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In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study

Chinese Journal of Integrative Medicine volume 24, pages 606–612 (2018)Cite this article

Abstract

Objective

To compare the angiogenesis behaviors of vascular endothelial growth factor (VEGF) and Chinese medicine Xuefu Zhuyu Decoction (血府逐瘀汤, XZD) treatments.

Methods

Human microvascular endothelial cells (HMEC-1) were treated with various concentrations of either XZD-containing serum (XZD-CS) or VEGF for 24, 48, and 72 h, respectively. Cell viability, proliferation, migration, adhesion, and in vitro tube formation assays were used to assess their angiogenic effects.

Results

VEGF promoted all cellular phases involved in angiogenesis including cell viability, proliferation, migration, adhesion, and tube formation (<0.05 or <0.01). Unlike the continuous promotion effects of VEGF at the above stages, XZD inhibited cell viability and proliferation (<0.05 or <0.01) and only promoted tube formation in the early phase of angiogenesis (<0.01).

Conclusions

These two medications promote different angiogenesis behaviors, which might be an important reason for their distinct therapeutic profile in clinical usage.

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Author information

Affiliations

  1. College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China

    Fan Lin, Yi-zheng Wang & Dong Gao

  2. The Second Hospital of Longyan, Longyan, Fujian Province, 364000, China

    Bin-ling Chen

  3. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Jun Song

  4. Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA

    T. J. Kaptchuk

  5. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Ke-ji Chen

Authors
  1. Fan Lin
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  2. Bin-ling Chen
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  3. Yi-zheng Wang
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  4. Dong Gao
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  5. Jun Song
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  6. T. J. Kaptchuk
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  7. Ke-ji Chen
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Corresponding authors

Correspondence to Dong Gao or Jun Song.

Additional information

Supported by the National Natural Science Foundation of China (Nos. 81072933, 81173431 and 81102844); T. J. Kaptchuk was partially supported by National Center for Complementary and Alternative Medicine, National Institutes of Health, USA (No. 2K24 AT004095)

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Lin, F., Chen, Bl., Wang, Yz. et al. In Vitro Angiogenesis Effect of Xuefu Zhuyu Decoction (血府逐瘀汤) and Vascular Endothelial Growth Factor: A Comparison Study. Chin. J. Integr. Med. 24, 606–612 (2018). https://doi.org/10.1007/s11655-015-2289-9

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  • DOI: https://doi.org/10.1007/s11655-015-2289-9

Keywords

  • Xuefu Zhuyu Decoction
  • Chinese medicine
  • vascular endothelial growth factor
  • angiogenesis