels leading to increased bone ER expression, pro-osteoplastic hormone production and bone density (all P<0.05).

Conclusion

EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.

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Author information

Authors and Affiliations

  1. Department of Pathology, Chengde Medical University, Chengde, Hebei Province, 067000, China

    Zhi-ping Hou, Shi-ying Tang, Hai-ru Ji, Yu-hong Li, Xian-ling Dong & Meng-nan Du

  2. Department of Gastroenterology, The Affiliated Hospital of Chengde Medical University, Chengde, Hebei Province, 067000, China

    Pei-yuan He

  3. Department of Pathology, The Affiliated Hospital of Hebei University, Baoding, Hebei Province, 071000, China

    Meng-nan Du

  4. Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia

    Ismail Maznah

  5. Department of Gastroscopy, Kuancheng Manchu Autonomous County Hospital, Chengde, Hebei Province, 067000, China

    Wen-jing He

Authors
  1. Zhi-ping Hou
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  2. Shi-ying Tang
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  3. Hai-ru Ji
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  4. Pei-yuan He
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  5. Yu-hong Li
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  6. Xian-ling Dong
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  7. Meng-nan Du
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  8. Ismail Maznah
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  9. Wen-jing He
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Contributions

Hou ZP conceived the idea for this study and carried out the experiment, and together with He PY and Ismail M drafted the mamuscript. Ji HR and Du MN helped cultured rats. Tang SY and Li YH participated in its design and coordination. Dong XL and He WJ helped to calculate bone density. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Zhi-ping Hou.

Ethics declarations

The authors declare that they have no competing interests.

Additional information

Supported by Grants from Ministry of Science, Technology and Innovation, E-science Fund (No. 5450666), Malaysia; the Health and Family Planning Commission of Hebei (No. 20160315); the Administration of Traditional Chinese Medicine of Hebei (No. 2017196); the Key Discipline Construction Project of Hebei Provincial Universities [No. JiJiaoGao-2013-(4)-2012-37]; and Start up Fund for High Level Talents of Chengde Medical University (No. 201704)

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11655_2019_3209_MOESM1_ESM.pdf

Edible Bird’s Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increasing BoneEstrogen-Receptor Expression

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Hou, Zp., Tang, Sy., Ji, Hr. et al. Edible Bird’s Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increaing Bone Estrogen-Receptor Expression. Chin. J. Integr. Med. 27, 280–285 (2021). https://doi.org/10.1007/s11655-019-3209-1

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  • DOI: https://doi.org/10.1007/s11655-019-3209-1

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