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Effect of Modified Wuzi Yanzong Pill (五子衍宗丸) on Tip60-Mediated Apoptosis in Testis of Male Rats after Microwave Radiation

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Abstract

Objective

To investigate the effect of a modified Wuzi Yanzong Pill (五子衍宗丸, WZYZP) on the male rats’ testis after microwave radiation, as well as its potential mechanism.

Methods

Forty-five male rats were randomly assigned to three groups: the control group, the radiation group, and the WZYZP group. The rats in the radiation group and WZYZP group were exposed to microwave radiation for 15 min once, while the rats in the control group were not exposed to any radiation. The rats in the WZYZP group were given a modified of WZYZP by gavage daily for 7 days. Apoptosis in the testis was evaluated using terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) assay. Histopathological alterations of the testis were observed by haematoxylin-eosin (HE) staining. Tat-interactive protein, 60kD (Tip60) and p53 expressions were determined by Western blotting.

Results

The apoptosis index (AI) in the radiation group was higher than that of the WZYZP group and control group on day 1 (D1), day 7 (D7) day 14 (D14) after radiation (P<0.05). The seminiferous tubules were of normal morphology in the control group. In the radiation group, the partial seminiferous tubules were collapsed, basement membranes of the seminiferous epithelia became detached. WZYZP could restore the morphological changes. There was no expression of Tip60 among the three groups on D7 and D14. The expression of p53 was higher in the radiation group than in the control group (P<0.05). WZYZP could down-regulate the rising p53 induced by radiation on D7 and D14 (P<0.05).

Conclusion

A modified WZYZP may affect germ cells, and its protective effects may partly result from its ability to intervene in Tip60 mediated apoptosis.

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Authors and Affiliations

  1. Department of Andrology, Air Force General Hospital, PLA, Beijing, 100142, China

    Hai-xiang Hu, Jing Sun, Shao-qiang Xu, Li-zhao Wei, Shao-bo Luo, Chuan-yun Shen, Qi-long Zhang & Ya-lan Xie

  2. Department of Chinese Medicine, Fangzhuang Community Health Service Center, Beijing, 100078, China

    Ya-jing Gao

  3. Department of Radiology, Air Force General Hospital, PLA, Beijing, 100142, China

    Hong Fang

  4. Department of Traditional Chinese Medicine, Air Force General Hospital, PLA, Beijing, 100142, China

    Jing Dong

Authors
  1. Hai-xiang Hu
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  2. Jing Sun
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  3. Ya-jing Gao
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  4. Hong Fang
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  5. Shao-qiang Xu
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  6. Jing Dong
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  7. Li-zhao Wei
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  8. Shao-bo Luo
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  9. Chuan-yun Shen
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  10. Qi-long Zhang
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  11. Ya-lan Xie
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Corresponding author

Correspondence to Hai-xiang Hu.

Additional information

Supported by the National Natural Science Foundation of China (No. 30972991): Research Projects of the PLA General Logistics Department, China (No. BWS12J077 and No. BKJ16J010)

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Hu, Hx., Sun, J., Gao, Yj. et al. Effect of Modified Wuzi Yanzong Pill (五子衍宗丸) on Tip60-Mediated Apoptosis in Testis of Male Rats after Microwave Radiation. Chin. J. Integr. Med. 25, 342–347 (2019). https://doi.org/10.1007/s11655-017-2425-9

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  • DOI: https://doi.org/10.1007/s11655-017-2425-9

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