Abstract
This study evaluated the phytotoxicities of timentin as well as other β-lactam antibiotics on Anthurium andraeanum Linden ex Andre by investigating their effects on callus induction, growth, shoot regeneration, and shoot rooting. At concentrations of 50–500 mg L−1, timentin had null or negligible negative effects on callus induction, proliferation, and shoot regeneration. In contrast, cefoxitin at any concentration other than 50 mg L−1, and ceftazidime, cefonicid, and ceftriaxone at all concentrations tested, prolonged the duration of callus emergence, decreased the percentage of leaves that formed callus, inhibited callus proliferation, and caused chlorosis and death of leaves. Moreover, ceftriaxone and cefonicid at all concentrations tested, and cefoxitin and ceftazidime at concentrations greater than 100 and 200 mg L−1, respectively, reduced the shoot regeneration rate significantly. Timentin, cefonicid, and ceftazidime did not inhibit shoot rooting at either 50 or 100 mg L−1. In contrast, ceftriaxone inhibited shoot rooting at 50 mg L−1. The antibacterial activity of timentin, which had the least toxicity, was tested in planta. After cultivation for 50 d, the growth of Agrobacterium tumefaciens in leaves was completely suppressed by 300 and 500 mg L−1 timentin. The percentage of infected leaves that formed callus ranged from 40 to 48%. In summary, these results demonstrated that timentin was the least toxic among the antibiotics tested, and it is recommended that it be used in Agrobacterium tumefacien-mediated transformation of A. andraeanum.
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The authors are grateful for the financial support received from the Natural Science Foundation of Tangshan Normal College (Grant No. 09A01).
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Zhang, H., Wang, G., Qiao, Y. et al. Effects of timentin and other β-lactam antibiotics on callus induction, shoot regeneration, and rooting in Anthurium andraeanum Linden ex Andre. In Vitro Cell.Dev.Biol.-Plant 53, 219–225 (2017). https://doi.org/10.1007/s11627-017-9823-8
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DOI: https://doi.org/10.1007/s11627-017-9823-8