Effects of royal jelly supplementation on glycemic control and oxidative stress factors in type 2 diabetic female: A randomized clinical trial

Abstract

Objective

It has been proposed that royal jelly has antioxidant properties and may improve oxidative stress and glycemic control. Therefore, we investigated the effects of royal jelly supplementation in diabetic females.

Methods

In this pilot, parallel design randomized clinical trial, 50 female volunteers with type 2 diabetes were randomly allocated to the supplemented (25, cases) and placebo (25, cases) groups, based on random block procedure produced by Random Allocation Software, given a daily dose of 1,000 mg royal jelly soft gel or placebo, respectively, for 8 weeks. Before and after intervention, glycemic control indices, antioxidant and oxidative stress factors were measured.

Results

After royal jelly supplementation, the mean fasting blood glucose decreased remarkably (163.05±42.51 mg/dL vs. 149.68±42.7 mg/dL). Royal jelly supplementation resulted in significant reduction in the mean serum glycosylated hemoglobin levels (8.67%±2.24% vs. 7.05%±1.45%, P=0.001) and significant elevation in the mean insulin concentration (70.28±29.16 pmol/L vs. 86.46±27.50 pmol/L, P=0.01). Supplementation significantly increased erythrocyte superoxidase dismutase and glutathione peroxidase activities and decreased malondialdehyde levels (P<0.05). At the end of study, the mean total antioxidant capacity elevated insignificantly in both groups.

Conclusions

On the basis of our findings, it seems that royal jelly supplementation may be beneficial in controlling diabetes outcomes. Further studies with larger sample size are warranted.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Khuwaja AK, Khowaja LA, Cosgrove P. The economic costs of diabetes in developing countries: some concerns and recommendations. Diabetologia 2010;53:389–390.

    CAS  PubMed  Article  Google Scholar 

  2. 2.

    Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047–1053.

    PubMed  Article  Google Scholar 

  3. 3.

    Glugliano D, Paolisso G, Ceriello A. Oxidative stress and diabetes vascular complications. Diabetes Care 1996;19:257–267.

    Article  Google Scholar 

  4. 4.

    Roussel A, Kerkeni, Zouari N, Mahjoub S, Matheau J, Richard A. Antioxidant effects of zinc supplementation in Tunisians with type 2 diabetes mellitus. J Am Coll Nutr 2003;22:4:316–321.

    CAS  PubMed  Article  Google Scholar 

  5. 5.

    Bonnefont-roussel D, Batrad JP, Jaudon MC, Delattre J. Consequences of the diabetic status on the oxidant/antioxidant balance. Diabetes Metab 2000;26:163–176.

    Google Scholar 

  6. 6.

    Johansen JS, Harris AK, Rychly DJ, Ergul A. Oxidative stress and the use of antioxidants in diabetes: linking basic science to clinical practice. Cardiovasc Diabetol 2005;4(1):5.

    PubMed Central  PubMed  Article  Google Scholar 

  7. 7.

    Neri S, Signorelli SS, Torrisi B, Pulvirenti D, Mauceri B, Abate G, et al. Effects of antioxidant supplementation on postprandial oxidative stress and endothelial dysfunction: a single-blind, 15-day clinical trial in patients with untreated type 2 diabetes, subjects with impaired glucose tolerance, and healthy controls. Clin Ther 2005;27:1764–1773.

    CAS  PubMed  Article  Google Scholar 

  8. 8.

    Vega-Lopez S, Devaraj S, Jialal I. Oxidative stress and antioxidant supplementation in the management of diabetic cardiovascular disease. J Investig Med 2004;52:24–32.

    CAS  PubMed  Article  Google Scholar 

  9. 9.

    Lee DH, Falsom AR, Harnack L, Halliwell B, Jacobs Jr DR. Does supplemental vitamin C increase cardiovascular disease risk in women with diabetes? Am J Clin Nutr 2004;80:1194–1200.

    CAS  PubMed  Google Scholar 

  10. 10.

    Fujii A, Kobayashi S, Kuboyama N, Furukawa Y, Kaneko Y, Ishihama S, et al. Augmentation of wound healing by royal jelly (RJ) in streptozotocin-diabetic rats. Jpn J Pharmacol 1990;53:331–337.

    CAS  PubMed  Article  Google Scholar 

  11. 11.

    Suzuki KM, Isohama Y, Maruyama H, Yamada Y, Narita Y, Ohta SH, et al. Estrogenic activities of fatty acids and a sterol isolated from Royal Jelly. Evid Based Complement Alternat Med 2008;5:295–302.

    PubMed Central  PubMed  Article  Google Scholar 

  12. 12.

    El-Nekeety AA, El-Kholy W, Abbas NF, Ebaid A, Amra HA, Abdel-Wahhab MA. Efficacy of royal jelly against the oxidative stress of fumonisin in rats. Toxicon 2007;50:256–269.

    CAS  PubMed  Article  Google Scholar 

  13. 13.

    Inoue SI, Koya-Miyata S, Ushio S, Iwak K, Ikeda M, Kurimoto M. Royal jelly prolongs the life span of C3H/H3J mice: correlation with reduced DNA damage. Exp Gerontol 2003;38:965–969.

    CAS  PubMed  Article  Google Scholar 

  14. 14.

    Tokunaga KH, Yoshida CH, Suzuki KM, Maruyama H, Futamura Y, Araki Y, et al. Antihypertensive effect of peptides from Royal Jelly in spontaneously hypertensive rats. Biol PharmBull 2004;27:189–192.

    CAS  Article  Google Scholar 

  15. 15.

    Gou H, Sagia A, Sato M, Miyazawa I, Shibata M, Takahata Y, et al. Royal jelly supplementation improve lipoprotein metabolism in humans. J Nutr Sci Vitaminol 2007;53:354–348.

    Article  Google Scholar 

  16. 16.

    Kohno K, Okamoto I, Sano O, Arai N, Iwaki K, Ikeda M, et al. Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages. Biosci Biotechnol Biochem 2004;68:138–145.

    CAS  PubMed  Article  Google Scholar 

  17. 17.

    Towsend GF, Morgan JF, Hazlett B. Activity of 10-hydroxydecenoic acid from royal jelly against experimental leuacemia and ascitic tumors. Nature 1959;183:1270–1271.

    Article  Google Scholar 

  18. 18.

    Nagai T, Inoue R. Preparation and the functional properties of water extract and alkaline extract from royal jelly. Food Chem 2004;84:181–186.

    CAS  Article  Google Scholar 

  19. 19.

    Munstedt K, Baregello M, Hauenschild A. Royal jelly reduce the serum glucose levels in healthy subjects. J Med Food 2009;12(5):1–3.

    Article  Google Scholar 

  20. 20.

    Dixit PK, Patel NG. Insulin-like activity in larval foods of the honeybee. Nature Lond 1964;202:189–190.

    CAS  PubMed  Article  Google Scholar 

  21. 21.

    Kramer KJ, Tager HS. Insulin-like and glucagonlike peptides in insect hemolymph Insect. Biochem 1980;10:179–182.

    CAS  Google Scholar 

  22. 22.

    Woerle HJ, Neumann Ch, Zschau S, Tenner S, Irsigler A, Schirra J, et al. Impact of fasting and postprandial glycemia on overall glycemic control in type 2 diabetes importance of postprandial glycemia to achieve target HbA1c levels. Diabetes Res Clin Pract 2007;77:280–285.

    CAS  PubMed  Article  Google Scholar 

  23. 23.

    Gou H, Ekusa A, Iwai K, Yonekura M, Takahata Y, Morimatsu F. Royal jelly peptides inhibit lipid peroxidation in in vitro and in vivo. J Nutr Sci Vitaminol 2008;54:191–195.

    Article  Google Scholar 

  24. 24.

    Jamnik P, Goranovic D, Raspor P. Antioxidative action of royal jelly in the yeast cell. Exp Gerontol 2007;42:594–600.

    CAS  PubMed  Article  Google Scholar 

  25. 25.

    Kanbur M, Eraslan G, Beyaz L. The effects of royal jelly on liver damage induced by paracetamol in mice. Exp Toxicol Pathol 2009;61:123–132.

    CAS  PubMed  Article  Google Scholar 

  26. 26.

    Silici S, Ekmekcioglu O, Eraslan G, Demirtas A. Antioxidative effect of Royal Jelly in cisplatin-induced testes damage. Urology 2009;74:545–551.

    PubMed  Article  Google Scholar 

  27. 27.

    Gou H, Yoshiaki A, Yonekura M. Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chem 2009;113:238–245.

    Article  Google Scholar 

  28. 28.

    Kanbur M, Eraslan G, Silici S, Karababack M. Effects of sodium fluoride exposure on some biochemical parameters in mice: Evaluation of the ameliorative effect of royal jelly applications on these parameters. Food Chem Toxicol 2009;47:1184–1189.

    CAS  PubMed  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Reza Mahdavi.

Additional information

Support by the Nutrition Research Center, Tabriz University of Medical Sciences

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Pourmoradian, S., Mahdavi, R., Mobasseri, M. et al. Effects of royal jelly supplementation on glycemic control and oxidative stress factors in type 2 diabetic female: A randomized clinical trial. Chin. J. Integr. Med. 20, 347–352 (2014). https://doi.org/10.1007/s11655-014-1804-8

Download citation

Keywords

  • glycemic control
  • oxidative stress
  • Royal jelly
  • type 2 diabetes