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Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality

Abstract

Sweet cherry (Prunus avium L.) varieties and cherry rootstocks are an important part of the fruit industry, and difficulties associated with mass propagation provide an opportunity for the use of temporary immersion systems (TIS). We show the establishment of culture procedures for four genotypes: the rootstocks Maxma-14 and Colt and the varieties ‘Van’ and ‘Rainier.’ The starting explants were internodal segments from seedlings kept in solid propagation medium (PM) (Driver-Kuniyuki Walnut (DKW) base supplemented with indole butyric acid, benzyl amino purine; ascorbic acid, myo-inositol, and agar). Segments were cultured under TIS for 14 d and led to whole plant generation after 30 d of culturing in solid rooting media, which depended on whether they are varieties or rootstocks. A 15-d acclimatization phase led to establishment in greenhouse. The efficiency of TIS was specifically analyzed for the two best PM-derivative media and compared to cultures using solid medium. A number of shoots (P x), biomass (Q x), and sucrose consumption (SC) were evaluated for these purposes. The results showed that Maxma-14, Colt, and ‘Van’ TIS cultures had improved performance in comparison to solid cultures, whereas ‘Rainier’ showed no differences. The number of immersions influenced all of the productive parameters (P x, Q x, and SC), whereas genotype affected P x, and the time of immersion influenced SC. The best Q x and P x values were obtained with the rootstocks Maxma-14 and Colt, as well as the variety Van; these showed no hyperhydration. Physiological studies show that 14-d TIS-produced shoots represented an intermediate stage between solid-derived and adult plants, although the photosynthetic efficiencies of these materials revealed a lack of autotrophic ability at this point.

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References

  • Adelberg J, Fári MG (2010) Applied physiology and practical bioreactors for plant propagation. Propag Ornam Plants 10:205–219

    Google Scholar 

  • Akdemir H, Süzerer V, Onay A, Tilkat E, Ersali Y, Çiftçi YO (2014) Micropropagation of the pistachio and its rootstocks by temporary immersion system. Plant Cell Tissue Organ Cult 117:65–76. https://doi.org/10.1007/s11240-013-0421-0

    CAS  Article  Google Scholar 

  • Alanagh E, Garoosi G, Haddad R, Maleki S, Landí M, Gallego P (2014) Design of tissue culture media for efficient Prunus rootstock micropropagation using artificial intelligence models. Plant Cell Tissue Organ Cult 117:349–359

    CAS  Article  Google Scholar 

  • Amiri M (2006) In vitro techniques to study the shoot-tip grafting of Prunus avium L. (cherry) var. Seeyahe Mashad. J Food Agric Environ 4:151–154

    Google Scholar 

  • Arigita L, Cañal J, Sánchez R, González A (2010) CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson). In Vitro Cell Dev Biol Plant 46:312–322

    CAS  Article  Google Scholar 

  • Baker NR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89–113

    CAS  Article  PubMed  Google Scholar 

  • Bhagwat B, Lane WD (2004) In vitro shoot regeneration from leaves of sweet cherry (Prunus avium) ‘Lapins’ and ‘Sweetheart’. Plant Cell Tissue Organ Cult 78:173–181

    CAS  Article  Google Scholar 

  • Buwalda JG, Noga G (1994) Intra-plant differences in leaf chlorophyll fluorescence parameters in perennial fruiting plants. New Zeal J Crop Hort 22:373–380

    Article  Google Scholar 

  • Canli FA, Tian L (2008) In vitro shoot regeneration from stored mature cotyledons of sweet cherry (Prunus avium L.) cultivars. Sci Hortic 116:34–40

    CAS  Article  Google Scholar 

  • Carrasco B, Meisel L, Gebauer M, Garcia-Gonzales R, Silva H (2013) Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective. Biol Res 46:219–230

    Article  PubMed  Google Scholar 

  • Chakrabarty D, Hahn EJ, Yoon YS, Paek KY (2003) Micropropagation of apple root stock ‘M9 EMLA’ using bioreactor. J Hortic Sci Biotechnol 78:605–609

    CAS  Article  Google Scholar 

  • Chakrabarty D, Park SY, Ali MB, Shin KS, Paek KY (2006) Hyperhydricity in apple: physiological and ultrastructural aspects. Tree Physiol 26:377–388

    CAS  Article  PubMed  Google Scholar 

  • Damiano C, Caboni E, Frattarelli A, Giorgioni M, Liberali M, Lauri P, D'Angeli S (2000) Innovative micropropagation of temperate fruit trees: the case of pear. Acta Hort 530:181–185

    Article  Google Scholar 

  • Damiano C, La Starza SR, Monticelli S, Gentile A, Caboni E, Frattarelli A (2005) Propagation of Prunus and Malus by temporary immersion. In: Hvoslef-Eide AK, Preil W (eds) Liquid culture systems for in vitro plant propagation. Springer, Dordrecht, pp 243–251

  • Driver J, Kuniyuki A (1984) In vitro propagation of paradox walnut rootstock. Hortscience 19:507–509

    Google Scholar 

  • Druart P (2013) Micropropagation of Prunus species relevant to cherry fruit production. In: Lambardi M, Ozudogru EA, Jain SM (eds) Protocols for micropropagation of selected economically-important horticultural plants. Springer Science+Business Media, New York, pp 119–136

    Google Scholar 

  • Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tissue Organ Cult 69:215–231

    Article  Google Scholar 

  • Feeney M, Bhagwat B, Mitchell J, Lane D (2007) Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.) Plant Cell Tissue Organ Cult 90:201–214

    Article  Google Scholar 

  • Feucht W, Dausent B (1976) Root induction in vitro of easy-to-root Prunus pseudocerasus and difficult-to-root Prunus avium. Scientia Hortic 4:439–454

    Article  Google Scholar 

  • Hartman HT, Brooks RM (1976) Propagation of Stockton Morello cherry rootstock by softwood cuttings under mist sprays. Proc Amer Soc Hort Sei 71:127–134

    Google Scholar 

  • Hammatt N, Grant NJ (1998) Shoot regeneration from leaves of Prunus serotina Ehrh. (black cherry) and P. avium L. (wild cherry). Plant Cell Rep 17:526–530

    CAS  Article  Google Scholar 

  • Harris RE, Mason EB (1983) Two machines for in vitro propagation of plants in liquid media. Can J Plant Sci 63:311–316

    Article  Google Scholar 

  • Horsley SB, Gottschalk KW (1993) Leaf area and net photosynthesis during development of Prunus serotina seedlings. Tree Physiol 12:55–69

    CAS  Article  PubMed  Google Scholar 

  • Ivaniĉka J, Pret’ová A (1980) Embryo culture and micropropagation of cherries in vitro. Sci Hortic 12:77–82

    Article  Google Scholar 

  • Klughammer C, Schreiber U (2008) Complementary PS II quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the saturation pulse method. PAM Application Notes 1:27–35

    Google Scholar 

  • McAlister B, Finnie J, Watt MP, Blakeway F (2005) Use of temporary immersion system (RITA) for production of commercial Eucalyptus clones in Mondi forests (SA). Plant Cell Tissue Organ Cult 81:347–358

    Article  Google Scholar 

  • Mahdavian M, Bouzari N, Abdollah H (2011) Effects of media and plant growth regulators on micropropagation of a dwarfing cherry rootstock (PHL-A). Bih Biol 5:86–90

    Google Scholar 

  • Marchi S, Tognetti R, Minnocci A, Borghi M, Sebastiani L (2008) Variation in mesophyll anatomy and photosynthetic capacity during leaf development in a deciduous mesophyte fruit tree (Prunus persica) and an evergreen sclerophyllous Mediterranean shrub (Olea europaea). Trees 22:559–571

    CAS  Article  Google Scholar 

  • Martínez-Gómez P, Sánchez-Pérez R, Rubio M, Dicenta F, Gradziel TM, Sozzi GO (2005) Application of recent biotechnologies to Prunus tree crop genetic improvement. Cienc Invest Agraria 32:73–96

    Google Scholar 

  • Muna A-S, Ahmad A-K, Mahmoud K, Abdul-Rahman K (1999) In vitro propagation of a semi-dwarfing cherry rootstock. Plant Cell Tissue Organ Cult 59:203–208

    CAS  Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    CAS  Article  Google Scholar 

  • Németh G (1979) Benzyladenine-stimulated rooting in fruit-tree rootstocks cultured in vitro. Z Pflanzenphysiol 95:S389–S396

    Article  Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404

    Google Scholar 

  • Özzambak E, Schmit H (1991) In vitro and in vivo micrografting of cherry (Prunus avium L.) Gartenbauwissenschaft 56:221–223

    Google Scholar 

  • Park SY, Moon HK, Murthy HN, Kim YW (2011) Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture. Biol Plant 55:559–562

    Article  Google Scholar 

  • Pérez L, Padrón Y, González J, Rodríguez R, Norman O, Barbón R, Hurtado O, Rodríguez R, Daniels D, Gómez-Kosky R (2015) Effects of different culture conditions (photoautotrophic, photomixotrophic) and the auxin indole-butyric acid on the in vitro acclimatization of papaya (Carica papaya L. var. Red Maradol) plants using zeolite as support. Afr J Biotech 14:2622–2635

    Article  Google Scholar 

  • Quoirin M, Lepoivre P (1977) Etude de milieux adaptes aux cultures in vitro de Prunus. Acta Hort 78:437–442

    Article  Google Scholar 

  • Ruzic D, Vujovic T (2008) The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.) J Hortic Sci 35:12–21

    CAS  Google Scholar 

  • Sedlák J, Paprštein F (2008) In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan. Hort Sci (Prague) 35:95–98

    Google Scholar 

  • Sedlák J, Paprštein F (2011) Micropropagation of sweet cherry cultivars. Vědecké Práce Ovocnářské/ Scientific Papers of Pomology 22:151–157

    Google Scholar 

  • Shin K, Park S, Paek K (2013) Sugar metabolism, photosynthesis, and growth of in vitro plantlets of Doritaenopsis under controlled microenvironmental conditions. In Vitro Cell Dev Biol Plant 49:445–454

    CAS  Article  Google Scholar 

  • Silveira M (1998) Preparo de amostras biológicas para microscopia eletrônica de varredura. In: Souza W (ed) Técnicas Básicas de Microscopia Eletrônica aplicada às Ciências Biológicas. Departamento de Editoração Eletrônica da Universidade Estadual do Norte Fluminense, Rio de Janeiro, pp 33–44

    Google Scholar 

  • Wang H, Wang F, Wang G, Majourhat K (2007) The responses of photosynthetic capacity, chlorophyll fluorescence and chlorophyll content of nectarine (Prunus persica var. Nectarina Maxim) to greenhouse and field grown conditions. Sci Hort 112:66–72

    CAS  Article  Google Scholar 

  • Watt P (2012) The status of temporary immersion system (TIS) technology for plant micropropagation. Afr J Biotechnol 11:14025–14035

    CAS  Google Scholar 

  • Welander M, Persson J, Asp H, Zhu LH (2014) Evaluation of a new vessel system based on temporary immersion system for micropropagation. Sci Hort 79:227–232

    Article  Google Scholar 

  • Xiao Y, Niu G, Kozai T (2011) Development and application of photoautotrophic micropropagation plant system. Plant Cell Tissue Organ Cult 105:149–158

    CAS  Article  Google Scholar 

  • Zilkah S, Faingersh E, Rotbaum A (1992) ln vitro propagation of three M x M cherry rootstocks. Acta Hort 314:93–97

    Article  Google Scholar 

  • Zhu LH, Li XY, Welander M (2005) Optimisation of growing conditions for the apple rootstock M26 grown in RITA containers using temporary immersion principle. Plant Cell Tissue Organ Cult 81:313–318

    Article  Google Scholar 

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Funding

This work was funded by the Biofrutales S.A. Consortium and FONDEF-Chile grant G09i1008.

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Correspondence to Humberto Prieto.

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Editor: Ewen Mullins

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Godoy, S., Tapia, E., Seit, P. et al. Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality. In Vitro Cell.Dev.Biol.-Plant 53, 494–504 (2017). https://doi.org/10.1007/s11627-017-9856-z

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  • DOI: https://doi.org/10.1007/s11627-017-9856-z

Keywords

  • Prunus avium
  • Bioreactor
  • Liquid media
  • Clonal propagation