Abstract
Objectives
To study the effect of Wenhua Juanbi Recipe (温化蠲痹方, WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and tumor necrosis factor receptor superfamily member 14 (TNFRSF14, also known as LIGHT) in rats with collagen-induced arthritis (CIA).
Methods
CIA rats were generated by subcutaneous injection of bovine collagen type-II at the tail base. Sixty CIA rats were randomly assigned (10 animals/group) to: model, methotrexate (MTX)-treated (0.78 mg/kg body weight), and WJR-treated (22.9 g/kg) groups. Healthy normal rats (n=10) were used as the normal control. Treatments or saline were administered once daily by oral gavage. Rats were sacrifificed at day 28 post-treatment and knee synovium and peripheral blood serum were collected. Toe swelling degree and expression of RANKL, OPG, and LIGHT were determined by Western blot and immunohistochemistry.
Results
Compared with the normal group, toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment, toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group, expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely, RANKL and LIGHT expression were signifificantly reduced and OPG increased in the WJR and MTX groups compared with the model group (P<0.01). No statistically significant difference existed between WJR and MTX groups.
Conclusion
WJR likely acts by reducing RANKL expression and increasing OPG expression, thus inhibiting RANKL/RANK interaction and reducing LIGHT expression, thereby inhibiting osteoclast formation/activation to block bone erosion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Firestein GS. Evolving concepts of rheumatoid arthritis. Nature 2003;423: 356–361.
Tanaka S. RANKL as a target molecule for treatment of joint destruction. Clin Calcium 2007;17: 586–592.
Boyce BF, Schwarz EM, Xing LP. Osteoclast precursors: cytokine-stimulated immunomodulators of inflammatory bone disease. Curr Opin Rheumatol 2006;18: 427–432.
Rauner M, Sipos W, Pietsehmann P. Osteoimmunology. Int Arch Allergy Immunol 2007;143: 31–48.
Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, et al. LIGHT (TNFSFl4), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Arthritis Rheum 2006;54: 1451–1462.
Anandrjah AP, Schwarz EM. Anti-RANKL therapy for inflammatory bone disorders: Mechanisms and potential clinical applications. J Cell Bicohem 2006;97: 226–232.
Liu XD, Zhang JL, Ye LH, Liu FY, Chen Y. Effects of Wenhua Juanbi Recipe on TNF-a and IL-1ß in peripheral blood of rheumatoid arthritis patients. Chin J Integr Tradit West Med (Chin) 2009;29: 789–793.
Schett G, Middleton S, Bolon B, Stolina M, Brown H, Zhu L, et al. Additive bone-protective effects of anabolic treatment when used in conjunction with RANKL and tumor necrosis factor inhibition in two rat arthritis models. Arthritis Rheum 2005;52: 1604–1611.
Carter RA, Campbell IK, O’Donnel KL, Wicks IP. Vascular cell adhesion molecule-1 (VCAM-1) blockade in collageninduced arthritis reduces joint involvement and alters B cell trafficking. Clin Exp Immunol 2002;128: 44–51.
Trentham DE, Townes AS, Kang AH. Autoimmunity to type ? collagen: an experimental model of arthritis. J Exp Med 1997;164: 857–867.
Okuzawa C, Kaneko Y, Murata Y, Miyake A, Saito Y, Okajo J, et al. Resistance to collagen-induced arthritis in SHPS-1 mutant mice. Biochem Biophys Res Commun 2008;371: 561–566.
Liu XD, Chen Y, Liu FY, Ye LH, Cai L. Effect of Wenhua Juanbi Recipe on proliferation and apoptosis of synoviocytes in rats with collagen-inducingarthritis. Chin J Integr Med 2013;19: 453–458.
Li W, Li L, Shi YY, Yin XJ, Xiao YQ. Experimental study of pharmacological action of active composition of Radix saposhnikovia. Chin J Exp Med Formul (Chin) 2006;2: 29–31.
Li XL, Zhang YQ, Huang TH. Study on anti-inflammatory and analgesic effects of extract from Brassica alb. Res Pract Chin Med (Chin) 2007;21: 28–30.
Li T, Li YM, Liu LF, Wang YX, Li FW, Zhu XX. Effective mechanism on anti-rheumatoid arthritis of total saponins clematis. J China Pharm Univ (Chin) 2009;40: 157–160.
Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature 2003;423: 337–342.
Sasaki N, Kusano E. Bone and bone related biochemical examinations. Bone and collagen related metabolites, Measurement and clinical role of OPG. Clin Calcium 2006;16: 956–962.
Sato K, Takayanagi H. Osteoclasts rheumatoid arthritis, and osteoimmunology. Curr Opin Rheumatol 2006;18: 419–426.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the Zhejiang Provincial Natural Science Foundation of China (No. LY12H29008); the Zhejiang Provincial Project of Traditional Chinese Medicine Science and Technology Plan (No. 2008CA086, 2009CB195, 2012ZB121, 2015ZA143); the Hangzhou Health Science and Technology Plan (No. 2010B027, No. 2014A37); the Hangzhou Social Development Plan (No. 20120633B12, No. 20160533B45)
Rights and permissions
About this article
Cite this article
Liu, Xd., Wang, Yq., Cai, L. et al. Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis. Chin. J. Integr. Med. 23, 208–214 (2017). https://doi.org/10.1007/s11655-016-2532-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11655-016-2532-z