Abstract
Objective
To observe the effect of Yiqixue Buganshen recipe(益气血补肝肾方, YBR) on the expression of integrin ανβ3 in the endometrium of controlled ovarian hyperstimulation mice.
Methods
A total of 180 mice were divided into three groups: model group, treatment group and control group. The treatment and model groups were intraperitoneally injected with gonadotropin-releasing hormone analogue for 7 days; pregnant mare serum gonadotropin was also injected on the 7th day. After 48 h, human chorionic gonadotropin was injected. The control group was injected with an equal volume of saline at the same time. From the start of the experiment, the treatment group was intragastrically administered Jinghouzengzhi Recipe(经后增殖方) and Cuhuangti Recipe(促黄体方). The model group and the control group were intragastrically administered an equal volume of saline. Real-time reverse transcription polymerase chain reaction and Western blotting were used to detect the mRNA and protein expression of integrin ανβ3 in mouse endometrium.
Results
Integrin ανβ3 was expressed in mouse endometrium in all groups. Integrin αββ3 expression increased gradually along with pregnancy, progressing from pregnant day (Pd) 1. Integrin ανβ3 expression significantly increased on Pd 4, then began to decrease on Pd 6. Integrin ανβ3 expression in the treatment group was higher than in the model group, and the difference was statistically significant (P <0.05). The difference between the treatment group and the control group was not statistically significant (P >0.05).
Conclusion
YBR improves endometrial receptivity, and may play an important role in embryonic implantation.
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References
Ma WG, Song H, Das SK, Paria BC, Dey SK. Estrogen is a critical determinant that specifies that duration of the window of uterine receptivity for implantation. Proc Natl Acad Sci USA 2003;100:2963–2968.
Horcajadas JA, Riesewijk A, Polman J, van Os R, Pellicer A, Mosselman S, et al. Effect of controlled ovarian hyperstimulation in IVF on endometrial gene expression profiles. Molecular Hum Reprod 2005;11:195–205.
Oodi J, Creus M, Quinto L, Casamitjana R, Cardesa A, Balasch J. Within-subject between-cycle variability of histological dating, alphaVbeta3 integrin expression, and pinopod formation in the human endometrium. J Clin Endocrinol Metab 2003;88:2119–2125.
Thomas K, Thomson A, Wood S, Kingsland C, Vince G, Lewis-Jones I. Endometrial integrin expression in women undergoing in vitro fertilization and association with subsequent treatment outcome. Fertil Steril 2003;80:502–507.
Srinivasan KR, Blesson CS, Fatima I, Kitchlu S, Jain SK, Mehrotra PK, et al. Expression of αvβ3 integrin in rat endometrial epithelial cells and its functional role during implantation. Gen Comp Endocrinol 2009;160:124–133.
Lessey BA. Endometrial integrins and the establishment of uterine receptivity. Hum Reprod 1998;13:247–258;discussion 259–261.
Achache H, Revel A. Endometrial receptivity markers, the journey to successful embryo implantation. Hum Reprod Update 2006;12:731–746.
Check J H, Nowroozi K, Chase J, Nazari A, Braithwaite C. Comparison of pregnancy rates following in vitro fertilization embryo transfer between the donors and the recipients in a donor oocyte program. J Assist Reprod Genet 1992;9:248–250.
Ertzeid G, Storeng R. The impact of ovarian stimulation on implantation and fetal development in mice. Hum Reprod 2001;16:221–225.
Garcia JE, Acosta AA, Hsiu JG, Jones HW Jr. Advanced endometrial maturation after ovulation induction with human menopausal gonadotropin/human chorionic gonadotropin for in vitro fertilization. Fertil Steril 1984;41:31–35.
Kolb BA, Najmabadi S, Paulson RJ. Ultrastructural characteristics of the luteal phase endometrium in donors undergoing controlled ovarian hyperstimulation. Fertil Steril 1997;67:625–630.
Simón C, Garcia Velasco JJ, Valbuena D, Peinado JA, Moreno C, Remohí J, et al. Increasing uterine receptivity by decreasing estradiol levels during the preimplantation period in high responders with the use of a follicle-stimulating hormone step-down regimen. Fertil Steril 1998;70:234–239.
Valbuena D, Jasper M, Remohi J, Pellicer A and Simón C. Ovarian stimulation and endometrial receptivity. Hum Reprod 1999;14:107–111.
Thomas K, Thomson AJ, Sephton V, Cowan C, Wood S, Vince G, et al. The effect of gonadotrophic stimulation on integrin expression in the endometrium. Hum Reprod 2002;17:63–68.
Horcajadas JA, Diaz-Gimeno P, Pellicer A, Simón C. Uterine receptivity and the ramifications of ovarian stimulation on endometrial function. Semin Reprod Med 2007;25:454–460.
Chen QJ, Sun XX, Li L, Gao XH, Gemzell-Danielsson K, Cheng LN. Effects of ovarian stimulation on endometrial integrin beta3 and leukemia inhibitory factor expression in the peri-implantation phase. Fertil Steril 2008;89:1357–1363.
Klein KO, Janfaza M, Wong JA, Chang RJ. Estrogen bioactivity in fo-ti and other herbs used for their estrogen-like effects as determined by a recombinant cell bioassay. J Clin Endocrinol Metab 2003;88:4077–4079.
Tamir S, Eizenberg M, Somjen D, Stern N, Shelach R, Kaye A, et al. Estrogenic and antiproliferative properties of glabridin from licorice in human breast cancer cells. Cancer Res 2000;60:5704–5709.
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Li, Hx., Guo, Xy., Xie, Y. et al. Yiqixue Buganshen Recipe (益气血补肝肾方) regulates the expression of integrin ανβ3 in the endometrium of controlled ovarian hyperstimulation mice. Chin. J. Integr. Med. 19, 755–760 (2013). https://doi.org/10.1007/s11655-013-1463-1
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DOI: https://doi.org/10.1007/s11655-013-1463-1