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Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis

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Abstract

Objective

To study the influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis (EAU).

Methods

The EAU models were induced in Lewis rats by immunization with interphotoreceptor retinoid binding protein (IRBP) 1177–1191 in complete Freund's adjuvant. The rats were randomly divided into 3 groups: a model control group, a Yanyankang group, and a prednisone group, 9 rats in each group. The model control group was intervened with saline solution by gavage. The Yanyankang group was intervened with Yanyankang Powder 4 g/(kg day) by gavage. The prednisone group were intervened with prednisone acetate tablets 5 mg/(kg d) by gavage. All groups were intervened after immunization once every 2 days for 18 days and monitored by slit-lamp biomicroscopy daily until day 18. The levels of gamma interferon (INF-γ) and interleukin-10 (IL-10) in the supernatants of T cells were determined by enzyme-linked immunosorbent assay. Polymerase chain reaction (PCR) technology was used for measuring Th1 and Th2 related cytokine mRNA expressions.

Results

Slighter intraocular inflammation was found in the Yanyankang group and the prednisone group than the control group. The levels of the IFN-γand IL-10 in the supernatants of the spleen lymph node cells were 382.33±6.30, 155.87±4.46 μg/L in the Yanyankang group and 270.93±7.76, 265.32±11.88 μg/L in the prednisone group. Both had significant differences compared with the control group (941.53±8.59, 20.67±4.65 μg/L; =0.01). The PCR results showed the same tendency.

Conclusion

Yanyankang Powder showed favorable effects in the rats with EAU by influencing the function of Th1 and Th2 cells.

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Authors and Affiliations

  1. Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250002, China

    Qing-mei Tian  (田庆梅), Hong-sheng Bi  (毕宏生), Yan Cui  (崔 彦), Jian-feng Wu  (吴建峰) & Xiao-feng Xie  (解孝锋)

  2. Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, 250002, China

    Hong-sheng Bi  (毕宏生), Jun-guo Guo  (郭俊国) & Da-dong Guo  (郭大东)

  3. Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250000, China

    Qing-mei Tian  (田庆梅), Hong-sheng Bi  (毕宏生) & Ji-cun Qian  (钱继存)

Authors
  1. Qing-mei Tian  (田庆梅)
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  2. Hong-sheng Bi  (毕宏生)
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  3. Yan Cui  (崔 彦)
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  4. Jian-feng Wu  (吴建峰)
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  5. Xiao-feng Xie  (解孝锋)
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  6. Jun-guo Guo  (郭俊国)
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  7. Da-dong Guo  (郭大东)
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  8. Ji-cun Qian  (钱继存)
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Corresponding author

Correspondence to Hong-sheng Bi  (毕宏生).

Additional information

Supported by the National Natural Science Foundation of China (No. 81072961, 81100658 and 81373826) and the National Science Foundation for Post-doctoral Scientists of China (No. 2012M510068)

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Tian, Qm., Bi, Hs., Cui, Y. et al. Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis. Chin. J. Integr. Med. 22, 214–218 (2016). https://doi.org/10.1007/s11655-015-2182-6

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  • DOI: https://doi.org/10.1007/s11655-015-2182-6

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