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Juglone promotes shooting and inhibits rooting in leaf explants of in vitro raised tomato (Solanum lycopersicum L. var. Pusa Ruby) seedlings

  • Plant Tissue Culture
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Abstract

Juglone is an inhibitor of choline acetyltransferase, an enzyme that catalyzes the synthesis of acetylcholine. Previous works have shown the effect of acetylcholine on in vitro morphogenesis in tomato explants. This work on juglone is designed to indirectly test the presence of acetylcholine biosynthesis in tomato plants. Leaf explants harvested from 30-d-old tomato (Solanum lycopersicum L. variety Pusa Ruby) seedlings were cultured on juglone-fortified control media. Juglone concentrations ranging from 10−9 to 10−3 M were used to check the dose response. It was observed that juglone significantly inhibited rooting and caused transition from rhizogenesis to caulogenesis at 10−7 to 10−6 M. Supplementation of different levels of juglone to the shoot regeneration medium enhanced the shoot formation and callus amounts, exhibiting best response at 10−5 M. This study suggests that juglone regulates morphogenesis in tomato via the acetylcholine system in plants.

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Data availability

All data generated or analyzed during this study are included in this manuscript, and the raw data will be made available from the corresponding author if required.

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Funding

Financial assistance was provided by the University Grants Commission, New Delhi, to RG as a research project entitled “Acetylcholine in Plant Growth and Development” and a Junior Research Fellowship to KB by the Council of Scientific and Industrial Research, New Delhi, India.

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Authors and Affiliations

  1. Department of Botany, Shivaji College, University of Delhi, Delhi, 110027, India

    Kiran Bamel

  2. Department of Botany, University of Delhi, Delhi, 110007, India

    Rajendra Gupta

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  1. Kiran Bamel
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  2. Rajendra Gupta
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Contributions

RG contributed to the study conception and design. The material preparation, data collection, and analysis were performed by KB. The first draft of the manuscript was written by KB and RG read and commented on the previous versions of manuscript. Both authors read and approve the final manuscript.

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Correspondence to Kiran Bamel.

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Bamel, K., Gupta, R. Juglone promotes shooting and inhibits rooting in leaf explants of in vitro raised tomato (Solanum lycopersicum L. var. Pusa Ruby) seedlings. In Vitro Cell.Dev.Biol.-Plant 58, 942–949 (2022). https://doi.org/10.1007/s11627-022-10277-6

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