Abstract
To develop a transformation protocol of Rosa hybrida ‘Samantha’ via Agrobacterium tumefaciens, the authors examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing β-glucuronidase gene. The results indicate that explant, light condition, salt concentration and acetosyringone (AS) concentration in co-culture medium are the most important factors, and factors like co-culture temperature, co-culture period and bacteria density have a strong effect on the growth of bacteria and then T-DNA transfer. Optimized co-cultivation was performed by inoculation of embryogenic callus with bacteria at a density of OD600=0.5–0.8 for 20 min and co-culture in darkness under 23°C on medium with 1/2 MS salts and 300 µmol·L−1 AS for 3 d.
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[Supported by the National Natural Science Foundation of China (Grant No.30170666)]
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Gao, L., Bao, M. Optimization of the uidA gene transfer of Rosa hybrida via Agrobacterium tumefaciens: an assessment of factors influencing the efficiency of gene transfer. For. Stud. China 6, 9–14 (2004). https://doi.org/10.1007/s11632-004-0034-0
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DOI: https://doi.org/10.1007/s11632-004-0034-0