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Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats

  • Experimental Research
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Abstract

Objective

To study the effects and possible mechanisms of effective composite of Naodesheng (脑得生, NDS) on permanent cerebral ischemia-induced injury in rats.

Methods

Male Sprague-Dawley rats: with middle cerebral artery occlusion (MCAO) were established with the modified suture method, and they were randomly divided into the following groups: the sham-operated group, the model group, the Nimodipine group (0.012 g/kg), the NDS group (1.075 g/kg), the total extracts group (0.23 g/kg), the high-dose NEC group (0.07 g/kg), the middle-dose NEC group (0.02 g/kg), and the low-dose NEC group (0.007 g/kg). The aforesaid medicines were administered at the 2nd, 4th, and 24th h after focal cerebral ischemia, and the infarction size and water content in the brain were evaluated at the 26th h after MCAO. Then, after oral administration once daily for 7 successive days, the changes in the degree of neurological deficit, oxidative stress, and apoptosis were measured on the 7th day.

Results

NEC could significantly reduce the infarction size after focal cerebral ischemia, and slightly relieve water content in the brain, significantly alleviate neurological function impairment, increase the levels of superoxide dismutase (SOD) and adenosine triphosphate enzyme (ATPase) activity, and decrease the content of malondialdehyde (MDA). NEC could also extenuate Bax and caspase-3 expression in the hippocampus tissue of the ischemic region. As compared with the three NEC treated groups, the high-dose NEC showed better efficacy.

Conclusions

NEC could significantly reduce brain injury induced by ischemia;: its mechanism was closely associated with hindering oxidative stress and apoptosis. The effective composite-compositeguided methodology is a feasible tool to improve the neuro-protective properties of the Chinese medicine guided prescription NDS against focal cerebral ischemia in rats.

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Authors and Affiliations

  1. National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100050, China

    Li Zhang  (张莉), Xin-rui Cheng  (程新锐), Ruo-yun Chen  (陈若芸), Xiao-ming Zhu  (竺晓鸣) & Guan-hua Du  (杜冠华)

  2. Department of Pharmacology, Institute of Basic Medical Science, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100055, China

    Xin-rui Cheng  (程新锐)

Authors
  1. Li Zhang  (张莉)
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  2. Xin-rui Cheng  (程新锐)
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  3. Ruo-yun Chen  (陈若芸)
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  4. Xiao-ming Zhu  (竺晓鸣)
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  5. Guan-hua Du  (杜冠华)
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Corresponding author

Correspondence to Guan-hua Du  (杜冠华).

Additional information

Supported by Chinese Herbs Special Project of State Administration of Traditional Chinese Medicine (No. 02-03-ZP08); National High Technology Research and Development Program of China, the Fund of Research and Application of Screening Technology Platform of Innovative Drug (863 Program, No. 2004AA2Z3782)

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Zhang, L., Cheng, Xr., Chen, Ry. et al. Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats. Chin. J. Integr. Med. 15, 377–383 (2009). https://doi.org/10.1007/s11655-009-0377-4

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  • DOI: https://doi.org/10.1007/s11655-009-0377-4

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