Abstract
Objective
To investigate the effects of drug-containing serum of Chinese herbal compound, Xiongshao Capsule (芎芍胶囊, XS, for activating-blood) and Huanglian Capsule (黄连胶囊, HL, for dispellingtoxin) on the oxidized low-density lipoprotein (ox-LDL)-induced inflammatory factors in human umbilical vein endothelial cells (HUVECs).
Methods
Thirty-two rats were randomly divided into four groups: the blank control group treated with distilled water, the positive control group treated with simvastatin (1.8 mg/kg), the test group I treated with Chinese herbal compound of XS (0.135 g/kg), and the test group II treated with Chinese herbal compound of XS (0.135 g/kg) and HL (0.135 g/kg). All the treatments were administered for 7 successive days by gastrogavage. Rats’ blood serum was harvested 1 h after the last administration to prepare respective drugcontaining serum. HUVECs were exposed to ox-LDL (100 μg/mL) to induce cell injury model and incubated with corresponding drug-containing serum for 24 h. Untreated HUVECs were set for blank control. Levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and soluble intercellular adhesion molecule-1 (sICAM-1) in supernatant of cultured HUVECs were determined by enzyme-linked immunosorbent assay (ELISA). HUVEC surface expressions of ICAM-1 and E-selectin were determined by flow cytometry.
Results
Levels of IL-6, TNF-α, and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation (P<0.01), while the abnormal elevations, except sICAM-1 in the test group I, were all reduced in the treated groups (the positive control and the two test groups) significantly (P<0.01 or P<0.05). Besides, the effect in the test group II seemed somewhat higher than that in the test group I but with no statistical significance (P>0.05).
Conclusion
Drug-containing serum of XS plus HL has a certain inhibitory effect on the vascular endothelial inflammation response induced by ox-LDL.
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References
Shi DZ, Xu H, Yin HJ, Zhang JC, Chen KJ. Combination and transformation of toxin and blood stasis in etiopathogenesis of thrombotic cerebrocardiovascular diseases. J Chin Integr Med (Chin) 2008;6:1105–1108.
Wen C, Xu H, Huang QF, Chen KJ. Effect of drugs for promoting blood circulation on blood lipids and inflammatory reaction of atherosclerotic plaques in apoE gene deficiency mice. Chin J Integr Tradit West Med (Chin) 2005;25:345.
Zhang JC, Chen KJ, Zheng GJ, Zhang WG, Shi DZ, Yin HJ, et al. Regulatory effect of Chinese herbal compound for detoxifying and activating blood circulation on expression of NF-κB and MMP-9 in aorta of apolipoprotein E gene knocked-out mice. Chin J Integr Tradit West Med (Chin) 2007;27:40–44.
Jiang YR, Chen KJ, Xu YG, Yang XH, Yin HJ. Effects of Propyl Gallate on adhesion of polymorphonuclear leukocytes to human endothelial cells induced by tumor necrosis factor alpha. Chin J Integr Med 2009;15:47–53.
Ou HC, Lee WJ, Lee IT, Chiu TH, Tsai KL, Lin CY, et al. Ginkgo biloba extract attenuates oxLDL-induced oxidative functional damages in endothelial cells. J Appl Physiol 2009;106:1674–1685.
Landsberger M, Wolff B, Jantzen F, Rosenstengel C, Vogelgesang D, Staudt A, et al. Cerivastatin reduces cytokineinduced surface expression of ICAM-1 via increased shedding in human endothelial cells. Atherosclerosis 2007;190:43–52.
Xenos ES, Stevens SL, Freeman MB, Cassada DC, Goldman MH. Nitric oxide mediates the effect of fluvastatin on intercellular adhesion molecule-1 and platelet endothelial cell adhesion molecule-1 expression on human endothelial cells. Ann Vasc Surg 2005;19:386–392.
Hopkins AM, Baird AW, Nusrat A. ICAM-1: targeted docking for exogenous as well as endogenous ligands. Adv Drug Deliv Rev 2004;56:763–778.
Kaski JC, Fernández-Bergés DJ, Consuegra-Sánchez L, Fernández JM, García-Moll X, Mostaza JM, et al. A comparative study of biomarkers for risk prediction in acute coronary syndrome—results of the SIESTA (Systemic Inflammation Evaluation in non-ST-elevation Acute coronary syndrome) study. Atherosclerosis 2010;212:636–643.
Zhang JC, Chen KJ. Theoretical thinking on relationship between toxic-stasis pathogenicity and atherosclerotic vulnerable plaque. Chin J Integr Tradit Westn Med (Chin) 2008;28:366–368.
Lu XH, Ding SW. Clinical effect and mechanism on treating unstable angina pectoris by Huanglian Jiedu Capsule. J Shandong Univ Tradit Chin Med (Chin) 2005;29:457–460.
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Supported by the National Developing Plan for Basic Key Items (973 Plan, No. 2006CB504803)
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Jiang, Yr., Miao, Y., Yang, L. et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells. Chin. J. Integr. Med. 18, 30–33 (2012). https://doi.org/10.1007/s11655-011-0849-1
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DOI: https://doi.org/10.1007/s11655-011-0849-1