Summary
An in vitro culture system for direct shoot regeneration from cotyledon explants of rapid-cycling Brassica rapa was developed. Cotyledons from 3-d-old seedlings, when cultured on Murashige and Skoog (MS) medium supplemented with 20 µM N6-benzyladenine (BA) and 2 µM α-naphthaleneacetic acid (NAA), regenerated shoots directly at a frequency of 20%. The addition of 2 µM aminoethoxyvinylglycine (AVG) to this medium increased shoot regeneration to 33%, but silver nitrate drastically inhibited shoot regeneration. Shoot regeneration occurred directly, at the petiolar cut ends of cotyledonary explants, between 10 to 17 d in culture. The highest percentage of regeneration (33%) was obtained from 3-d-old seedlings. NAA was the most effective auxin for root induction and development, with 49% of shoots producing roots after 2 wk on medium containing 1.0 µM NAA. Regenerated plantlets were grown to maturity in pots containing peat moss and vermiculite (1:1). These plants were morphologically normal and fertile. With this protocol, over 100 independently derived, flowering R0 plants were obtained from 40 regenerating cotyledonary explants within 40 d after culture initiation.
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Teo, W., Lakshmanan, P., Kumar, P. et al. Direct shoot formation and plant regeneration from cotyledon explants of rapid-cycling Brassica rapa . In Vitro Cell.Dev.Biol.-Plant 33, 288–292 (1997). https://doi.org/10.1007/s11627-997-0052-4
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DOI: https://doi.org/10.1007/s11627-997-0052-4