Abstract
This study was carried out to determine the effects of 24-epibrassinolide (24-eBL) and methyl jasmonate (MeJA) on cell growth and accumulation of secondary metabolites in immobilized cells of Hyoscyamus niger. For this purpose, different combinations of 24-eBL (0.5, 1.0, and 2.0 mg l−1) and MeJA (1.0 mM) were applied to cells immobilized with calcium alginate. At the end of the 30-day applications, fresh and dry cell weights, cell growth index, tropane alkaloids (hyoscyamine and scopolamine), phenolic (gallic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, vanillin, p-coumaric acid, o-coumaric acid, ferulic acid, rutin, cinnamic acid, rosmarinic acid, and quercetin), and total phenolic content were determined. According to the results, 2.0 mg l−1 24-eBL for cell growth, 0.5 mg l−1 24-eBL for scopolamine and hyoscyamine, and all 24-eBL combinations with 1.0 mM MeJA for phenolic compounds were selected as the most effective applications. In conclusion, it was determined that the application of 24-eBL and MeJA may be successfully used to increase the production of secondary metabolites in immobilized cell cultures of H. niger when used in appropriate concentrations and combinations.
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Acknowledgements
The authors thank Isparta University of Applied Sciences Department of Field Crops for providing Hyoscyamus niger seeds.
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SO: formal analysis, investigation. TD: conceptualization, methodology, formal analysis, investigation, writing—original draft, writing—review and editing, supervision, funding acquisition. NGB: writing—review and editing, supervision.
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Özmen, S., Demirci, T. & Baydar, N.G. 24-Epibrassinolide and methyl jasmonate can encourage cell growth and the production of secondary metabolites in immobilized cells of Hyoscyamus niger. In Vitro Cell.Dev.Biol.-Plant 58, 826–836 (2022). https://doi.org/10.1007/s11627-022-10313-5
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DOI: https://doi.org/10.1007/s11627-022-10313-5