Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma
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To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Th1) specific transcription factor T-box expressed in T cells (T-bet) expression and Th1/Th2 equilibrium.
The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon γ (IFN-γ) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4+CCR3+ and CD4+CCR5+ cells were counted by flow cytometry.
The expression of T-bet mRNA and the level of IFN-γ were lower, but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 μ g/ml) intervention, the former two parameters raised and showed a positive correlation between them, while the level of IL-4 was decreased. The mean percentage of CD4+CCR3+ cells in asthma patients was significantly higher but that of CD4+CCR5+ cells was lower when compared with those in healthy subjects respectively. After AM intervention, the abnormal change in the two indexes was improved to certain extent, showing a reversing status of Th2 polarization.
AM could increase the expression of T-bet mRNA and Th1 cytokines such as IFN-γ, and might reverse the Th2 predominant status in asthma patients.
Key Wordasthma Astragalus membranaceus transcription factor T-box expressed in T cells T helper cells Tinterferon-γ
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- 4.Liu CT, Wang G, Luo FM, et al. Imbalanced T cell-specific transcription factors T-bet and GATA-3 contributes to type 2 T helper cell polarization in asthmatic patients. Chin J Tuberc Respir Dis 2004;27(6): 398–402.Google Scholar
- 7.Global Initiative for Asthma (GINA). Global strategy for asthma management and prevention: NHLBI/WHO Workshop report. Bethesda: National Heart, Lung and Blood Institute. National Institute of Health, 2002.Google Scholar
- 15.Mao SP, Cheng KL, Zhou YF. Modulatory effect of Astragalus membranaceus on Th1/Th2 cytokine in patients with herpes simplex keratitis. Chin J Integr Tradit West Med 2004;24(2): 121–123.Google Scholar
- 16.World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA 2000;284(23): 3043–3045.Google Scholar