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Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase

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Abstract

Objective

To compare the effect of Shen-Fu Injection (SFI) and epinephrine on the expression of sarcoplasmic reticulum Ca2+ ATPase 2a (SERCA2a) in a pig model with post-resuscitation myocardial dysfunction.

Methods

Ventricular fibrillation (VF) was electrically induced in Wu-zhi-shan miniature pigs. After 8 min of untreated VF and 2 min of cardiopulmonary resuscitation (CPR), all animals were randomly administered a bolus injection of saline placebo (SA group, n=10), SFI (0.8 mg/kg, SFI group, n=10) or epinephrine (20 μg/kg, EPI group, n=10). After 4 min of CPR, a 100-J shock was delivered. If the defibrillation attempt failed to attain restoration of spontaneous circulation (ROSC), manual chest compressions were rapidly resumed for a further 2 min followed by a second defibrillation attempt. Hemodynamic variables were recorded, and plasma concentrations of catecholamines were measured. Adenylate cyclase (AC), cyclic adenosine monophosphate (cAMP) and the expressions of β1-adrenoceptor (AR) and SERCA 2a were determined.

Results

Cardiac output, left ventricular dp/dtmax and negative dp/dtmax were significantly higher in the SFI group than in the SA and EPI groups at 4 and 6 h after ROSC. The expression of β1-AR and SERCA2a at 24 h after ROSC were significantly higher in the SFI group than in the SA and EPI groups (P<0.05 or P<0.01).

Conclusions

The administration of epinephrine during CPR decreased the expression of SERCA2a and aggravated postresuscitation myocardial function (P<0.01). SFI attenuated post-resuscitation myocardial dysfunction, and the mechanism might be related to the up-regulation of SERCA2a expression.

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Authors and Affiliations

  1. Intensive Care Unit, Shandong Cancer Hospital and Institute, Jinan, 250117, China

    Zhi-jun Guo  (郭志军)

  2. Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China

    Zhi-jun Guo  (郭志军), Cai-jun Wu  (吴彩军) & Chun-sheng Li  (李春盛)

Authors
  1. Zhi-jun Guo  (郭志军)
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  2. Cai-jun Wu  (吴彩军)
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  3. Chun-sheng Li  (李春盛)
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Correspondence to Chun-sheng Li  (李春盛).

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Guo, Zj., Wu, Cj. & Li, Cs. Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase. Chin. J. Integr. Med. 22, 503–509 (2016). https://doi.org/10.1007/s11655-015-2156-8

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  • DOI: https://doi.org/10.1007/s11655-015-2156-8

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