Effects of Yinian Jiangya Decoction (颐年降压饮) containing serum on cytokines secretion of vascular endothelium of spontaneously hypertensive rats

Abstract

Objective

To observe the effects of Yinian Jiangya Decoction (颐年降压饮, YNJYD) on cytokine secretion in spontaneoulsy hypertensive rats (SHRs) vascular endothelium.

Methods

Aortic endothelial cells (ECs) were primarily cultured from SHRs; male SD rats were treated with different doses (high, medium, and low doses) of YNJYD, the blood was collected on the 21st day, and then, the serum was separated. ECs were cocultured with the serum for different time courses, and the culture supernatant concentrations of endothelin (ET)-1, nitric oxide (NO), tissue-type plasminogen activator (t-PA), and plasminogen activator inhibitor (PAI-1) were determined by ABC-ELISA methods.

Results

ET-1, NO, t-PA, and PAI-1 levels in endothelial cell culture supernatant were increased in a time-dependent manner; YNJYD could significantly elevate NO and t-PA expressions in ECs, while ET-1 and PAI-1 expressions were dramatically decreased; these effects of YNJYD were in a concentration-dependent manner.

Conclusion

The therapeutic effect of YNJYD on hypertension is attributed to its effect on regulating vessel dilation and blood coagulation, in which ET-1/NO and PAI-1/t-PA are two pairs of pivotal mediators.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Zhao YH, Liu YD, Huang PD, Xiang P. Effects of clinical treatment and protection on endothelial function of early essential hypertension’s patients treated with YNJY soup in southern Guangdong area. China J Tradit Chin Med Pharm (Chin) 2009;24:1149–1152.

    CAS  Google Scholar 

  2. 2.

    Zhang YH, Hu Y, Mei H, Wang HF, Guo T, Wei WN. Compared study for three methods to isolate endothelial cells from rat aorta. Chin J Microcirc (Chin) 2007;17:15–16.

    CAS  Google Scholar 

  3. 3.

    Hynynen MM, Khalil RA. The vascular endothelin system in hypertension—recent patents and discoveries. Recent Pat Cardiovasc Drug Discov 2006;1:95–108.

    Article  CAS  PubMed  Google Scholar 

  4. 4.

    Spieker LE, Noll G, Ruschitzka FT, Lüscher TF. Endothelin receptor antagonists in congestive heart failure: a new therapeutic principle for the future? J Am Coll Cardiol 2001;37:1493–505.

    Article  CAS  PubMed  Google Scholar 

  5. 5.

    Giles TD. Aspects of nitric oxide in health and disease: a focus on hypertension and cardiovascular disease. J Clin Hypertens (Greenwich) 2006;8:2–16.

    CAS  Google Scholar 

  6. 6.

    Augustyniak RA, Thomas GD, Victor RG, Zhang W. Nitric oxide pathway as new drug targets for refractory hypertension. Curr Pharm Des 2005;11:3307–3315.

    Article  CAS  PubMed  Google Scholar 

  7. 7.

    Siragy HM. Improving vascular function in hypertension: potential benefits of combination therapy with amlodipine and renin-angiotensin-aldosterone system blockers. J Hypertens 2010;28:2–8.

    Article  CAS  PubMed  Google Scholar 

  8. 8.

    Tabak O, Gelisgen R, Uzun H, Kalender B, Balci H, Curgunlu A, et al. Hypertension and hemostatic/fibrinolytic balance disorders. Clin Invest Med 2009;32:E285.

    CAS  PubMed  Google Scholar 

  9. 9.

    Fogari R, Zoppi A. Antihypertensive drugs and fibrinolytic function. Am J Hypertens 2006;19:1293–1299.

    Article  CAS  PubMed  Google Scholar 

  10. 10.

    Ulfhammer E, Ridderstrale W, Andersson M, Karlsson L, Hrafnkelsdóttir T, Jern S. Prolonged cyclic strain impairs the fibrinolytic system in cultured vascular endothelial cells. J Hypertens 2005;23:1551–1557.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Yong-hua Zhao 赵永华.

Additional information

Supported by Science and Technology Development Fund of Macao Special Administrative Region (No.105/2005/A)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zhao, Y., Xu, Y., Guan, Y. et al. Effects of Yinian Jiangya Decoction (颐年降压饮) containing serum on cytokines secretion of vascular endothelium of spontaneously hypertensive rats. Chin. J. Integr. Med. 16, 344–347 (2010). https://doi.org/10.1007/s11655-010-0524-y

Download citation

Keywords

  • Yinian Jiangya Decoction
  • endothelial cell
  • cytokine