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Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice

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Abstract

Objective

To observe the effect of Huxin Formula (护心方, HXF) on expressions of the chief reverse cholesterol transport (RCT) associated genes, caveolin-1 and scavenger receptor-BI (SR-BI) in ApoE-gene knockout [ApoE (−/−)] mice.

Methods

Thirty ApoE (−/−) mice of 4–6 weeks old were randomly divided into three groups (A-C). After being fed with high-fat diet for 16 weeks, they were treated with HXF (1 mL/100 g), pravachol (0.3 mg/100 g), and saline in equal volume respectively for 16 weeks successively; in addition, a blank group was set up with 10 C57BL/6J mice of 6-week old received 16-week high-fat feeding and saline treatment. Animals were sacrificed at the termination of the experiment, their paraffin sections of aortic tissue were used to measure the size of plaque, expressions of cavolin-1 and SR-BI were detected by immunological histochemical method.

Results

As compared with the blank group, levels of caveolin-1 and SR-BI were increased in Groups A and B (P<0.01); but the increase in Group A was more significant than that in Group B (P<0.05). The plaque/aorta area ratio decreased significantly in Groups A and B, but showed insignificant difference between the two groups.

Conclusion

HXF could obviously increase the expressions of RCT associated genes, caveolin-1 and SR-BI, promote the RCT process, so as to reduce the formation of aorta atherosclerotic plaque in ApoE (−/−) mice.

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Authors and Affiliations

  1. The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510120, China

    Wei Jiang  (江 巍), Song Li  (李 松), Wei Mao  (毛 炜), Guang Yang  (杨 广), Xin-mei Li  (李新梅), Guang-juan Zheng  (郑广娟), Huan-lin Wu  (吴焕林) & Xin-min Ruan  (阮新民)

  2. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Ke-ji Chen  (陈可冀)

Authors
  1. Wei Jiang  (江 巍)
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  2. Song Li  (李 松)
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  3. Wei Mao  (毛 炜)
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  4. Guang Yang  (杨 广)
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  5. Xin-mei Li  (李新梅)
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  6. Guang-juan Zheng  (郑广娟)
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  7. Huan-lin Wu  (吴焕林)
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  8. Xin-min Ruan  (阮新民)
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  9. Ke-ji Chen  (陈可冀)
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Corresponding author

Correspondence to Wei Jiang  (江 巍).

Additional information

Supported by the National Natural Science Foundation of China (No. 81102584) and Guangdong Provincial Administration of Traditional Chinese Medicine (No. 2009386)

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Jiang, W., Li, S., Mao, W. et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice. Chin. J. Integr. Med. 18, 451–456 (2012). https://doi.org/10.1007/s11655-012-1123-x

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  • DOI: https://doi.org/10.1007/s11655-012-1123-x

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