Summary
Leafy shoots of cauliflower were produced from hypocotyl segments, curd branches (debarassed of existing meristems) and intact curd pieces submitted to various basal media. Adventitious shoot production and axillary bud break exhibited very different nutritive requirements. In each case, an interaction between the plant growth regulators and the composition of the basal medium was discovered. Propagation from curd pieces was dependent on the interaction between plant growth regulators and high sucrose-to-macronutrients ratio in the medium. A medium containing 168 mM sucrose, supplemented with 2.5 µM N6-benzyladenine and 5 µM naphthaleneacetic acid allowed the production of numerous shoots without any hyperhydric symptoms.
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References
Brown, D. C. W.; Leung, D. W. M.; Thorpe, T. A. Osmotic requirement for shoot formation in tobacco callus. Physiol. Plant. 46:36–41; 1979.
Debergh, P. C. Effects of agar brand and concentration on the tissue culture medium. Physiol. Plant. 59:270–276; 1983.
Galiba, G.; Erdei, L. Dependence of wheat callus growth, differentiation and mineral content on carbohydrate supply. Plant Sci. 45:65–70; 1986.
Gaspar, T.; Kevers, C.; Debergh, P.; Maene, L.; Paques, M.; Boxus, P. Vitrification: morphological, physiological and ecological aspects. In: Bonga, J. M.; Durzan, D. J., ed. Cell and tissue culture in forestry. Vol. 1. General principles and biotechnology. Dordrecht: Martinus Nijhoff Publ.; 1987:152–166.
Grout, B. W. W. Cauliflower (Brassica oleracea var. botrytis L.). In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry. Vol. 6. Berlin, Heidelberg: Springer-Verlag; 1988:211–225.
Grout, B. W. W.; Aston, M. J. Transplanting of cauliflower plants regenerated from meristem culture. I. Water loss and water transfer related to changes in leaf wax to xylem regeneration. Hort. Res. 17:1–7; 1977.
Grout, B. W. W.; Crisp, P. Practical aspects of the propagation of cauliflower by meristem culture. Acta Hortic. 78:289–295; 1977.
Grout, B. W. W.; Donkin, M. E. Photosynthesis activity of cauliflower meristem cultures in vitro and at transplanting into soil. Acta Hortic. (Wageningen) 212:323–327; 1987.
Hammersely-Straw, D. R. H.; Thorpe, T. A. Use of osmotic inhibition in studies of shoot formation in tobacco callus cultures. Bot. Gaz. 149:303–310; 1988.
Leshem, B.; Werker, E.; Shalev, D. P. The effect of cytokinins on vitrification in melon and carnation. Ann. Bot. 62:271–276; 1988.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Vandemoortele, J. L.; Billard, J. P.; Boucaud, J.; Gaspar, T. Une méthode efficace de micropropagation du chou-fleur (Brassica oleracea L. var. botrytis). Med. Fac. Landbouww. Rijksuniv. Gent 56/3a:735–741; 1991.
Wardle, K.; Quinlan, A.; Simpkins, I. Abscisic acid and the regulation of water loss in plantlets of Brassica oleracea L. var. botrytis regenerated through apical meristem culture. Ann. Bot. 43:745–752; 1979.
Wardle, K.; Dobbs, E. B.; Short, K. C. In vitro acclimatization of aseptically cultured plantlets to humidity. J. Am. Soc. Hortic. Sci. 108:386–389; 1983.
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Vandemoortele, J.L., Billard, J.P., Boucaud, J. et al. Evidence for an interaction between basal medium and plant growth regulatiors during adventitious or axillary shoot formation of cauliflower. In Vitro Cell.Dev.Biol.-Plant 35, 13–17 (1999). https://doi.org/10.1007/s11627-999-0003-3
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DOI: https://doi.org/10.1007/s11627-999-0003-3