Abstract
Xyloglucan endotransglycosylase is an identifi ed relaxation factor with functions of easing and extending of plant cell walls. Its activities are directly related to plant growth and elongation of organisms. Anthocephalus chinensis is a tall and fast growing evergreen tree species. We cloned the full cDNA sequence of AcEXT genes which is abundantly expressed in the cambium of A. chinensis. The sequence analysis of nucleotides and amino acids revealed the presence of a 1396 bp full cDNA sequence, including a 960 bp complete open reading frame (ORF) encoding a 320 amino acid protein. The deduced amino acid sequence of AcXET was homologous to the other known XET proteins and contained the conserved EIDFE catalytic site which was specifi c to all the XETs. Our data should serve as a foundation for further insight into AcXET gene molecular mechanisms during wood formation and cell wall engineering of woody plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cosgrove D J. 1997. Relation in a high-stress environment: the molecular bases of extensible cell walls and cell enlargement. Plant Cell, 9: 1031–1041
Cosgrove D J. 2000. Loosening of plant cell walls by expansins. Nature, 407: 321–326
Cosgrove D J. 2005. Growth of the plant cell wall. Nat Rev Mol Cell Biol, 6: 850–861
Fry S C. 1998. The Growing Plant Cell Wall: Chemical and Metabolic Analysis. Essex: Longman
Herbers K, Lorences E P, Barrachina C, Sonnewald U. 2001. Functional characterization of Nicotiana tabacum xyloglucan endotransglycosylase (NtXET-1): Generation of transgenic tobacco plants and changes in cell wall xyloglucan. Planta, 212(2): 279–287
Patrik J, Stuart D, Harry B, Johansson P, Denman S, Brumer H, Kallas A M, Henriksson H, Bergfors T, Teeri T T, Jones T A. 2003. Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula×tremuloides. Acta Cryst, 59(3): 535–537
Li N, Zhang D F, Pian R Q, Li W, Chen X Y. 2008. Comparative study on methods of RNA Isolation from phloem of Anthocephalus chinensis. J Anhui Agr Sci, 36(11): 4415–4418 (in Chinese with English abstract)
Li W, Li H, Chen X Y, Wu H. 2007. Single nucleotide polymorphism discovery of Pinus radiata with chromosome walking PCR method. Acta Bot Boreal-Occident Sin, 27(8): 1571–1576 (in Chinese with English abstract)
Li W, Li H, Wu H, Chen X Y. 2010. Use of chromosome walking in discovery of single-nucleotide polymorphism in noncoding regions of a candidate actin gene in Pinus radiate. J Appl Genet, 51(3): 275–281
Lu W, Wang Y, Jiang Y, Li J, Liu H, Duan X, Song L. 2006. Differential expression of litchi XET genes in relation to fruit growth. Plant Physiol Biochem, 44(11–12): 707–713
Johansson P, Brumer H, Baumann M J, Kallas A M, Henriksson H, Denman S E, Teeri T T, Jones T A. 2004. Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding. Plant Cell, 16(4): 874–886
Ma S J, Zhu S L, Li W, Ouyang K X, Li N, Chen X Y. 2010. Cloning of XET gene from Anthocephalus chinensis and its plant expression vector construction. Forest Stud China, 12(2): 79–84
Reidy B, Nosberger J, Fleming A. 2001. Differential expression of XET-related genes in the leaf elongation zone of F. pratensis. J Exp Bot, 52(362): 1847–1856
Romo S, Jimenez T, Labraor E, Dopico B. 2005. The gene for a xyloglucan endotransglucosylase/hydrolase from Cicer arietinum is strongly expressed in elongating tissues. Plant Physiol Biochem, 43(2): 169–176
Su G R, Yi G N. Yang Q. 2007. A study study on the growth growth character character of Anthocephalus chinensis. J Northwest Forest Univ, 22(5): 49–52 (in Chinese with English abstract)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, W., Zhu, Sl., Li, N. et al. Characteristics of nucleotides and amino acids in cDNA sequence of xyloglucan endotransglycosylase cloned from Anthocephalus chinensis . For. Stud. China 13, 45–51 (2011). https://doi.org/10.1007/s11632-011-0101-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11632-011-0101-2