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Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells

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Abstract

Objective

To examinie the synergistic effects of Banxia Xiexin Decoction (半夏泻心汤, Known as Banhasasim-tang in Korean) extract (BXDE) on cisplatin-induced cytotoxicity in the A549 human lung cancer cell lines.

Methods

A549 cells were treated with varying concentrations (50–200 μg/mL) of cisplatin and BXDE alone or in combination for 96 h. We used 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay and flow cytometry to analyze cell viability and apoptosis, respectively.

Results

The exposure of cells to cisplatin and BXDE alone or in combination decreased cell viability dose- and time-dependently (P<0.05), which was found to be mediated by the apoptotic pathway as confirmed by the increase in the annexin V+/propidium iodide- stained cell population and a ladder pattern of discontinuous DNA fragments. Furthermore, the apoptosis was inhibited by the pan-caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (z-VAD-FMK).

Conclusions

BXDE significantly potentiated apoptotic effects of cisplatin in A549 cells. Moreover, apoptosis induced by BXDE might be the pivotal mechanism mediating its chemopreventative action against cancer.

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Authors and Affiliations

  1. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea

    Ha-Rim Kim, Mi-Seong Kim, Hong-Seob So, Young-Rae Lee & Kang-Beom Kwon

  2. Department of Herbology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk, 54538, South Korea

    Guem-San Lee

  3. Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan Jeonbuk, 54538, South Korea

    Do-Gon Ryu & Kang-Beom Kwon

  4. Institute of Customized Physical Therapy, Gwanju, 62279, South Korea

    Hyoung-Chul Moon

  5. Department of Oral Biochemistry, Wonkwang University School of Dentistry, Iksan, Jeonbuk, 54538, South Korea

    Young-Rae Lee

  6. Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea

    Sei-Hoon Yang

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  1. Ha-Rim Kim
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  2. Guem-San Lee
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  9. Kang-Beom Kwon
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Correspondence to Kang-Beom Kwon.

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Kim, HR., Lee, GS., Kim, MS. et al. Effects of Banxia Xiexin Decoction (半夏泻心汤) on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Cells. Chin. J. Integr. Med. 24, 436–441 (2018). https://doi.org/10.1007/s11655-017-2922-x

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  • DOI: https://doi.org/10.1007/s11655-017-2922-x

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