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Neutroprotective efficacy of sodium tanshinone B on hippocampus neuron in a rat model of focal cerebral ischemia

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Abstract

Objective

To investigate the protective effects of sodium tanshinone B (STB) on brain damage following focal ischemia-reperfusion (I/R) injury through interfering with N-methyl-D-aspartic acid receptor (NMDAR) and excitatory and inhibitory amino acids, and evaluate the potential mechanisms of the neuroprotective activity of STB.

Methods

Transient forebrain ischemia was induced by middle cerebral artery occlusion (MCAO). The rats were randomized into a sham operated group, a model group (I/R) and three STB different dose groups. Rats were pretreated with STB at the doses of 4, 8, 16 mg/kg (STB1, STB2, STB3) for 3 days before MCAO. The expression of NMDAR1 was detected by immunohistochemistry and Western blotting. The concentrations of glutamate and γ-aminobutyric acid (GABA) were analyzed using high performance liquid chromatography.

Results

STB treatment reduced neurological defect scores, cerebral infarction volume and brain water content. The levels of NMDAR1 were significantly higher in the l/R and STB1 groups than that of the sham and the STB3 groups (P<0.01). Optical density of NMDAR1 was significantly increased in cornu ammonis (CA)1 region of the l/R group (P<0.05). STB treatment reduced NMDAR1 optical density in the CA1 region (P<0.01). The levels of glutamate were significantly lower in the hippocampus in the STB3 group than that of the l/R, STB1 and STB2 groups (P<0.01).

Conclusion

Preconditioning with STB appears to be a simple and promising strategy to reduce or even prevent cerebral l/R injury and has potential for future clinical application.

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Authors and Affiliations

  1. Department of Physiology, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China

    Qing Cai  (蔡 青), Jun-zhen Tan  (谭俊珍), Tao Zhou  (周 涛) & Chun-shen Li  (李春深)

  2. College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China

    Hong-wu Wang  (王洪武)

  3. Department of Experiment, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China

    Sheng-yu Hua  (华声瑜)

Authors
  1. Qing Cai  (蔡 青)
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  2. Hong-wu Wang  (王洪武)
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  3. Sheng-yu Hua  (华声瑜)
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  4. Jun-zhen Tan  (谭俊珍)
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  5. Tao Zhou  (周 涛)
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  6. Chun-shen Li  (李春深)
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Corresponding author

Correspondence to Qing Cai  (蔡 青).

Additional information

Supported by Science Development Foundation of Tianjin Institute of Education (No. 20070301) and Tianjin Natural Science Foundation of China (No. 11JCYBJC13400)

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Cai, Q., Wang, Hw., Hua, Sy. et al. Neutroprotective efficacy of sodium tanshinone B on hippocampus neuron in a rat model of focal cerebral ischemia. Chin. J. Integr. Med. 18, 837–845 (2012). https://doi.org/10.1007/s11655-012-1266-9

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  • DOI: https://doi.org/10.1007/s11655-012-1266-9

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