Abstract
Objective
To study the effects of Erlong Zuoci Pill (耳聋左慈丸, ELZCP) and its disassembled: prescriptions on gentamicin (GM)-induced ototoxicity model in vitro.
Methods
After the spiral organ of cochleae: of newborn mice (postnatal days: 2–3) cultured for 24 h, GM alone or combined with water extracting-alcohol precipitating solution of ELZCP or with its disassembled prescriptions was added. Hair cells were observed under a fluorescence microscope after TRITC-phalloidin staining, and the cochlear hair cell loss rate was calculated by counting the whole cochlear hair cells and analyzed by whole cochlear hair cells analyzing software.
Results
GM induced cochlear outer hair cells (OHCs) and inner hair cells (IHCs) injuries in a dose-dependent manner, and they were significantly different as compared with those in the normal control group (P<0.05, P<0.01). ELZCP at the concentration of 0.003–3 mg/mL could decrease the hair cells loss induced by the 0.3 mmol/L GM (P<0.05, P<0.01), the effects was in a dose-dependent manner, and the concentration of 0.3 mg/mL showed the optimal protective effect. For the ELZCP disassembled prescriptions, Liuwei-Dihuang could decrease OHC loss rate than that in the 0.3 mmol/L GM model group (P<0.05), but the OHC loss rate was still higher than that in the ELZCP group (P<0.01), which indicated that the protective effect of hair cells by Liuwei-Dihuang was not better than that of ELZCP. Poria decreased OHC loss rate from 72.1 % ±3.7 % to 58.8 %± 8.2 % (P<0.05).
Conclusions
ELZCP could play a role in antagonizing the injury of cochlear hair cells induced by GM ototoxicity,: and its disassembled prescriptions, Liuwei-Dihuang was the main component to protect the cochlear hair cells from GM-induced ototoxicity, and Magnetitum combined with Radix Bupleurui could strengthen the action of the whole prescription; Poria could reduce GM-induced OHC loss.
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References
McFadden SL, Ding D, Salvemini D, Salvi RJ. M40403, a superoxide dismutase mimetic, protects cochlear hair cells from gentamicin, but not cisplatin toxicity. Toxicol Appl Pharmacol 2003;186:46–54.
Qi W, Ding D, Salvi RJ. Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures. Hear Res 2008;236:52–60.
Chen FQ, Schacht J, Sha SH. Aminoglycoside-induced histone deacetylation and hair cell death in the mouse cochlea. J Neurochem 2009;108:1226–1236.
Dong Y, Shi JR, Wang J, Han ZF, Guo RX. Effects of puerarin on the gentamicin-induced cochlear hair cells damage. Otolarngol Integr Tradit West Med (Chin) 2008;16:401–403.
Rui QG. The effects of modified Erlong-Zuoci-Wan on treating 86 cases of tinnitus and hearing loss with Kidney deficiency. Clin J Tradit Chin Med (Chin) 2007;19: 280–281.
Li TD, Shi YZ, Shi XJ, Zhang Y, Zhang XM, et al. Prevention and mechanism of modified Erlong- Zuoci-Wan on gentamicin ototoxcity. Chin J Clin Rehabil (Chin) 2004;8:6150–6151.
Qiu F, Liu J, Kang SJ, Shi YZ, Shi XJ, Zhang Y. The protective effects of modified Erlong-Zuoci-Wan on cochlear succinate dehydrogenase. Chin J New Drug (Chin) 2004;13:991–993.
The State Pharmacopoeia Committee. Chinese pharmacopoeia. Beijing: Chemical Industry Publishing House; 2005:430.
Liu YC, Liu GY, Liu RL, Cheng XZ, Li L, Hou JP. Effects of poria cocos on ototoxicity induced by kanamycin in guinea pigs. Chin J Integr Tradit West Med (Chin) 1995;15:422–423.
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Supported by Shanghai Science and Technology Commission (No. 04ZR14130) and Leading Academic Discipline Project of Shanghai Municipal Education Commission (No. J50301).
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Dong, Y., Cao, By., Wang, J. et al. Effects of Erlong Zuoci Pill (耳聋左慈丸) and its disassembled prescriptions on gentamicin-induced ototoxicity model in vitro . Chin. J. Integr. Med. 16, 258–263 (2010). https://doi.org/10.1007/s11655-010-0258-x
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DOI: https://doi.org/10.1007/s11655-010-0258-x