Abstract
Objective
To observe the effects of sodium tanshinone II A sulfonate (STS) on angiotensin II (Ang II)-induced hypertrophy of myocardial cells through the expression of phosphorylated extracellular signal-regulated kinase (p-ERK1/2).
Methods
In the primary culture of neonatal rat myocardial cells, the total protein content in myocardial cells was determined by coomassie brilliant blue and the protein synthesis rate was measured by [3H]-Leucine incorporation as indexes for hypertrophy of myocardial cells. The expression of p-ERK1/2 was determined using Western blot and immunofluorescence labeling.
Results
(1) The total protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial cells were stimulated by Ang II (1 μmol/L) for 24 h; STS markedly inhibited the increment of the total protein level induced by Ang II and the syntheses of protein. (2) After pretreatment of myocardial cells with Ang II (1 μmol/L) for 5 min, the p-ERK1/2 protein expression was increased, with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with STS at different doses (2, 10, 50 μmol/L) for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated by Ang II in a dose-dependent manner. (3) After the myocardial cells were stimulated by Ang II (1 μmol/L), the immunofl uorescence of ERK1/2 rapidly appeared in the nucleus. The activation and translocation process of ERK1/2 induced by Ang II was blocked distinctly by STS.
Conclusion
STS inhibited the myocardial cell hypertrophy induced by Ang II, and the mechanism may be associated with the inhibition of p-ERK1/2 expression.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cohn JN, Ferrari R, Sharpe N. Cardiac remodeling-concepts and clinical implications: a consensus paper from an international forum on cardiac remodeling. Behalf on an international forum on cardiac remodeling. J Am Coll Cardiol 2000;35:569–582.
Liu F, Zheng Z. The mechanisms of radix salviae miltiorrhizae in prevention of left ventricular hypertrophy in spontaneously hypertensive rats. Chin J Emerg Med (Chin) 2004;13(5):322–324.
van Kesteren CA, van Heugten HA, Lamers JM, et al. Angiotensin II-mediated growth and antigrowth effects in cultured neonatal rat cardiac myocytes and fibroblasts. J Mol Cell Cardiol 1997;29:2147–2157.
Yamazaki T, Komuro I, Kudoh S, et al. Angiotensin II partly mediates mechanical stress-induced cardiac hypertrophy. Circ Res 1995;77:258–265.
Frey N, Olson EN. Cardiac hypertrophy: the good, the bad and the ugly. Annu Rev Physiol 2003;65:45–79.
Frey N, Katus HA, Olson EN, et al. Hypertrophy of the heart: a new therapeutic target? Circulation 2004;109:1580–1589.
Sugden PH. Signalling pathways in cardiac myocyte hypertrophy. Ann Med 2001;33:611–622.
Zhang SQ, Ding B, Guo ZG, et al. Inhibitory effect of antisense oligodeoxynucleotide to 44/p42 MAPK on angiotensin II-induced hypertrophic response in cultured neonatal rat cardiac myocyte. Acta Pharmacol Sin 2004;25(1):41–46.
Ouyang X, Takahashi K, Komatsu K, et al. Protective effect of Salvia miltiorrhiza on angiotensin II-induced hypertrophic responses in neonatal rat cardiac cells. Jpn J Pharmacol 2001;87:289–96.
Xie H, Zheng Z. The effect of tanshinon II A on hypertrophy and apoptosis of cardiomycytes induced by angiotensin II. Chin J Hypertens (Chin) 2004;12(4):359–361.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, Ss., Feng, J., Zheng, Z. et al. Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells. Chin. J. Integr. Med. 14, 123–127 (2008). https://doi.org/10.1007/s11655-008-0123-3
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11655-008-0123-3