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Inhibition of proliferation of prostate cancer cell line DU-145 in vitro and in vivo using Salvia miltiorrhiza Bunge.

  • Woong Jin Bae
  • Jin Bong Choi
  • Kang Sup Kim
  • U. Syn Ha
  • Sung Hoo Hong
  • Ji Youl Lee
  • Tae-Kon Hwang
  • Sung Yeoun Hwang
  • Zhi-ping Wang
  • Sae Woong KimEmail author
Original Article

Abstract

Objective

To investigate the antiproliferative activity of Salvia miltiorrhiza Bunge. (SM) on the castration-resistant prostate cancer (CRPC) cell line DU-145, in vitro and in vivo.

Methods

Prostate cancer cell line (DU-145) and normal prostate cell line (RWPE-1) were treated with SM at different concentrations (3.125, 12.5, 25 and 50 μg/mL) to investigate the antiproliferative effects. DNA laddering analysis was performed to investigate the apoptosis of DU-145 cells. Molecular mechanism was investigated by Western blot analysis of p53, Bcl-2, prostate specific antigen (PSA), and androgen receptor (AR). Six-week-old male BALB/c nude mice were randomly divided into normal control group (n=101) and treated group (n=101) which administered 500 mg/kg SM for 2 weeks. Tumor volumes were measured.

Results

Treatment with SM resulted in a dose-dependent decrease in cell number of DU-145 cells in comparison with RWPE-1. DNA laddering analysis indicated the apoptosis of DU-145 cells. Treatment with SM increased the expression of p53 and reduced the expression of Bcl-2 proteins. The levels of PSA were considerably reduced in SM-treated group compared to the controls, and a decrease in AR expression was observed when cells were treated with SM in the same pattern as a reduction in PSA. In the tumour xenograft study, SM given once a day for 2 weeks significantly inhibited tumour growth.

Conclusion

SM might contribute to the anticancer actions such as induction of apoptosis and inhibition of proliferation of prostate cancer cells.

Keywords

apoptosis prostate cancer Salvia miltiorrhiza Bunge. 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Woong Jin Bae
    • 1
    • 2
  • Jin Bong Choi
    • 1
  • Kang Sup Kim
    • 1
  • U. Syn Ha
    • 1
  • Sung Hoo Hong
    • 1
  • Ji Youl Lee
    • 1
  • Tae-Kon Hwang
    • 1
  • Sung Yeoun Hwang
    • 3
  • Zhi-ping Wang
    • 4
  • Sae Woong Kim
    • 1
    • 2
    Email author
  1. 1.Department of UrologyThe Catholic University of Korea, College of MedicineSeoulRepublic of Korea
  2. 2.Catholic Integrative Medicine Research InstituteCollege of Medicine, The Catholic University of KoreaSeoulRepublic of Korea
  3. 3.Korea Bio-Medical Science InstituteSeoulRepublic of Korea
  4. 4.Department of UrologySecond Hospital of Lanzhou UniversityLanzhouChina

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