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Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines

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Abstract

Objectives

Chunghyuldan (CHD), a combinatorial drug that has anti-hyperlipidemic and antiinflammatory activities, has been shown to reduce infarct volume in a focal ischemia-reperfusion rat model. To explore the molecular basis of CHD’s neuroprotective effect, we examined whether CHD shows a cell-protective activity and has a regulatory effect on Bax and/or B-cell leukemia/lymphoma 2 (Bcl-2) expression in mouse neuroblastoma 2a (N2a) cells subjected to hypoxia-reoxygenation (H/R).

Methods

In order to evaluate the effects of CHD on the cytotoxicity induced from hypoxia or H/R condition, lactate dehydrogenase (LDH) assay was performed. To explore whether the suppression of neural damage when pre-treated with CHD is associated with its anti-apoptotic effect, the CHD effect on the expression of Bcl-2 and Bax was analyzed by Western blotting analysis.

Results

Cytotoxicity of N2a cell line was slightly increased in 42 h hypoxia condition and dramatically increased under the H/R condition. CHD treatment markedly decreased the cytotoxicity in both conditions (P<0.01, P<0.05). H/R markedly increased the expression of the pro-apoptotic protein, Bax, but slightly increased the expression of the anti-apoptotic protein, Bcl-2, compared with the normoxia or hypoxia group. CHD significantly decreased Bax expression (P<0.01) and slightly decreased Bcl-2 expression (P>0.05), resulted in a reduction of Bax/Bcl-2 ratio in N2a cells subjected to H/R.

Conclusion

CHD has neuroprotective effect in N2a cells subjected to H/R, which might be derived at least in part from its ability to decrease the expression of the pro-apoptotic protein, Bax.

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Authors and Affiliations

  1. Department of Cardiology and Neurology Diseases, College of Korean Medicine, Kyung Hee University, Seoul, Korea

    Chang-Nam Ko, In-Seo Park, Seong-Uk Park, Woo-Sang Jung, Sang-Kwan Moon, Jung-Mi Park & Ki-Ho Cho

  2. Department of Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Korea

    Chulhun Kang

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  1. Chang-Nam Ko
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  2. In-Seo Park
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  3. Seong-Uk Park
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  4. Woo-Sang Jung
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  5. Sang-Kwan Moon
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  6. Jung-Mi Park
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  7. Chulhun Kang
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  8. Ki-Ho Cho
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Correspondence to Jung-Mi Park.

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Ko, CN., Park, IS., Park, SU. et al. Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines. Chin. J. Integr. Med. 19, 940–944 (2013). https://doi.org/10.1007/s11655-013-1657-6

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  • DOI: https://doi.org/10.1007/s11655-013-1657-6

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