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The preventive effect of garlicin on a porcine model of myocardial infarction reperfusion no-reflow

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Abstract

Objective

To evaluate whether garlicin can prevent reperfusion no-reflow in a catheter-based porcine model of acute myocardial infarction (AMI).

Methods

Twenty-two male Chinese mini swines were randomized into 3 groups: sham-operation group (n=6), control group (n=8), and garlicin group (n=8). The distal part of left anterior descending coronary artery (LAD) in swines of the latter two groups was completely occluded by dilated balloon for 2 h and a successful AMI model was confirmed by coronary angiography (CAG) and electrocardiograph (ECG), which was then reperfused for 3 h. In the sham-operation group, balloon was placed in LAD without dilatation. Garlicin at a dosage of 1.88 mg/kg was injected 10 min before LAD occlusion until reperfusion for 1 h in the garlicin group. To assess serial cardiac function, hemodynamic data were examined by catheter method before AMI, 2 h after occlusion and 1, 2, and 3 h after reperfusion. Myocardial contrast echocardiography (MCE) and double staining with Evans blue and thioflavin-S were performed to evaluate myocardial no-reflow area (NRA) and risk area (RA).

Results

Left ventricular systolic pressure and left ventricular end-diastolic pressure significantly improved in the garlicin group after reperfusion compared with the control group P<0.05) and 2 h after AMI (P<0.05). MCE showed garlicin decreased reperfusion NRA after AMI compared with the control group (P <0.05). In double staining, NRA/RA in the garlicin group was 18.78%, significantly lower than that of the control group (49.84%, P<0.01).

Conclusions

Garlicin has a preventive effect on the porcine model of myocardial infarction reperfusion no-reflow by improving hemodynamics and decreasing NRA.

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Authors and Affiliations

  1. Department of Cardiology, China-Japan Friendship Hospital, Beijing, 100029, China

    Jia-hui Li  (李佳慧), Peng Yang  (杨 鹏), Ai-li Li  (李爱莉), Yong Wang  (王 勇), Yuan-nan Ke  (柯元南) & Xian-lun Li  (李宪伦)

  2. Department of Integrative Cardiology, China-Japan Friendship Hospital, Beijing, 100029, China

    Zai-xiang Shi  (史载祥)

Authors
  1. Jia-hui Li  (李佳慧)
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  2. Peng Yang  (杨 鹏)
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  3. Ai-li Li  (李爱莉)
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  4. Yong Wang  (王 勇)
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  5. Zai-xiang Shi  (史载祥)
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  6. Yuan-nan Ke  (柯元南)
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  7. Xian-lun Li  (李宪伦)
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Corresponding author

Correspondence to Xian-lun Li  (李宪伦).

Additional information

Supported by Capital Medical Development Research Fund of China (No. 03III02)

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Li, Jh., Yang, P., Li, Al. et al. The preventive effect of garlicin on a porcine model of myocardial infarction reperfusion no-reflow. Chin. J. Integr. Med. 20, 425–429 (2014). https://doi.org/10.1007/s11655-012-1091-1

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  • DOI: https://doi.org/10.1007/s11655-012-1091-1

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