Advertisement

Chinese Journal of Integrative Medicine

, Volume 12, Issue 4, pp 262–267 | Cite as

Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

  • Wang Gang  (王刚)
  • Liu Chun-tao  (刘春涛)Email author
  • Wang Zeng-li  (王曾礼)
  • Yan Cun-liang  (颜存粮)
  • Luo Feng-ming  (罗凤鸣)
  • Wang Lei  (王蕾)
  • Li Ting-qian  (李廷谦)
Article

Abstract

Objective

To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Th1) specific transcription factor T-box expressed in T cells (T-bet) expression and Th1/Th2 equilibrium.

Methods

The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon γ (IFN-γ) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4+CCR3+ and CD4+CCR5+ cells were counted by flow cytometry.

Results

The expression of T-bet mRNA and the level of IFN-γ were lower, but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 μ g/ml) intervention, the former two parameters raised and showed a positive correlation between them, while the level of IL-4 was decreased. The mean percentage of CD4+CCR3+ cells in asthma patients was significantly higher but that of CD4+CCR5+ cells was lower when compared with those in healthy subjects respectively. After AM intervention, the abnormal change in the two indexes was improved to certain extent, showing a reversing status of Th2 polarization.

Conclusion

AM could increase the expression of T-bet mRNA and Th1 cytokines such as IFN-γ, and might reverse the Th2 predominant status in asthma patients.

Key Word

asthma Astragalus membranaceus transcription factor T-box expressed in T cells T helper cells Tinterferon-γ 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Holgate ST. The epidemic of allergy and asthma. Nature 1999;402(Suppl): B2–B4.PubMedCrossRefGoogle Scholar
  2. 2.
    Kon OM, AB Kay. I cells and chronic asthma. Int Arch Allergy Immunol 1999;118(2–4): 133–135.PubMedCrossRefGoogle Scholar
  3. 3.
    Szabo SJ, Kim ST, Costa GL, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 2000;100(6): 655–669.PubMedCrossRefGoogle Scholar
  4. 4.
    Liu CT, Wang G, Luo FM, et al. Imbalanced T cell-specific transcription factors T-bet and GATA-3 contributes to type 2 T helper cell polarization in asthmatic patients. Chin J Tuberc Respir Dis 2004;27(6): 398–402.Google Scholar
  5. 5.
    Wang G, Liu CT, Wang ZL, et al. Antisense oligonucleotides-induced local blockade of T-bet expression leads to airway inflammation in rats. Acta Pharmacol Sin 2006;27(5): 561–567.PubMedCrossRefGoogle Scholar
  6. 6.
    Finotto S, Neurath MF, Glickman JN, et al. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet. Science 2002;295(5553): 336–338PubMedCrossRefGoogle Scholar
  7. 7.
    Global Initiative for Asthma (GINA). Global strategy for asthma management and prevention: NHLBI/WHO Workshop report. Bethesda: National Heart, Lung and Blood Institute. National Institute of Health, 2002.Google Scholar
  8. 8.
    Shen ZY, Shi SZ, Zha LG, et al. Kidney reinforcement regimen in the treatment of bronchial asthma: endocrinological and immunological studies. J Tradit Chin Med 1982;2(2): 135–140.PubMedGoogle Scholar
  9. 9.
    Li XM, Zhang TF, Sampson H, et al. The potential use of Chinese herbal medicines in treating allergic asthma. Ann Allergy Asthma Immunol 2004;93(Suppl 1): S35–S44.PubMedCrossRefGoogle Scholar
  10. 10.
    Markham AW, Wilkinson JM. Complementary and alternative medicines (CAM) in the management of asthma: an examination of the evidence. J Asthma 2004;41(2): 131–139.PubMedCrossRefGoogle Scholar
  11. 11.
    Yu JL, Dou DQ, Chen XH, et al. Protopanaxatriol-type ginsenosides differentially modulate type 1 and type 2 cytokines production from murine splenocytes. Planta Med 2005;71(3): 202–207.PubMedCrossRefGoogle Scholar
  12. 12.
    Ko E, Park JW, Rho S, et al. Chung-Yeul-Gue-Soup-Sa-Gan-Tang, traditional Korean medicine, enhances CD4(+) T cell activates and modulates Th1/Th2 lineage development. J Pharmacol Sci 2004;94(4): 359–367.PubMedCrossRefGoogle Scholar
  13. 13.
    Yesilada E, Bedir E, Calis I, et al. Effects of triterpene saponins from Astragalus speies on in vitro cytokines release. J Ethnopharmacol 2005;96(1–2): 71–77.PubMedCrossRefGoogle Scholar
  14. 14.
    Wei H, Sun R, Xiao W, et al. Traditional Chinese medicine, Astragalus, reverses predominance of Th2 cytokines and their upstream transcript factors in lung cancer patients. Oncol Rep 2003;10(5): 1507–1512.PubMedGoogle Scholar
  15. 15.
    Mao SP, Cheng KL, Zhou YF. Modulatory effect of Astragalus membranaceus on Th1/Th2 cytokine in patients with herpes simplex keratitis. Chin J Integr Tradit West Med 2004;24(2): 121–123.Google Scholar
  16. 16.
    World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA 2000;284(23): 3043–3045.Google Scholar
  17. 17.
    Romagnani S. The role of lymphocytes in allergic disease. J Allergy Clin Immunol 2000;105(3): 399–408.PubMedCrossRefGoogle Scholar
  18. 18.
    Zhang Y, Apilado R, Coleman J, et al. Interferon gamma stabilizes the T helper cell type 1 phenotype. J Exp Med 2001;194(2): 165–172.PubMedCrossRefGoogle Scholar
  19. 19.
    Hardy KJ, Manger B, Newton M, et al. Molecular events involved in regulating human interferon-gamma gene expression during T cell activation. J Immunol 1987;138(7): 2353–2358.PubMedGoogle Scholar
  20. 20.
    Szabo SJ, Sullivan BM, Peng SL, et al. Molecular mechanisms regulating Th1 immune response. Annu Rev Immunol 2003;21: 713–758.PubMedCrossRefGoogle Scholar
  21. 21.
    Afkarian M, Sedy JR, Yang J, et al. T-bet is a STAT1-induced regulator of IL-12R expression in naive CD4+ T cells. Natl Immunol 2002;3(6): 549–557.CrossRefGoogle Scholar
  22. 22.
    Kang H, Ahn KS, Cho C, et al. Immunomodulatory effect of Astragali Radix extract on murine Th1/Th2 cell lineage development. Biol Pharm Bull 2004;27(12): 1946–1950.PubMedCrossRefGoogle Scholar
  23. 23.
    Loetscher P, Uguccioni M, Bordoli L, et al. CCR5 is characteristic of Th1 lymphocytes. Nature 1998;391(6665): 344–345.PubMedCrossRefGoogle Scholar
  24. 24.
    Sallusto F, Mackay CR, Lanazvecchia A. Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells. Science 1997;277(5334): 2005–2007.PubMedCrossRefGoogle Scholar
  25. 25.
    Ylikoski E, Kinos R, Sirkkanen N, et al. Association stuty of 15 novel single-nucleotide polymorphisms of the T-bet locus among Finnish asthma families. Clin Exp Allergy 2004; 34(7): 1049–1055.PubMedCrossRefGoogle Scholar

Copyright information

© Chinese Association of Traditional and Western Medicine, China Academy of Chinese Medical Sciences 2006

Authors and Affiliations

  • Wang Gang  (王刚)
    • 1
  • Liu Chun-tao  (刘春涛)
    • 2
    Email author
  • Wang Zeng-li  (王曾礼)
    • 2
  • Yan Cun-liang  (颜存粮)
    • 3
  • Luo Feng-ming  (罗凤鸣)
    • 2
  • Wang Lei  (王蕾)
    • 1
  • Li Ting-qian  (李廷谦)
    • 1
  1. 1.Department of Integrated Traditional Chinese and Western Medicine, West China HospitalSichuan UniversityChengduChina
  2. 2.Department of Respiratory Disease, West China HospitalSichuan UniversityChina
  3. 3.Department of Laboratory MedicineShenzhen Hospital Affiliated to Peking UniversityChina

Personalised recommendations