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Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model

  • Experimental Research
  • Published:
Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the inhibitory effects of Kangjia Pill (抗甲丸, KJP) on the cell proliferation in rat goiter model induced by methimazole (MMI).

Methods

Fifty-six Wistar rats were randomly divided into four groups: the normal group, MMI model group (MMI), low dose of KJP group (LKJP), and high dose of KJP (HKJP). Except the normal group (20 rats), the other groups (12 rats in each) were given 0.04% (w/v) MMI through the drinking water until the end of the experiment. One week later, the rats in the LKJP and HKJP groups were given KJP by gastrogavage at the dose of 250 mg/(kg · d) and 1 000 mg/(kg · d), respectively for 12 weeks. The relative thyroid weight (mg/100 g body weight) of each rat was accessed. The expression of proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry, and the correlation analysis between the PCNA positive thyrocytes and the relative thyroid weight was performed. The expressions of PCNA and cyclin D1 were examined with Western blotting.

Results

After KJP treatment for 12 weeks, compared with the MMI group, the relative thyroid weight of the HKJP group decreased significantly, and the positive thyrocyte populations of PCNA in the two KJP groups reduced markedly (all P<0.05). The correlation analysis showed that PCNA was closely correlated with thyrocyte proliferation (r=0.685, P<0.05). KJP significantly decreased the protein expression of PCNA and cyclin D1 in the thyroid specimens (P<0.05), the high dose showed better effects.

Conclusion

KJP played a therapeutic role via inhibiting cell proliferation in the rat goitrous glands.

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Authors and Affiliations

  1. Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China

    Yong Han  (韩 勇), Hai-qing Zhang  (张海清) & Jia-jun Zhao  (赵家军)

  2. Department of Endocrinology of Zaozhuang City Hospital, Shandong, 277102, China

    Jing Zhou  (周 静) & Shu-jing Yu  (于淑静)

  3. Scientific Centre, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China

    Bin Cui  (崔 彬) & Ling Gao  (高 聆)

Authors
  1. Yong Han  (韩 勇)
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  2. Jing Zhou  (周 静)
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  3. Shu-jing Yu  (于淑静)
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  4. Bin Cui  (崔 彬)
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  5. Hai-qing Zhang  (张海清)
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  6. Ling Gao  (高 聆)
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  7. Jia-jun Zhao  (赵家军)
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Corresponding author

Correspondence to Jia-jun Zhao  (赵家军).

Additional information

Supported by the National Natural Science Foundation of China (No. 30672748), the Natural Science Foundation of Shandong Province (No. 22003C02), and the Shandong Administration of Traditional Chinese Medicine (No. 2005-070)

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Han, Y., Zhou, J., Yu, Sj. et al. Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model. Chin. J. Integr. Med. 15, 284–288 (2009). https://doi.org/10.1007/s11655-009-0284-8

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  • DOI: https://doi.org/10.1007/s11655-009-0284-8

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