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Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells

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Abstract

Objective

To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivationreperfusion (OGD/RO)-induced injury in SH-SY5Y cells.

Methods

SH-SY5Y cells were divided into a control group, a OGD/RO group, which was subject to OGD/RO induction; and 3 baicalin groups subject to baicalin (1, 5, 25 μmol/L) for 2 h before induction of OGD/RO (low-, medium-, and high-dose baicalin groups). Cell viability was detected by thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometric analysis was used to detect cell apoptosis. Real-time polymerase chain reaction was performed to determine the mRNA expression of caspase-3 gene. Western blot analysis was conducted to determine the expression of nuclear factor (NF)-κB and N-methyl-daspartic acid receptor-1 (NMDAR1).

Results

Baicalin could significantly attenuate OGD/RO mediated apoptotic cell death in SH-SY5Y cells; the apoptosis rates in the low-, medium- and high-dose groups were 12.1%, 7.9%, and 5.4%, respectively. Western blot and real-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD/RO group (P<0.01). Additionally, down-regulation of NF-κB and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD/RO group. Compared with the low-dose baicalin group, remarkable decrease was noted in the medium- and high-dose groups (P<0.01).

Conclusion

Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis.

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Authors and Affiliations

  1. Department of Neurology, the Second Hospital of Shandong University, Jinan, 250033, China

    Qing-bo Zhou  (周庆博), Xiao-ning Ju  (鞠小宁), Xiao-yun Wang  (王晓云), Mei-hong Wang  (王美红) & Jian-zhong Bi  (毕建忠)

  2. Central Laboratory, the Second Hospital of Shandong University, Jinan, 250033, China

    Feng Kong  (孔 峰) & Chao Sun  (孙 超)

Authors
  1. Qing-bo Zhou  (周庆博)
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  2. Xiao-ning Ju  (鞠小宁)
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  3. Xiao-yun Wang  (王晓云)
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  4. Mei-hong Wang  (王美红)
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  5. Feng Kong  (孔 峰)
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  6. Chao Sun  (孙 超)
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  7. Jian-zhong Bi  (毕建忠)
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Correspondence to Jian-zhong Bi  (毕建忠).

Additional information

Supported by Seed Fund of the 2nd Hospital of Shandong University (No. S2013010002).

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Zhou, Qb., Ju, Xn., Wang, Xy. et al. Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells. Chin. J. Integr. Med. 22, 201–206 (2016). https://doi.org/10.1007/s11655-015-2326-8

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  • DOI: https://doi.org/10.1007/s11655-015-2326-8

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