Skip to main content

A new temporary immersion system for commercial micropropagation of banana (Musa AAA cv. Grand Naine)

In Vitro Cellular & Developmental Biology - Plant volume 55, pages 313–320 (2019)Cite this article

Abstract

Temporary immersion systems (TIS) are among the best options for commercial micropropagation. However, although successes have been reported with TIS, it is necessary to establish the most suitable system. The aim of this research was to compare the efficiency of different banana (Musa AAA cv. Grand Naine) micropropagation systems. In addition, a liquid medium with partial immersion and a semi-solid culture medium were evaluated. At 28 d of culture, the number and length of shoots, number of leaves per shoot, chlorophyll content, stomatal index, and the percentage of closed stomata were recorded. Additionally, the survival percentage and ex vitro development of the plantlets were evaluated during acclimatization. Results showed that TIS have a higher multiplication rate with respect to partial immersion and semi-solid medium. The chlorophyll content, stomatal index, and stomata functioning were affected by the different culture systems. The different culture systems had no effect on the survival percentage, although morphological differences were observed during acclimatization. The SETIS™ bioreactor was more efficient and resulted in better development in the plants obtained in vitro and ex vitro, which proved this system to be a useful alternative for banana micropropagation.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

References

  • Alvard D, Cote F, Teisson C (1993) Comparison of methods of liquid medium culture for banana micropropagation: effects of temporary immersion of explants. Plant Cell Tissue Organ Cult 32:55–60

    Article  Google Scholar 

  • Aragón CE, Escalona M, Capote I, Pina D, Cejas I, Rodríguez R, Cañal MJ, Sandoval J, Roels S, Deberch P, Gonzalez-Olmedo J (2005) Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization. In Vitro Cell Dev Biol-Plant 41:550–554

    Article  CAS  Google Scholar 

  • Aragón CE, Escalona M, Rodriguez R, Cañal MJ, Capote I, Pina D, González-Olmedo J (2010) Effect of sucrose, light, and carbon dioxide on plantain micropropagation in temporary immersion bioreactors. In Vitro Cell Dev Biol-Plant 46:89–94

    Article  CAS  Google Scholar 

  • Aragón CE, Sánchez C, Gonzalez-Olmedo J, Escalona M, Carvalho L, Amâncio S (2014) Comparison of plantain plantlets propagated in temporary immersion bioreactors y gelled medium during in vitro growth y acclimatization. Biol Plant 58:29–38

    Article  CAS  Google Scholar 

  • Asayesh MZ, Vahdati K, Aliniaeifard S, Askari N (2017) Enhancement of ex vitro acclimation of walnut plantlets through modification of stomatal characteristics in vitro. Sci Hortic 220:114–121

    Article  Google Scholar 

  • Büssis D, Von Groll U, Fisahn J, Altman T (2006) Stomatal aperture can compensate altered stomatal density in Arabidopsis thaliana at growth light conditions. Funct Plant Biol 33:1037–1043

    Article  Google Scholar 

  • Dale J, James A, Paul JY, Khanna H, Smith M, Peraza-Echeverria S, Garcia-Bastidas F, Kema G, Waterhouse P, Mengersen K, Harding R (2017) Transgenic Cavendish bananas with resistance to Fusarium wilt tropical race 4. Nat Commun 8:1496

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ducos JP, Labbe G, Lambot C, Pétiard V (2007) Pilot scale process for the production of pre-germinated somatic embryos of selected robusta (Coffea canephora) clones. In Vitro Cell Dev Biol-Plant 43:652–659

    Article  Google Scholar 

  • Escalona M, Lorenzo JC, Gonzalez B, Danquita M, Desjardins Y, Barroto CG (1999) Pinneaple (Ananas Comosus L. Merr), micropropagation in temporaty immersion system. Plant Cell Rep 18:743–178

    Article  CAS  Google Scholar 

  • Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tissue Organ Cult 69:215–231

    Article  Google Scholar 

  • Etienne H, Bertrand B, Georget F, Lartaud M, Montes F, Dechamp E, Verdeil JL, Barry-Etienne D (2013) Development of coffee somatic and zygotic embryos to plants differs in the morphological, histochemical and hydration aspects. Tree Physiol 33:640–653

    Article  CAS  PubMed  Google Scholar 

  • Fu X, Cheng S, Liao Y, Huang B, Du B, Zeng W, Jiang Y, Duan X, Yang Z (2018) Comparative analysis of pigments in red and yellow banana fruit. Food Chem 239:1009–1018

    Article  CAS  Google Scholar 

  • Georgiev V, Schumann A, Pavlov A, Bley T (2014) Temporary immersion systems in plant biotechnology. Eng. Life Sci 14:607–621

    Article  CAS  Google Scholar 

  • Harborne JB (1973) Nitrogen compounds. In: Harborne JB (ed) Phytochemical methods. Springer, Dordrecht, pp 166–211

    Chapter  Google Scholar 

  • Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Horti 108(2):105–120

    Article  CAS  Google Scholar 

  • Monja-Mio KM, Barredo PF, Herrera HG, Esqueda VM, Robert ML (2015) Development of the stomatal complex and leaf surface of Agave angustifolia Haw. ‘Bacanora’ plantlets during the in vitro to ex vitro transition process. Sci Hortic 189:32–40

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth y bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Ramírez-Mosqueda MA, Iglesias-Andreu LG (2016) Evaluation of different temporary immersion systems (BIT®, BIG, and RITA®) in the micropropagation of Vanilla planifolia Jacks. In Vitro Cell Dev Biol-Plant 52:154–160

    Article  Google Scholar 

  • Roels S, Noceda C, Escalona M, Sandoval J, Canal MJ, Rodríguez R, Debergh P (2006) The effect of headspace renewal in a temporary immersion bioreactor on plantain (Musa AAB) shoot proliferation and quality. Plant Cell Tissue Organ Cult 84:155–163

    Article  Google Scholar 

  • Steingroewer J, Bley T, Georgiev V, Ivanov I, Lenk F, Marchev A, Pavlov A (2013) Bioprocessing of differentiated plant in vitro systems. Eng Life Sci 13:26–38

    Article  CAS  Google Scholar 

  • Tisserat B, Vandercook CE (1985) Development of an automated plant culture system. Plant Cell Tissue Organ Cult 5:107–117

    Article  Google Scholar 

  • Vervit (2018) SETIS™ Bioreactor Temporary immersion systems in plant micropropagation. http://www.setis-systems.be. Cited 10 de Feb 2018

  • Vieira LN, Fraga HPF, dos Anjos KG, Puttkammer CC, Scherer RF, da Silva DA, Guerra MP (2015) Light-emitting diodes (LED) increase the stomata formation and chlorophyll content in Musa acuminate (AAA) ‘Nanicão Corupá’ in vitro plantlets. Theor Exp Plant Physiol 27:91–98

    Article  CAS  Google Scholar 

  • Wang F, Chen ZH, Shabala S (2017) Hypoxia sensing in plants: on a quest for ion channels as putative oxygen sensors. Plant Cell Physiol 58:1126–1142

    Article  CAS  PubMed  Google Scholar 

  • Wilken D, Gonzalez EJ, Gerth A, Gómez-Kosky R, Schumann A, Claus D (2014) Effect of immersion systems, lighting, and TIS designs on biomass increase in micropropagating banana (Musa spp. cv. ‘Grande naine’AAA). In Vitro Cell Dev Biol-Plant 50:582–589

    Article  CAS  Google Scholar 

  • Wilkinson H (1979) The plant surface (mainly leaf). In: Metcalfe CR, Chalk L (eds) Anatomy of dicotyledons. Claredon Press, Oxford, pp 97–165

    Google Scholar 

  • Wu HC, Kuo ML, Chen CM (2018) Promotion of vegetative growth in force-ventilated Protea cynaroides L. explants cultured in modified temporary immersion culture vessels. HortScience 53:231–235

    Article  CAS  Google Scholar 

  • Xu Z, Zhou G (2008) Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass. J Exp Bot 59:3317–3325

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

The CONACYT-Colegio de Postgraduados-Campus Córdoba financially supported the study.

Author information

Authors and Affiliations

  1. CONACYT-Colegio de Postgraduados-Campus Córdoba, Carretera federal Córdoba-Veracruz Km. 348, 94946, Amatlán de los Reyes, Veracruz, Mexico

    Jericó Jabín Bello-Bello

  2. Universidad Veracruzana-Facultad de Ciencias Químicas, Oriente 6, No. 1009, 94340, Orizaba, Veracruz, Mexico

    Carlos Alberto Cruz-Cruz

  3. VERVIT bvba, Karnemelkstraat 2-4, 9060, Zelzate, Belgium

    Juan Carlos Pérez-Guerra

Authors
  1. Jericó Jabín Bello-Bello
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Carlos Alberto Cruz-Cruz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Juan Carlos Pérez-Guerra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jericó Jabín Bello-Bello.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bello-Bello, J.J., Cruz-Cruz, C.A. & Pérez-Guerra, J.C. A new temporary immersion system for commercial micropropagation of banana (Musa AAA cv. Grand Naine). In Vitro Cell.Dev.Biol.-Plant 55, 313–320 (2019). https://doi.org/10.1007/s11627-019-09973-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-019-09973-7

Keywords

  • Temporary immersion systems
  • Chlorophyll
  • Stomata
  • Acclimatization