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Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549

Chinese Journal of Integrative Medicine volume 17, pages 218–223 (2011)Cite this article

Abstract

Objective

To observe the proliferation inhibition, apoptosis, and cell proliferation cycle of human lung carcinoma cell line A549 treated with Inotodiol extracts from Inonotus obliquus and explore the possibility of Inotodiol extracts from Inonotus obliquus as a new tumor chemopreventive drug.

Methods

Human lung cancer cell line A549 was treated with different concentrations of Inotodiol, the effects of Inotodiol on cell apoptosis, the expression of Ki-67, Bcl-2, Bax, and p53 and cell cycle were detected by TUNEL assay, immunohistochemistry, and flow cytometry assay respectively.

Results

Inotodiol extracts had antiproliferation effect on human lung carcinoma cell line A549. The expression of Ki-67 decreased with the increase of Inotodiol concentration and exposure time (P<0.05), in a dose-dependent and time-dependent manner. The typical characteristics of the apoptosis of A549 cells treated with Inotodiol were observed, and the apoptotic rate of A549 cell at 48 h was the highest by TUNEL assay. Inotodiol arrested A549 cells in the S phase, and apoptotic peak was observed by flow cytometry. Immunocytochemistry indicated that the expression of Bcl-2 protein decreased, while the expression of p53 and Bax proteins increased in A549 cells treated with Inotodiol, compared with the control cells (P<0.05).

Conclusion

Inotodiol can inhibit proliferation and induce the apoptosis of A549 cells, and its molecular mechanism may be associated with the up-regulating expression of p53 and bax proteins and down-regulating expression of Bcl-2 protein, which arrested A549 cells in S phase.

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Authors and Affiliations

  1. Department of Pathology, Jilin Medical College, Jilin, 132013, China

    Xiu-hong Zhong  (钟秀宏)

  2. Department of Pathology, Jilin City People’s Hospital, Jilin, 132001, China

    Li-bo Wang  (王立波)

  3. Department of Pathology and Forensic Medicine, Basic Medical College, Yanbian University Health Science Center, Yanji, 133000, Jilin, China

    Dong-zhi Sun  (孙东植)

Authors
  1. Xiu-hong Zhong  (钟秀宏)
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  2. Li-bo Wang  (王立波)
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  3. Dong-zhi Sun  (孙东植)
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Corresponding author

Correspondence to Xiu-hong Zhong  (钟秀宏).

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Zhong, Xh., Wang, Lb. & Sun, Dz. Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549. Chin. J. Integr. Med. 17, 218–223 (2011). https://doi.org/10.1007/s11655-011-0670-x

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  • DOI: https://doi.org/10.1007/s11655-011-0670-x

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