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Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums

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Abstract

Objective

To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang (丹瓜方), a Chinesea Chinese), a Chinese herbal compound prescription on endothelial cells of vein (ECV304) cultivated in mediums of different glucose concentrations as well as the proliferation of those cells in the same conditions, in order to reveal the value of Dan Gua-Fang in preventing and treating endothelial damage caused by hyperglycemia in diabetes mellitus.

Methods

The research was designed as three stages. The growing state and morphological changes were observed when ECV304 were cultivated in the culture mediums, which have different glucose concentrations with or without Dan Gua-Fang and at the same time with or without colchicine.

Results

(1) Dan Gua-Fang at all concentrations reduced the floating cell population of ECV304 cultivated in hyperglycemia mediums. (2) Dan Gua-Fang at all concentrations and hyperglycemia both had a function of promoting “pseudopod-like” structure formation in cultivated ECV304, but the function was not superimposed in mediums containing both hyperglycemia and Dan Gua-Fang. (3) Colchicine reduced and even vanished the “pseudopod-like” structure of the endotheliocyte apparently cultivated in mediums of hyperglycemia or with Dan Gua-Fang. The “pseudopod-like” structure of the endotheliocyte emerged quickly in Dan Gua-Fang groups after colchicine was removed, but it was not the case in hyperglycemia only without Dan Gua-Fang groups. (4) Dan Gua-Fang reduced the mortality of cells cultivated in mediums containing colchicine. The cell revived to its normal state fast after colchicine was removed.

Conclusion

Dan Gua-Fang has the functions of promoting the formation of cytoskeleton and fighting against colchicine cytotoxicity.

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Authors and Affiliations

  1. Department of Endocrinology, People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, 350004, China

    Xian-pei Heng  (衡先培), Wei-dong He  (何卫东), Ke-dan Chu  (褚克丹), Hai-xia Zheng  (郑海霞), Liu-qing Yang  (杨柳清) & Fang Guo  (郭 芳)

  2. China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Xian-pei Heng  (衡先培) & Ke-ji Chen  (陈可冀)

  3. Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China

    Zhen-feng Hong  (洪振丰) & Ling Chen  (陈 玲)

  4. Fujian Institute of Integrative Chinese and Western Medicine, Fuzhou, 350108, China

    Ke-ji Chen  (陈可冀) & Wen-lie Chen  (陈文列)

Authors
  1. Xian-pei Heng  (衡先培)
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  2. Ke-ji Chen  (陈可冀)
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  3. Zhen-feng Hong  (洪振丰)
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  4. Wei-dong He  (何卫东)
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  5. Ke-dan Chu  (褚克丹)
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  6. Wen-lie Chen  (陈文列)
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  7. Hai-xia Zheng  (郑海霞)
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  8. Liu-qing Yang  (杨柳清)
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  9. Ling Chen  (陈 玲)
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  10. Fang Guo  (郭 芳)
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Corresponding author

Correspondence to Xian-pei Heng  (衡先培).

Additional information

Supported by the China Postdoctoral Science Foundation (No. 20070410128), CHEN Ke-ji Development Fund of Integrative Medicine (No. CKJ2006001) and Science Foundation of Health Department, Fujian Province (No. WZY0618)

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Heng, Xp., Chen, Kj., Hong, Zf. et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums. Chin. J. Integr. Med. 17, 126–133 (2011). https://doi.org/10.1007/s11655-011-0646-X

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  • DOI: https://doi.org/10.1007/s11655-011-0646-X

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