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Effect of Bushen Huoxue Decoction (补肾活血饮) on the orphan receptor and tyrosine hydroxylase in the brain of rats with Parkinson’s disease

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Abstract

Objective

To explore the effect of Bushen Huoxue Decoction (补肾活血饮, BHD) on the orphan receptor (Nurr1) and tyrosine hydroxylase (TH) in the brain of rats with Parkinson’s disease (PD).

Methods

One hundred and twenty SD rats were divided into 100 in the model group and 20 in the normal control group, fififtyeight SD rats from the model group, established into PD model successfully by injuring their substantia nigra (SSN) with 6-hydroxydopamine, were divided equally into the model group and the test group, and they were treated with saline and BHD, respectively, for eight successive weeks. The change in the rats’ behavior before and after treatment was observed by counting the cycles of rotation induced by apomorphine injection; the pathology of neurons, level of Nurr1 mRNA expression, and amount of TH positive cells in SSN were observed after treatment.

Results

The rats’ behavior was improved in the tested group signifificantly, the rotation cycle after treatment being 84.0±20.0 cycles/40 min, which was signifificantly lower than that in the model group (377.0 ±62.3 cycles/40 min, P<0.01). Besides, the Nurr1 mRNA expression and TH positive cell in the test group were 0.97±0.15 and 49.40±14.72, respectively, which were signifificantly higher than those in the model group, 0.22±0.03 and 5.45±2.58, respectively (all P<0.01).

Conclusion

BHD could treat PD by enhancing the Nurr1 mRNA expression, increasing the TH content in brain, and promoting the repairing of injured neuron in cerebral SSN.

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Authors and Affiliations

  1. Institute of Traditional Chinese Medicine, General Hospital of PLA, Beijing, 100853, China

    Ming-hui Yang  (杨明会), Hai-ming Wang  (王海明) & Yi Liu  (刘 毅)

Authors
  1. Ming-hui Yang  (杨明会)
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  2. Hai-ming Wang  (王海明)
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  3. Yi Liu  (刘 毅)
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Corresponding author

Correspondence to Ming-hui Yang  (杨明会).

Additional information

Supported by National Natural Science Foundation of China (No. 30672762)

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Yang, Mh., Wang, Hm. & Liu, Y. Effect of Bushen Huoxue Decoction (补肾活血饮) on the orphan receptor and tyrosine hydroxylase in the brain of rats with Parkinson’s disease. Chin. J. Integr. Med. 17, 43–47 (2011). https://doi.org/10.1007/s11655-011-0618-1

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  • DOI: https://doi.org/10.1007/s11655-011-0618-1

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