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An alternative method for medium-term in vitro conservation of different plant species through gibberellin inhibitors

  • Plant Tissue Culture
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Abstract

Medium-term in vitro plant conservation is an alternative for maintaining germplasm banks in breeding programs. The aim of this study was to evaluate the efficiency of the gibberellin inhibitors paclobutrazol (PBZ) and trinexapac-ethyl (TNE) for in vitro conservation by minimal growth in anthurium, sugarcane, and agave. For the establishment of the conservation system, shoots of the aforementioned species were taken at different concentrations (0, 1, 2, and 3 mg L−1) of PBZ and TNE. At 180 d of culture, the survival percentage, number of shoots, plant length, number of leaves, number of roots, root length, total chlorophyll content, stomatal density, and survival percentage during acclimatization were evaluated. According to the variables evaluated, the results showed that in anthurium 1 mg L−1 PBZ was the best treatment for in vitro conservation without affecting survival, while in sugarcane it was 1 mg L−1 PBZ and agave 3 mg L−1 PBZ or TNE. Survival during acclimatization was 97, 96, and 98% in anthurium, sugarcane, and agave, respectively. In this study, the gibberellin-inhibiting compounds PBZ and TNE are a low-cost alternative for in vitro conservation of anthurium, sugarcane, and agave and can be evaluated in other species.

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Data availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Colegio de Postgraduados Campus Córdoba, Km. 348 Carretera Federal Córdoba-Veracruz, 94953, Amatlán de los Reyes, Veracruz, Mexico

    José Luis Spinoso-Castillo, Juan Antonio Pérez-Sato & Sandra Silvana Schettino-Salomón

  2. CONACYT-Colegio de Postgraduados Campus Córdoba, Km. 348 Carretera Federal Córdoba-Veracruz, 94953, Amatlán de los Reyes, Veracruz, Mexico

    Jericó Jabín Bello-Bello

Authors
  1. José Luis Spinoso-Castillo
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  2. Juan Antonio Pérez-Sato
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  3. Sandra Silvana Schettino-Salomón
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  4. Jericó Jabín Bello-Bello
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Contributions

José Luis Spinoso-Castillo designed and carried out the experiments, analyzed the data, and wrote the manuscript. Sandra Silvana Schettino-Salomón carried out the experiments and helped in writing the manuscript. Juan Antonio Pérez-Sato helped in preparing the manuscript. Jericó Jabín Bello-Bello is the lab head. All authors approved the final version of the manuscript for publication.

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Correspondence to Jericó Jabín Bello-Bello.

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Spinoso-Castillo, J.L., Pérez-Sato, J.A., Schettino-Salomón, S.S. et al. An alternative method for medium-term in vitro conservation of different plant species through gibberellin inhibitors. In Vitro Cell.Dev.Biol.-Plant 58, 606–614 (2022). https://doi.org/10.1007/s11627-022-10263-y

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  • DOI: https://doi.org/10.1007/s11627-022-10263-y

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