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Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway

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Abstract

Objective

To investigate effects of Dahuang Zhechong Pill (大黄{ie371-1}虫丸, DHZCP) on the cell cycle and the related signal pathways in vascular smooth muscle cells (VSMCs) stimulated by platelet-derived growth factor (PDGF) with the method of serum pharmacology.

Methods

DNA synthesis in VSMCs was examined by detecting 5′-bromo-2′-deoxyuridine incorporation with the immunocytochemical method. The cycle of VSMCs was evaluated with flow cytometry. Expressions of cyclin D1, p27, protein kinase Cα (PKCα), and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) were quantified by Western blot method.

Results

DHZCP containing serum significantly inhibited DNA synthesis of PDGF-stimulated VSMCs, arrested the cells in G G1 phase, modulated the protein expressions of cyclin D D1 and p27, and suppressed the activation of PKCα and ERK1/2.

Conclusion

DHZCP containing serum inhibits VSMCs proliferation via modulating the expressions of cell cycle proteins to arrest the cell in G G1 phase, which is attributed to, at least in part, suppressing PKCα-ERK1/2 signaling in VSMCs.

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Authors and Affiliations

  1. Department of Pharmacology, Xi’an Jiaotong University School of Medicine, Xi’an, 710061, China

    Na Liu  (刘 娜), Jun-tian Liu  (刘俊田) & Pei-pei Lu  (路培培)

  2. Department of Pharmacy, the Second Affiliated Hospital, Xi’an Jiaotong University School of Medicine, Xi’an, 710004, China

    Na Liu  (刘 娜)

  3. Scientific Research and Experiment Center, the Second Affiliated Hospital, Xi’an Jiaotong University School of Medicine, Xi’an, 710004, China

    Yuan-yuan Ji  (姬媛媛)

Authors
  1. Na Liu  (刘 娜)
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  2. Jun-tian Liu  (刘俊田)
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  3. Yuan-yuan Ji  (姬媛媛)
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  4. Pei-pei Lu  (路培培)
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Correspondence to Jun-tian Liu  (刘俊田).

Additional information

Supported by the National Natural Science Foundation of China (No. 30572347)

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Liu, N., Liu, Jt., Ji, Yy. et al. Dahuang Zhechong Pill (大黄虫丸) containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-Extracellular regulated kinase 1/2 signaling pathway. Chin. J. Integr. Med. 18, 371–377 (2012). https://doi.org/10.1007/s11655-011-0696-0

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  • DOI: https://doi.org/10.1007/s11655-011-0696-0

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