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Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells

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Abstract

Objective

To observe the effects of Danhong Injection (丹红注射液) and its main components, including daiclzein and hydroxysafflor yellow A (HSYA), on the anticoagulation, fibrinolysis, anti-apoptosis in hypoxia model of vein endothelial cells (VECs).

Methods

VECs were prepared and were put in a hypoxia environment, which consisted of mixed gas of 95% N and 5% CO mixed gas, when reached confluent culture. Five groups used different treatments, including normal control group, hypoxia group, daiclzein group, HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection, daiclzein and HSYA on 6 keto prostaglandin F1α (6-keto-PGF1α) level was measured by the method of radioimmunoassay (RIA). Superoxide dismutase (SOD) activity was tested by water soluble tetrazolium salt. The content of malondialdehyde (MDA) was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) were measured by the method of chromogenic substrate. The contents of endothelin (ET) and nitric oxide (NO) were detected by non-equilibrium RIA and enzymelinked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry.

Results

Compared with the normal control group, the floating cells number, PAI activity, ET and MDA contents, and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased, whereas the 6-keto-PGF1α and NO contents, and t-PA and SOD activities were decreased significantly (P<0.01). Compared with the hypoxia group, Danhong Injection markedly increased the 6-keto-PGF1α content and SOD activity, regulated PAI and t-PA activities, ET and NO contents, and decreased MDA content and cells apoptosis rate (P<0.05 or P<0.01).

Conclusions

Danhong Injection and its main components played an important role in protecting primary VECs from hypoxic damage by regulating the secretion and vasomotor function of VECs. The function of Danhong Injection was most remarkable.

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Authors and Affiliations

  1. Research Institution of Cardio-Cerebral-Vascular Disease, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China

    Yu-yan Zhang  (张宇燕), Hui-fen Zhou  (周惠芬), Jie-hong Yang  (杨洁红) & Hai-tong Wan  (万海同)

  2. Pharmaceutical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, China

    Yu He  (何 昱) & Hao-fang Wan  (万浩芳)

  3. School of Food and Pharmaceutical Engineering, Hubei University of Technology, Wuhan, 430068, China

    Xiao-qiang Chen  (陈小强) & Nishinari Katsuyoshi

Authors
  1. Yu-yan Zhang  (张宇燕)
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  2. Hui-fen Zhou  (周惠芬)
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  3. Jie-hong Yang  (杨洁红)
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  4. Yu He  (何 昱)
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  5. Xiao-qiang Chen  (陈小强)
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  6. Nishinari Katsuyoshi
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  7. Hao-fang Wan  (万浩芳)
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  8. Hai-tong Wan  (万海同)
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Corresponding author

Correspondence to Hai-tong Wan  (万海同).

Additional information

Supported by the National Natural Science Foundation of China (No. 81173647, 81473587, 81274176), Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents, and Key Discipline of Zhejiang Traditional Chinese Medicine (Integrated Traditional and Western Medicine, No. 2012-XK-A06)

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Zhang, Yy., Zhou, Hf., Yang, Jh. et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells. Chin. J. Integr. Med. 22, 276–283 (2016). https://doi.org/10.1007/s11655-016-2498-x

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  • DOI: https://doi.org/10.1007/s11655-016-2498-x

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