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Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells

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Abstract

Objective

To investigate the protective effects of the natural medicinal monomer isopsoralen (ISR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide (H2O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects.

Methods

HLE-B3 cells were treated with H2O2 (300 μ mol/L), β-estradiol (E2: 10−8 mol/L) and H2O2, ISR (10−5 mol/L) and H2O2, or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surfaceenhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (m/z) ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test.

Results

H2O2 up-regulated the expressions of two protein spots (with m/z of 6532 and 6809). E2 mitigated the oxidative damage, and the expression of one protein spot (m/z 6532) was down-regulated. In contrast, ISR down-regulated both of protein spots (m/z 6532 and 6809).

Conclusions

ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2.

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References

  1. Klein BE, Klein R, Linton KL. Prevalence of age related lens opacities in a population. The Beaver Dam Eye Study. Ophthalmol 1992;99:546–552.

    CAS  Google Scholar 

  2. Cuming RG, Mitchell P. Hormone replacement therapy, reproductive factors, and cataract. The Bluc Mountains Eye Study. Am J Epidemiol 1997;145:242–249.

    Article  Google Scholar 

  3. Dolatowska E. The evaluation of estradiol and FSH serum levels in menopausal women with primary cataract. Klin Oczna 2002;104:357–361.

    PubMed  CAS  Google Scholar 

  4. Wang X, Simpkins JW, Dykens JA, Cammarata PR. Oxidative damage to human lens epithelial cells in culture: estrogen protection of mitochondrial potential, ATP, and cell viability. Invest Ophthalmol Vis Sci 2003;44:2067–2075.

    Article  PubMed  Google Scholar 

  5. Moor AN, Flynn JM, Gottipati S, Giblin FJ, Cammarata PR. 17beta-estradiol stimulates MAPK signaling pathway in human lens epithelial cell cultures preventing collapse of mitochondrial membrane potential during acute oxidative stress. Mitochondrion 2005;5:235–247.

    Article  PubMed  CAS  Google Scholar 

  6. Moor AN, Gottipati S, Mallet RT, Sun J, Giblin FJ, Roque R, Cammarata PR. A putative mitochondrial mechanism for antioxidative cytoprotection by 17 betaestradiol. Exp Eye Res 2004;78:933–944.

    Article  PubMed  CAS  Google Scholar 

  7. Flynn JM, Cammarata PR. Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells. Mol Vis 2006;12:271–282.

    PubMed  CAS  Google Scholar 

  8. Gottipati S, Cammarata PR. Mitochondrial super-oxide dismutase activation with 17 β-estradiol-treated human lens epithelial cells. Mol Vis 2008;14:898–905.

    PubMed  CAS  Google Scholar 

  9. Zhao PW, Wang DW, Wang LQ. Expression study in screening estrogen-like ability of ten traditional Chinese drugs using uterus increased weight. J Beijing Univ Tradit Chin Med (Chin) 2006;29:686–689.

    Google Scholar 

  10. Zhao PW, Niu JZ, Wang JF. Influence of Isopsoralen and ecdysterone in proliferation of human breast carcinoma cell. J Beijing Univ Tradit Chin Med (Chin) 2007;30:242–245.

    CAS  Google Scholar 

  11. Qi MX, Huang XR, Zhang KL, Guo N. Isopsoralen protecting against apoptosis of human lens epithelial cell. Chin J Clin Pharmacol Ther (Chin) 2009;14:1371–1374.

    Google Scholar 

  12. Huang X, Qi M, Zhang K, Guo N. Upregulatory effect of isopsoralen on expression of estrogen receptor in human lens epithelial cell. Chin Pathophysioloy (Chin) 2010;26:1844–1848.

    CAS  Google Scholar 

  13. Feng CY, Huang XR, Qi MX, Tang SW, Guo N, Hu YH. The protective effects and mechanisms of Isopsoralen in oxidative damage of human lens epithelial cell. China J Ophthalmol (Chin) 2011;47:353–355.

    Google Scholar 

  14. Hu YH, Huang XR, Qi MX, Hou BY. Study on proteomics of inhibitory effects of elemene on proliferation of human lens epithelial cell. China J Ophthalmol (Chin) 2010;46:427–431.

    CAS  Google Scholar 

  15. Nordgaard CL, Karunadharma PP, Feng X, Olsen TW, Ferrington DA. Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age-related macular degeneration. Invest Ophthalmol Vis Sci 2008;49:2848–2855.

    Article  PubMed  Google Scholar 

  16. Saraswathy S, Rao NA. Mitochondrial proteomics in experimental autoimmune uveitis oxidative stress. Invest Ophthalmol Vis Sci 2009;50:5559–5566.

    Article  PubMed  Google Scholar 

  17. Zhang Q, Liu Y, Ren YF. Detection of distinct protein in renal cell carcinoma cell line and normal kidney cell line by SELDI-TOFMS technology. Applied J Gen Pract (Chin) 2008;6:333–334.

    Google Scholar 

  18. Ge Z, Zhu YL, Zhong X. The influence of Helicobacter pylori associated protein A in human gastric adenocarcinoma epithelium cell proteome. Cell Biology 2006;28:603–610.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China

    Chun-yan Feng  (冯春燕)

  2. Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China

    Xiu-rong Huang  (黄秀榕) & Song-wen Tang  (唐宋文)

  3. Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China

    Chun-yan Feng  (冯春燕), Ming-xin Qi  (祁明信), Yan-hong Hu  (胡艳红), Sheng Chen  (陈 胜) & Fa-jie Ke  (柯发杰)

Authors
  1. Chun-yan Feng  (冯春燕)
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  2. Xiu-rong Huang  (黄秀榕)
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  3. Ming-xin Qi  (祁明信)
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  4. Song-wen Tang  (唐宋文)
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  5. Yan-hong Hu  (胡艳红)
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  6. Sheng Chen  (陈 胜)
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  7. Fa-jie Ke  (柯发杰)
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Corresponding author

Correspondence to Xiu-rong Huang  (黄秀榕).

Additional information

Supported by the Foundation of Fujian Province Health Department Fund (No.2009-1-30) and Fujian Provincie Department of Education Issues (No. JA10176)

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Feng, Cy., Huang, Xr., Qi, Mx. et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells. Chin. J. Integr. Med. 18, 529–533 (2012). https://doi.org/10.1007/s11655-012-1144-5

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  • DOI: https://doi.org/10.1007/s11655-012-1144-5

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