Abstract
The hormone combinations and dark culture of the callus induction for Cyclocarya paliurus (Batal.) Iljinskaja leaves were optimized by an orthogonal experiment. The effects of hormones on callus proliferation were also studied. The results show that the optimum medium for callus induction of C. paliurus leaves was MS + 1.0 mg·L−1 6-BA + 0.5 mg·L−1 NAA + 1.0 mg·L−1 2,4-D. The combination of 6-BA and NAA significantly affected the induction of callus, while the effect of 2,4-D was less. The maximum rate of callus induction was 95.4%. A 10-day darkness period was beneficial for callus induction. The best proliferation medium was 1/4 DKW + 0.5 mg·L−1 6-BA + 1.0 mg·L−1 2,4-D.
This is a preview of subscription content, log in via an institution to check access.
References
Driver J A, Kuniyuki A H. 1984. In vitro propagation of Paradox walnut rootstock (Juglans hindsii × Juglans regia, tissue culture). Plant Prod, 19(4): 507–509
Editorial Board of Flora of China of Chinese Academy of Sciences. 1979. Flora of China: Vol. 21. Beijing: Science Press, 18–19 (in Chinese)
Hu D N, Jiang Y, Wu S F, Yang G Y, Shangguan X C. 2005. A preliminary study on tissue culture of Cyclocarya paliurus (Batal.) Iljinskaja. Acta Agric Univ Jiangxi, 27(1): 39–41 (in Chinese with English abstract)
Huang B B, Xiao F Y, Zhang W P, Fu Y Y, Xu J X, Ye D L. 2004. Effect of Cyclocarya paliurus on immunologic function in mice. J Jiangxi Coll TCM, 16(5): 59–60 (in Chinese)
Li L, Xie M Y, Yi X, Wang X R. 2001. Bioactivity of polysaccharide from Cyclocarya paliurus (Batal.) Iljinsk leaves in reducing blood sugar. Acta Agric Univ Jiangxi, 23(4): 484–486 (in Chinese with English abstract)
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15: 473–497
Shangguan X C, Chen J P, Wu S F, Huang Z W, Tang K J, Shu H S, Xu M S. 2003. Hypoglycemic effects of Cyclocarya paliurus (Batal.) Iljinsk extracts on diabetic rabbit. J Northwest ci-Tech Univ Agric For (Nat Sci Ed), 31(6): 117–120 (in Chinese with English abstract)
Shangguan X C, Guo C L, Yang W Y, Jiang Y, Shen Y G. 2006. Effects of basic media and culture conditions on callus growth and flavonoid content of Cyclocarya paliurus. J Fujian Agric For Univ (Nat Sci Ed), 35(6): 588–592 (in Chinese with English abstract)
Shangguan X C, Guo C L, Jiang Y, Shen Y G, Wu S F, Hu D N. 2006. A study on effects of media and plant hormones on callus induction of Cyclocarya paliurus (Batal.) Iljinskaja stems and leaves in vitro. Acta Agric Univ Jiangxi, 28(5): 679–682 (in Chinese with English abstract)
Wang W J, Jiang Y, Wu S F, Shen Y G, Wu H L, Xu M S, Huang Z W, Shu H G, Shangguan X C. 2003. Hypoglycemic effects of Cyclocarya paliurus (Batal.) Iljinsk ethanol abstracts on diabetic mice. Acta Vet et Zoot Sin, 34(6): 562–565 (in Chinese with English abstract)
Yi X, Xie M Y, Gu Q. 2000. Plot study on regulating effect of Cyclocarya paliurus (Batal.) Iljinsk to cholesterin. China Commercial Industry, 4: 51–53 (in Chinese)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hu, Dn., Shen, Yg., Mi, Lx. et al. Callus culture of Cyclocarya paliurus (Batal.) Iljinskaja leaves. For. Stud. China 12, 147–150 (2010). https://doi.org/10.1007/s11632-010-0307-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11632-010-0307-8