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The effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on in vitro endothelial progenitor cell tube formation

Chinese Journal of Integrative Medicine volume 16, pages 50–53 (2010)Cite this article

Abstract

Objective

To observe the effect of Xuefu Zhuyu Decoction (血府逐瘀汤)-containing serum (XFZYD-CS) on endothelial progenitor cell (EPC) tube formation in vitro.

Methods

Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge. EPCs were separated by the differential attachment method, and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation.

Results

After one day, EPCs exposed to the serum containing 5%, 10% and 15% XFZYD-CS formed typical tubes or vessel networks. The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group.

Conclusion

XFZYD-CS could induce EPC angiogenesis and hasten tube formation, especially in capillary vessels. The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.

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Author information

Affiliations

  1. Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China

    Dong Gao, Li-ya Wu, Yu-huan Jiao & Wen-yuan Chen

  2. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Yan Chen, Bo Lu & Jun Song

  3. Harvard Medical School, Boston, MA, 02215, USA

    Ted J. Kaptchuk

  4. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Ke-ji Chen

Authors
  1. Dong Gao
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  2. Li-ya Wu
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  3. Yu-huan Jiao
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  4. Wen-yuan Chen
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  5. Yan Chen
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  6. Ted J. Kaptchuk
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  7. Bo Lu
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  8. Jun Song
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  9. Ke-ji Chen
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Corresponding author

Correspondence to Jun Song.

Additional information

Supported by the National Natural Science Foundation of China (No. 30772877); Basic Research Fund of China Academy of Chinese Medical Sciences (No. ZZ2006039); Fujian Academy of Integrative Medicine Foundation (No. 3000-905010805)

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Gao, D., Wu, Ly., Jiao, Yh. et al. The effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on in vitro endothelial progenitor cell tube formation. Chin. J. Integr. Med. 16, 50–53 (2010). https://doi.org/10.1007/s11655-010-0050-y

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  • DOI: https://doi.org/10.1007/s11655-010-0050-y

Keywords

  • Xuefu Zhuyu Decoction
  • endothelial progenitor cells
  • tube formation