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Chinese Journal of Integrative Medicine

, Volume 21, Issue 4, pp 291–298 | Cite as

Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats

  • Tian-yang Liu (刘天阳)
  • Ling-ling Zhou (周玲玲)Email author
  • Cong Zhou (周 聪)
  • Zhang-pu Liu (柳璋璞)
  • Chen Chen (陈 晨)
  • Zhe Feng (冯 哲)
  • Xue-ping Zhou (周学平)Email author
Original Article

Abstract

Objective

To study the mechanism underlying the inhibitory effect of Qingluo Tongbi Granule (清络通痹颗粒, QTG) on osteoclast differentiation in rheumatoid arthritis in rats.

Methods

Fibroblast and monocyte co-culture were used to induce osteoclast differentiation in adjuvant-induced arthritic (AIA) rats. Serum containing QTG was prepared and added to the osteoclasts, and activation of the tumor necrosis factor receptor-associated factor 6/mitogen-activated protein kinase/nuclear factor of activated T cells, cytoplasmic1 (TRAF6/MAPK/NFATc1) pathways was examined.

Results

The induced osteoclasts were multinucleated and stained positive for tartrate-resistant acid phosphatase (TRAP) staining. Serum containing QTG at 14.4, 7.2 or 3.6 g/kg inhibited the activation of TRAF6, extracellular regulated protein kinase (ERK)1/2, c-Jun N-terminal kinase (JNK) and p38 and decreased the percentage of cells with nuclear NFATc1 in a dose-dependent manner, the high and middle doses exhibited clear inhibitory activity (P<0.01 and P<0.05, respectively). After the addition of MAPK inhibitors, the NFATc1 expression showed no significant difference compared with the control group (P>0.05).

Conclusions

Serum containing QTG could generally inhibit the TRAF6/MAPK pathways and possibly inhibit the NFATc1 pathway. In addition, QTG may regulate other signaling pathways that are related to osteoclast differentiation and maturation.

Keywords

Qingluo Tongbi Granule rheumatoid arthritis osteoclast differentiation signaling pathway regulation of tumor necrosis factor receptor-associated factor 6/mitogen-activated protein kinase pathways Chinese medicine 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Tian-yang Liu (刘天阳)
    • 1
    • 2
  • Ling-ling Zhou (周玲玲)
    • 1
    Email author
  • Cong Zhou (周 聪)
    • 1
  • Zhang-pu Liu (柳璋璞)
    • 1
  • Chen Chen (陈 晨)
    • 1
  • Zhe Feng (冯 哲)
    • 1
  • Xue-ping Zhou (周学平)
    • 1
    Email author
  1. 1.Nanjing University of Chinese MedicineNanjingChina
  2. 2.Department of Rheumatology and ImmunologyThe First Affiliated Hospital of Anhui University of Chinese MedicineHefeiChina

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