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Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats

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Abstract

Objective

To investigate the impact of dampness-heat (DH) on the development of mammary tumors in 7,12-dimethylbenz(a)anthracene (DMBA)-induced rats.

Methods

Forty rats were randomly divided into 3 groups in a randomized block design, including the control group (n=13), DMBA group (n=14), and DMBA plus DH group (n=13). Rats in the DMBA group and DMBA plus DH group were intragastrically administrated with DMBA (100 mg/kg) for twice, once per week, while rats in the control group were treated with equivalent volumes of sesame oil. After DMBA administration, rats in the DMBA plus DH group were exposed to a simulated climate chamber with ambient temperature (33.0±0.5°C) and humidity (90%±5%) for 8 weeks, 8 h per day. The body weight, time of tumor formation, and number of tumors were measured weekly to calculate tumor incidence, average latency period, average number of tumors, and average tumor weight. At the end of the experiment, the levels of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinases 1 (TIMP-1) in serum, and the contents of tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β in serum and tumor tissue were measured, respectively. Some tumor tissues were processed for hematoxylin-eosin staining to determine the histopathological changes.

Results

Compared with DMBA, DMBA plus DH significantly increased the average number of tumors, average tumor weight, levels of serum MMP-9, TIMP-1, TNF-α and IL-1β, and contents of tumor tissue TNF-α and IL-1β (P<0.05 or P<0.01).

Conclusion

DH could accelerate the development of mammary tumors through increasing the expressions of MMP-9, TIMP-1, TNF-α and IL-1β in DMBA-induced rats.

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Authors and Affiliations

  1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China

    Wei Dong, Wei-feng Zhu & Hong-ning Liu

  2. Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, 330013, China

    Xi-lan Tang

  3. Research Center for Differentiation and Development of Basic Theory of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China

    Guang-bin Shang & Guo-liang Xu

Authors
  1. Wei Dong
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  2. Xi-lan Tang
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  3. Guang-bin Shang
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  4. Guo-liang Xu
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  5. Wei-feng Zhu
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  6. Hong-ning Liu
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Corresponding author

Correspondence to Hong-ning Liu.

Additional information

Supported by the National Natural Science Foundation of China (No. 81260523)

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Dong, W., Tang, Xl., Shang, Gb. et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats. Chin. J. Integr. Med. 24, 758–762 (2018). https://doi.org/10.1007/s11655-017-2821-1

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  • DOI: https://doi.org/10.1007/s11655-017-2821-1

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