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Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy

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Abstract

Objective

To evaluate the renal protective effect of Tangshenkang Granule (糖肾康颗粒) in a rat model of diabetic nephropathy (DN).

Methods

Forty male Sprague-Dawley rats were randomly divided into control, DN, Tangshenkang and benazepril groups. DN model was established in the rats of DN, Tangshenkang and benazepril groups. Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks, respectively. Urinary albumin and creatinine were detected. The albumin/creatinine (ACR) was calculated in addition to 24 h urinary protein (24-h UPr), serum creatinine (Scr), blood urea nitrogen (BUN), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and creatinine clearance rate (Ccr). Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining. The average glomerular diameter (DG), average glomerular (AG) and mesangial areas (AM) were measured. The thickness of glomerular basement membrane (TGBM) was detected using transmission electron microscope.

Results

Compared with rats in the control group, rats in the DN group showed significantly decreased body weight, increased hypertrophy index, 24-h urinary volume, 24-h UPr, ACR, Scr, BUN, Ccr, blood lipids as well as renal pathological indices including DG, AG, AM, AM/AG and TGBM (P <0.05). Compared with the DN group, the weights of rats in the Tangshenkang and benazepril groups were significantly increased, and the renal hypertrophy indices were significantly decreased (P <0.05). The 24-h urinary volumes, ACR, 24-h UPr, Scr, BUN, Ccr, LDL, DG, AG, AM and TGBM were obviously decreased (P <0.05). Compared with the benazepril group, the Tangshenkang group showed significantly decreased levels of ACR, 24-h UPr, AG and AM (P <0.05).

Conclusions

Tangshenkang Granule decreased the urinary protein, attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats, and prevented further injury induced by diabetic nephropathy.

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References

  1. Zhang LX, Wang HY. Study on the trends and countermeasures of diabetic nephropathy in China from the perspective of epidemiology. Chin J Intern Med (Chin) 2010;49:804–805.

    Google Scholar 

  2. Lv Y, Wang YP, Cao EZ, Zhang L, Ren KJ. The study on the effect of Tangshenkang Granule on renal damage in diabetic nephropathy. J New Chin Med (Chin) 2006;38:44–45.

    Google Scholar 

  3. Wang L, Yu SJ. The experimental study on the method of preparing the model of type 2 diabetic nephropathy rats. Liaoning J Tradit Chin Med (Chin) 2011;38:1675–1677.

    Google Scholar 

  4. Yang YB, Zhang W, Su KL, Chen ZJ, Huang SM. Study on experimental diabetic nephropathy in rats induced by streptoeotocin. West China Med J (Chin) 2005;20:299–300.

    CAS  Google Scholar 

  5. Zhang JF, Meng M. Determination of polysaccharide in Tangshenkang Granules by itraviolet spectrophotometryl. J Anhui Univ Chin Med (Chin) 2005;24:48–49.

    Google Scholar 

  6. Chen Q, ed. Chinese medicine pharmacology research methodology. Beijing: People’s Medical Publishing House; 1993:1103.

    Google Scholar 

  7. Shi SS, Zhang YQ, Wen WB. Effects of liver ? receptor expression on rats with diabetic nephropathy and its mechanism. Chin Gen Pract (Chin) 2015;18:1029–1031.

    CAS  Google Scholar 

  8. Cheng H, Dong HR, Lin RQ, Sun LJ, Chen YP. Determination of normal value of glomerular size in Chinese adults by different measurement methods. Nephrology (Carlton) 2012;17:488–492.

    Article  Google Scholar 

  9. Zou WZ, ed. Pathology of renal biopsy. Beijing: Peking University Medical Press; 2014:160-162.

    Google Scholar 

  10. Giunti S, Barit D, Cooper ME. Mechanisms of diabetic nephropathy role of hypertension. Hypertension 2006;48:519–526.

    Article  PubMed  CAS  Google Scholar 

  11. Qi D, Xing Y, Zhang Y, Lu WR, Fan HY. The Protective effect of salvianolate combined with benazepril on diabetic nephropathy in streptozotocin-induced diabetic rats. Chin J Integr Tradit West Nephrol (Chin) 2012;13:314–316.

    Google Scholar 

  12. Xing SL, Zheng JF, Huang WZ. The study on diabetic nephropathy model in unilateral nephrectomy rats inducede by STZ. J Emerg Tradit Chin Med (Chin) 2006:15:643-644.

    Google Scholar 

  13. Song EF, Liu JJ, Jia RH, Wei GL, Ren KM. The study on the establishment of rat model of type 2 diabetic nephropathy. The J Pract Med (Chin) 2007;23:2840–2842.

    Google Scholar 

  14. Cao EZ, Yu XY, Hu SJ, Wang YP, Cheng W, Fang Q, et al. The effects of Tangshenkang Granule on early renal lesions in experimental diabetic rats. J Emerg Tradit Chin Med (Chin) 2002;3:448–450.

    Google Scholar 

  15. Yan LY, Yang SY, Li XJ. Research progress of the control mechanisms of Astragalus polysaccharides on diabetic nephropathy. Med Recapitulate (Chin) 2012;18:1727–1729.

    CAS  Google Scholar 

  16. Tan CQ. The therapeutic effect of Chinese yam polysaccharides on diabetic nephropathy in rats. Chin J Appl Physiol (Chin) 2014;30:437–438.

    CAS  Google Scholar 

  17. Huang L, Xie SF, Zhang L. Effects of Rhizoma dioscorea polysaccharide on renal function and MCP-1 expression in kidney of diabetic nephropathy rats. Chin Trop Med (Chin) 2013;13:1456–1459.

    CAS  Google Scholar 

  18. Wang JF. New progress of pharmacological research of prince ginseng. Pract Pharm Clin Remed (Chin) 2013;16:333–334.

    CAS  Google Scholar 

  19. Yang GQ, Zhang J, Ding XH, Zhang SQ. Astragalus, prince edward and rats to mesangial cells MMP-2 and TIMP-2— the influence of mRNA expression. Chin J Integr Tradit West Nephrol (Chin) 2011;12:673–675.

    Google Scholar 

  20. Gu LB, Wang YG, Wan M. Research progress on molecular mechanisms of Rhubarb in the treatment of diabetic nephropathy. Chin J Chin Mater Med (Chin) 2003;28:703–705.

    Google Scholar 

  21. Zhao SR, Lu YW, Chen JL, Duan HF, Wu ZZ. Experimental study on the hypoglycemic activity of catalpol from Rehmannia glutinosa Libosch. Lishizhen Med Mater Med Res (Chin) 2009;20:171–172.

    CAS  Google Scholar 

  22. Lu WB, Yang PJ, Li SM, Lu YP, Huang ZY, Huang H. Effect of salvianolate on inflammatory cytokines and renal vascular endothelial function in early diabetic nephropathy. Chin J Exp Tradit Med Form (Chin) 2014;20:184–187.

    CAS  Google Scholar 

  23. Liu DL, Ma ST. Research progress on pharmacological effects of total glucosides of paeony in the treatment of diabetic nephropathy. Chin J Integr Tradit West Med (Chin) 2013;33:1143–1146.

    Google Scholar 

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Authors and Affiliations

  1. Department of Internal Medicine, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, 230031, China

    Shun-jin Hu, Hua Jin & Ke-jun Ren

  2. Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China

    Bing Shu, Xiao-feng Li & Yong-jun Wang

  3. Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China

    Bing Shu, Xiao-feng Li & Yong-jun Wang

  4. Department of Pathology, Shanxi Hospital of Traditional Chinese Medicine, Xi’an, 710003, China

    Jia-rong Mao

  5. Graduate School, Anhui University of Traditional Chinese Medicine, Hefei, 230038, China

    Lei Gao, Li Yang & You-wan Wu

Authors
  1. Shun-jin Hu
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  2. Bing Shu
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  3. Hua Jin
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  4. Xiao-feng Li
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  5. Jia-rong Mao
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  7. Lei Gao
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  8. Li Yang
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  9. You-wan Wu
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  10. Yong-jun Wang
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Corresponding author

Correspondence to Shun-jin Hu.

Additional information

Supported by the Scientific Research Project of Colleges and Universities in Anhui Province, China (No. 2013z172) and the National Natural Science Foundation of China (No. 81503590)

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Hu, Sj., Shu, B., Jin, H. et al. Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy. Chin. J. Integr. Med. 24, 600–605 (2018). https://doi.org/10.1007/s11655-016-2607-x

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  • DOI: https://doi.org/10.1007/s11655-016-2607-x

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