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Thidiazuron-induced efficient biosynthesis of phenolic compounds in callus culture of Ipomoea turbinata Lagasca and Segura

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Abstract

Ipomoea turbinata Lagasca and Segura (Purple Moonflower) belongs to the largest flowering genus Ipomoea in the Convolvulaceae family. Ipomoea turbinata has not been previously explored for its in vitro potential. This is the first study focused on thidiazuron-induced callus culture for efficient biosynthesis of commercially important phenolic compounds in this plant species. Among the two plant growth regulators tested on leaf, stem, and root explants, 5 mg L−1 thidiazuron (TDZ) induced the highest biomass accumulation (61.4 g L−1 fresh weight, 6.3 g L−1 dry weight) in leaf-derived callus cultures after 5 wk of culture. The highest total phenolic and flavonoid contents recorded were 9.04 mg g−1 and 1.16 mg g−1, respectively, in optimized callus cultures. High-performance liquid chromatography analysis indicated high levels of pharmacologically important anticancer compounds such as chlorogenic acid (13.48 mg g−1), arctigenin (11.67 mg g−1), quercetin (6.19 mg g−1), and kaempferol (5.48 mg g−1), along with other phenolic acids. Furthermore, the antioxidant activity was also evaluated, and leaf-derived callus culture displayed a maximum of 62.6% antioxidant potential. The induction of improved biomass accumulation in callus culture and the production of multipotent bioactive metabolites shows the potential of the multifunctional thidiazuron hormone as an efficient elicitation tool in callus culture of I. turbinata.

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Acknowledgments

Dr. Bilal Haider Abbasi acknowledges the research fellowship of Le Studium-Institute for Advanced Studies, Loire Valley, Orléans, France. This research was in part supported by Cosmetosciences, a global training and research program dedicated to the cosmetic industry. The authors appreciate language editing of manuscript by Muhammad Younas.

Funding

Located in the heart of the cosmetic valley, this program led by University of Orléans, France, is funded by the Region Centre-Val de Loire.

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Authors and Affiliations

  1. Department of Biotechnology, Quaid-I-Azam University, Islamabad, 45320, Pakistan

    Waqar Ahmad, Adnan Zahir, Muhammad Nadeem, Muhammad Zia & Bilal Haider Abbasi

  2. Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Plant Lignans Team, INRA USC1328, Université d’Orléans, Chartres, France

    Christophe Hano & Bilal Haider Abbasi

  3. EA2106 Biomolecules et Biotechnologies Vegetales, Universite Francois-Rabelais de Tours, Tours, France

    Bilal Haider Abbasi

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  1. Waqar Ahmad
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  2. Adnan Zahir
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  3. Muhammad Nadeem
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  4. Muhammad Zia
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  5. Christophe Hano
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  6. Bilal Haider Abbasi
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Contributions

WA performed experiments of establishment of callus culture, optimization with TDZ, and analyses of TPC, TFC, and antioxidant activity. AZ and MN assisted WA with all of these experiments. BHA conceived idea, supervised research, and critically evaluated MS. CH contributed to HPLC analysis and critical revision of manuscript. MZ assisted with experiments and manuscript preparation.

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Correspondence to Bilal Haider Abbasi.

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Ahmad, W., Zahir, A., Nadeem, M. et al. Thidiazuron-induced efficient biosynthesis of phenolic compounds in callus culture of Ipomoea turbinata Lagasca and Segura. In Vitro Cell.Dev.Biol.-Plant 55, 710–719 (2019). https://doi.org/10.1007/s11627-019-10027-1

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  • DOI: https://doi.org/10.1007/s11627-019-10027-1

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