Jiuzhaigou, characterized by its magnificent waterscapes and subalpine karstic features, is both a World Heritage Site and a World Biosphere Reserve in southwestern China. In recent years, this unique ecosystem has shown signs of stress due to increasing tourism activities within the reserve. The various routine methods, which monitor physical and chemical properties, do not fully reflect water quality in the subalpine and alpine lakes, while the indicators using aquatic organisms to evaluate the water quality or status of the subalpine lakes are poorly reported. Thus, in this study, benthic diatoms from multiple habitats in Jiuzhaigou were sampled and assessed for water quality monitoring. Canonical Correspondence Analysis (CCA) showed that the canonical coefficients for elevation, water temperature and total nitrogen on the first Canonical Correspondence Analysis axis were -0.84, 0.78 and -0.53, respectively, environmental variables associated with the distribution patterns of benthic diatoms. The dominance of diatom taxa indicative of nutrient enrichment indicates a clear trend toward eutrophication in the Pearl Shoal and Colorful Lake, two of the sites mostly visited by tourists. It was observed that the effect of the type of substratum on diatom community composition is not significant in subalpine lakes. The most dominant species in Jiuzhaigou lakes are the genera Achnanthes, Fragilaria, Cymbella, Cocconeis, Diatoma and Denticula. In combination with dominant and sensitive species in the benthic diatom communities, CCA and CA methods can be used to evaluate the impact of human activities on subalpine karstic lakes. The dominance of diatom taxa is indicative of nutrient enrichment and the results of CCA and CA indicate a clear trend toward eutrophication in the Pearl Shoal and Colorful Lake, two of the sites mostly visited by tourists.
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Anderson FØ, Ring P (1999) Comparison of phosphorus release from littoral and profundal sediments in a shallow, eutrophic lake. Hydrobiologia 408-409: 175–183.
Andrus JM, Winter D, Scanlan M, et al. (2013) Seasonal synchronicity of algal assemblages in three Midwestern agricultural streams having varying concentrations of atrazine, nutrients, and sediment. Science of the Total Environment 458-460: 125–139.
Akkoyunlu A, Akiner ME (2012) Pollution evaluation in streams using water quality indices: A case study from Turkey’s Sapanca Lake Basin. Ecological Indicators 18: 501–511. DOI: 10.1016/j.ecolind.2011.12.018
Bao SK, Tan MC, Zhong ZX (1986) A survey of the algal flora in the Jiuzhaigou Nature Reserve of Sichuan. Journal of Southwest Normal University 3: 56–71.
Bere T, Tundisi JG (2010) The Effects of Substrate Type on Diatom-Based Multivariate Water Quality Assessment in a Tropical River (Monjolinho), São Carlos, SP, Brazil, Water Air Soil Pollution 216: 391–409.
Bere T, Tundisi JG (2012) Applicability of the Pampean Diatom Index (PDI) to streams around Sao Carlos-SP, Brazil. Ecological Indicators 13: 342-246. DOI: 10.1016/j.ecolind.2011.05.003
Blanco S, Álvarez-Blanco I, Cejudo-Figueiras C, et al. (2013) New diatom taxa from high-elevation Andean saline lakes. Diatom Research 28: 13–27.
Delgado C, Pardo I (2015) Comparison of benthic diatoms from Mediterranean and Atlantic Spanish streams: community changes in relation to environmental factors. Aquatic Botany 120(B): 304–314. DOI: 10.1016/j.aquabot.2014.09.010
Descy JP, Coste M (1991) A test of methods for assessing water-quality based on diatoms. International Association of Theoretical and Applied Limnology-Proceedings 24: 2112–2116.
Florida Lakewatch (2004) Trophic State: A Waterbody’s Ability to Support Plants, Fish, and Wildlife. Available: http: //lakewatch.ifas.ufl.edu/circpdffolder/trophic2.pdf. Accessed 20 September 2004.
Gudmundsdottira R, Palssona S, Hannesdottir ER (2013) Diatoms as indicators: The influences of experimental nitrogen enrichment on diatom assemblages in sub-Arctic streams. Ecological Indicators 32: 74–81. DOI: 10.1016/j.ecolind.2013.03.015
Horne AJ, Goldman CR (1994) Limnology Second Edition. New York: McGraw-Hill, Inc.
Hu HJ, Wei YX (2006) Freshwater Algae in China. Beijing: Beijing Science Press. pp 300–416. (In Chinese)
Kargioglu M, Serteser A, Kivrak E, et al. (2012) Relationships between epipelic diatoms, aquatic macrophytes, and water quality in Akarçay Stream, Afyonkarahisar, Turkey. Oceanological and Hydrobiological Studies 41: 74–84.
Kelly MG, Cazaubon A, Coring E (1998) Recommendations for the routine sampling of diatoms for water quality assessments in Europe. Journal of Applied Phycology 10: 215–224.
Kelly MG, Whitton BA (1995) Trophic diatom index-A new index for monitoring eutrophication in rivers. Journal of Applied Phycology 7: 433–444. DOI: 10.1007/BF00003802
Kilroy C, Biggs BJF, Vyverman W, et al. (2006) Benthic diatom communities in subalpine pools in New Zealand: relationships to environmental variables. Hydrobiologia 561: 95–110. DOI 10.1007/s10750-005-1607-1
Kroepfl K, Vladár P, Szabó K, et al. (2006) Chemical and biological characterisation of biofilms formed on different substrata in Tisza river (Hungary). Environmental Pollution 144: 626–631. DOI: 10.1016/j.envpol.2006.01.031
Krammer K, Lange-Bertalot H (1988) Bacillariophyceae. 2. Teil: Bacillariaceae, Epithemiaceae, Surirellaceae. Edited by H. Ettl, J. Gerloff, H. Heynig, and D. Mollenhauer. Süflwasserflora von Mittleuropa, Band 2/2. New York: Gustav Fischer Verlag, Stuttgart.
Kufel L, Biardzka E, Strzalek M (2013) Calcium carbonate incrustation and phosphorus fractions in five charophyte species. Aquatic Botany 109: 54–57. DOI: 10.1016/j.aquabot.2013.04.002
Lavoie I, Campeau S, Grenier M, et al. (2006) A diatom-based index for the biological assessment of eastern Canadian rivers: an application of correspondence analysis (CA). Canadian Journal of Fisheries and Aquatic Sciences 63: 1793–1811. DOI: 10.1139/F06-084
Li SG, Hu XX, Tang Y, et al. (2014) Changes in lacustrine environment due to anthropogenic activities over 240 years in Jiuzhaigou National Nature Reserve, southwest China. Quaternary International 349: 367–375.
Liu SY, Zhang XP, Zeng ZY (2007) Biodiversity of the Jiuzhaigou National Nature Reserve. Chengdu: Sichuan Publishing House of Science and Technology. pp 14–23. (In Chinese)
Luis AT, Alexander AC, Almeida SPF, et al. (2013) Benthic diatom communities in streams from zinc mining areas in continental Canada and Mediterranean climates Portugal. Water Quality Research Journal of Canada 48: 180–191.
Majewska R, Zgrundo A, Lemke P, et al. (2012) Benthic diatoms of the Vistula River estuary (Northern Poland): Seasonality, substrata preferences, and the influence of water chemistry. Phycological Research 60: 1–19. DOI: 10.1111/j.1440-1835.2011.00637.x
McGarigal K, Landguth E, Stafford S (2000) Multivariate Statistics for Wildlife and Ecology Research. New York: Springer.
Michelutti N, Mc Cleary K, Douglas MSV, et al. (2013) Comparison of freshwater diatom assemblages from a high arctic oasis to nearby polar desert sites and their application to environmental inference models. Journal of Physiology 49: 41–53. DOI: 10.1111/jpy.12024
Millie DF, Lowe RL (1983) Studies on Lake Erie’s littoral algae; Host specificity and temporal periodicity of Epiphytic diatoms. Hydrobiologia 99: 7–18.
Moravcová A, Rauch O, Lukavsky J, et al. (2013) The response of epilithic diatom assemblages to sewage pollution in mountain streams of the Czech Republic. Plant Ecology and Evolution 146: 153–166.
Moser KA, Mordecai JS, Reynolds RL, et al. (2010) Diatom changes in two Uinta mountain lakes, Utah, USA: responses to anthropogenic and natural atmospheric inputs. Hydrobiologia 648: 91–108. DOI: 10.1007/s10750-010-0145-7
Ministry of Environmental Protection of the People’s Republic of China (2002) Determination methods for examination of water and wastewater. Beijing: China Environmental Science Press. pp 243–248. (In Chinese)
Potapova M, Charles DF (2005) Choice of substrate in algae-based water quality assessment. Journal of North American Bethological Society 24: 415–427.
Potapova M, Charles DF (2007) Diatom metrics for monitoring eutrophication in rivers of the United States. Ecological Indicators 7: 48–70. DOI: 10.1016/j.ecolind.2005.10.001
Pan YD, Stevenson, RJ; Hill, BH, et al. (1996) Using diatoms as indicators of ecological conditions in lotic systems: A regional assessment. Journal of the North American Benthological Society 15: 481–495. DOI: 10.2307/1467800
Pappas JL (2010) Phytoplankton assemblages, environmental influences and trophic status using canonical correspondence analysis, fuzzy relations, and linguistic translation. Ecological Informatics 5: 79–88.
Rimet F (2012) Recent views on river pollution and diatoms. Hydrobiologia 683: 1–24. DOI: 10.1007/s10750-011-0949-0
Rott E, Cantonati M, Füreder L, et al. (2006) Benthic algae in high elevation streams of the Alps–a neglected component of the aquatic biota. Hydrobiologia 562: 195–216.
Smucker NJ, Vis ML (2013) Can pollution severity affect diatom succession in streams and could it matter for stream assessments. Journal of Freshwater Ecology 28: 329–338. DOI: 10.1080/02705060.2013.764356
Stevenson RJ, Pan YD, Manoylov KM, et al. (2008) Development of diatom indicators of ecological conditions for streams of the western US. Journal of the North American Benthological Society 27: 1000–1016. DOI: 10.1899/08-040.1
Shepard WD, Blinn DW, Hoffman RJ, et al. (2000) Algae of Devils Hole, Nevada, Death Valley National Park. Western North American Naturalist 60: 410–419.
Shubert LE (1984) Algae as ecological indicators. London: Academic press.
Sweat LH, Johnson KB (2013) The effects of fine-scale substratum roughness on diatom community structure in estuarine biofilms. Biofouling 29: 879–890.
van Dam H, Mertens A, Sinkeldam J (1994) A coded checklist and ecological indicator values of freshwater diatoms from the Netherland. Netherlands Journal of Aquatic Ecology 28: 117–133.
Wang CH, Zhang JT (2003) Correlation analysis of relationship between benthic diatom species from Fenhe River. Chongqing Environmental Science 25: 106–107.
Wang LC, Behling H, Lee TQ, et al. (2013) Increased precipitation during the Little Ice Age in northern Taiwan inferred from diatoms and geochemistry in a sediment core from a subalpine lake. Journal of Paleolimnology 49: 619–631. DOI: 10.1007/s10933-013-9679-9
Wang LF, Yang LY, Kong LH, et al. (2014) Spatial distribution, source identification and pollution assessment of metal content in the surface sediments of Nansi Lake, China. Journal of Geochemical Exploration 140: 87–95. DOI: 10.1016/j.gexplo.2014.02.008
Weilhoefer CL, Pan YD (2008) Using change-point analysis and weighted averaging approaches to explore the relationships between common benthic diatoms and in-stream environmental variables in mid-atlantic highlands streams, USA. Hydrobiologia 614: 259–274. DOI: 10.1007/s10750-008-9511-0
Winter JG, Duthie HC (2000) Epilithic diatoms as indicators of stream total N and P concentration. Journal of the North American Benthological Society 19: 32–49.
You QM (2006) Preliminary Studies on Flora of Diatoms from Xinjiang in China. PhD thesis, Shanghai Normal University, Shanghai, China.
Zhang JT (2011) Quantitative Ecology. Beijing: Sciences Press. pp 160–165. (In Chinese)
Zhou X, Gao XF, Yang XD, et al. (2009) Autumn diatom variation in lakes along elevation gradient in the Jiuzhaigou National Park, Sichuan, China. China Journal of Applied and Environmental Biology 15: 161–168.
Zhu CK (2007) Study on the correlation of lake water environment and algae in the core area of Jiuzhaigou. PhD thesis, Southwest University, Chongqin, China.
Zhu HZ, Chen JY (2000) Bacillariophyta of the Xizang Plateau. Beijing: Sciences Press. pp 87–341. (In Chinese)
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Ouyang, Ll., Pan, Yd., Huang, Cm. et al. Water quality assessment of benthic diatom communities for water quality in the subalpine karstic lakes of Jiuzhaigou, a world heritage site in China. J. Mt. Sci. 13, 1632–1644 (2016). https://doi.org/10.1007/s11629-014-3392-7
- Subalpine karstic lakes
- Water quality
- Benthic diatoms
- Community composition
- Canonical correspondence analysis