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In vitro technology at the US Potato Genebank

In Vitro Cellular & Developmental Biology - Plant volume 52, pages 213–225 (2016)Cite this article

Abstract

The US Potato Genebank in Sturgeon Bay, Wisconsin, is the national germplasm collection for the world’s most important vegetable crop. It contains about 6,000 accessions of 100 species of tuber-bearing relatives of Solanum tuberosum. The potato of commerce is a clonal crop susceptible to many systemic pathogens, so the genebank routinely uses in vitro clonal maintenance and distribution for named cultivars. In vitro management is also the tool of choice for various breeding and genetic stocks of interest to breeders and researchers. Long-term backup cryopreservation of clones is done at the base collection in Fort Collins, Colorado. In vitro techniques also play an important role in virus elimination from clones. Recently, in vitro propagation has expanded to the temporary safekeeping of meristem propagules in antimicrobial medium during plant collecting expeditions in the southwest USA. The genebank’s mission includes promoting technology that supports expanded use of the germplasm, in particular, finding ways to overcome interspecific hybridization barriers. Thus, in vitro techniques such as pollen viability testing, ploidy manipulation, protoplast fusion, and embryo rescue have contributed technology for major advances in interspecific hybridization, utilization of noncommercial species, and introgression of genes from wild near-relatives. Finally, advancing in vitro technology holds promise as a tool for mass bioassay and selection of seeds, pollen, or somaclones for useful traits.

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References

  • Albiski F, Alkabani N, Najla S, Sanoubar R, Murshed R (2012) In vitro screening of potato lines for drought tolerance. Physiol Mol Biol Plants 2012:1–7

    Google Scholar 

  • Almekinders CJM, Chujoy E, Thiele G (2009) The use of true potato seed as pro-poor technology: the efforts of an international agricultural research institute to innovating potato production. Potato Res 52:275–293

    Article  Google Scholar 

  • Asakavičiūtė R, Clement C, Ražukas A (2007) The genetic aspect in anther culture of Lithuanian potato (Solanum tuberosum L.) cultivars. Biologija 18:19–22

    Google Scholar 

  • Austin S, Pohlman JD, Brown CR, Mojtahedi H, Santo GS, Douches DS, Helgeson JP (1993) Interspecific somatic hybridization between Solanum tuberosum L. and S. bulbocastanum Dun. as a means of transferring nematode resistance. Am Potato J 70:485–495

    Article  Google Scholar 

  • Aziz AN, Seabrook JEA, Tai GCC, De Jong H (1999) Screening diploid Solanum genotypes responsive to different anther culture conditions and ploidy assessment of anther-derived roots and plantlets. Am J Potato Res 76:9–16

    Article  Google Scholar 

  • Bajaj YPS (1977) Initiation of shoots and callus from potato-tuber sprouts and axillary buds frozen at −196°C. Crop Improv 4:48–53

    Google Scholar 

  • Bamberg JB, del Rio AH (2005) Conservation of genetic resources. In: Razdan MK, Mattoo AK (eds) Genetic improvement of solanaceous crops, vol 1, Potato. Science Publishers, Inc, Enfield

    Google Scholar 

  • Bamberg JB, del Rio AH, Moreyra R (2009) Genetic consequences of clonal versus seed sampling in model populations of two wild potato species indigenous to the USA. Am J Potato Res 86:367–372

  • Bamberg JB, Hanneman RE Jr (1991a) An effective method for culturing pollen tubes of potato. Am Potato J 68:373–379

    Article  Google Scholar 

  • Bamberg JB, Hanneman RE Jr (1991b) Rapid ploidy screening of tuber-bearing Solanum (potato) species through pollen diameter measurement. Am Potato J 68:279–285

    Article  Google Scholar 

  • Batty NP, Dunwell JM (1987) Anther culture of dihaploid Solanum tuberosum H3703. In: Jellis GJ, Richardson DE (eds) The production of new potato varieties: technological advances. Cambridge University Press, Cambridge, pp 289–292

    Chapter  Google Scholar 

  • Bever JD, Felber F (1992) The theoretical population genetics of autopolyploidy. In: Futuyma D, Antonovics J (eds) Oxford surveys in evolutionary biology, vol 8. Oxford University Press, New York, pp 185–218

    Google Scholar 

  • Bolik M, Foroughi-Wehr B, Köhler F, Schuchmann R, Wenzel G (1986) In vitro selection for disease resistance in potato and barley. In: Nuclear techniques and in vitro culture for plant improvement. Proceedings of a symposium jointly organized by the International Atomic Energy Agency and the Food and Agriculture Organization of the United Nations, 19–23 August 1985, Vienna, pp 275–285

  • Boluarte-Medina T, Veilleux RE (2002) Phenotypic characterization and bulk segregant analysis of anther culture response in two backcross families of diploid potato. RAPD markers for androgenesis in potato. Plant Cell Tissue Organ Cult 68:277–286

    Article  CAS  Google Scholar 

  • Brown CR, Mojtahedi H, Zhang LH, Riga E (2009) Independent resistant reactions expressed in root and tuber of potato breeding lines with introgressed resistance to Meloidogyne chitwoodi. Phytopathology 99:1085–1089

  • Brown CR, Yang CP, Mojtahedi H, Santo GS, Masuelli R (1996) RFLP analysis of resistance to Columbia root-knot nematode derived from Solanum bulbocastanum in a BC2 population. Theor Appl Genet 95:572–576

    Article  Google Scholar 

  • Brown CR, Zhang L, Mojtahedi H (2014) Tracking the RMc1 gene for resistance to race 1 of Columbia root-knot nematode (Meloidogyne chitwoodi) in three Mexican wild potato species with different ploidies. Am J Potato Res 91:180–185

    Article  Google Scholar 

  • Butenko RG, Kuchko AA (1980) Somatic hybridization of Solanum tuberosum L. and Solanum chacoense Bitt. by protoplast fusion. In: Fernczy L, Farkas G (eds) Advances in protoplast research. Pergamon Press, Oxford, pp 293–300

    Google Scholar 

  • Butler CD, Gonzalez B, Manjunath KL, Lee RF, Novy RG, Miller JC, Trumble JT (2011) Behavioral responses of adult potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae), to potato germplasm and transmission of Candidatus Liberibacter psyllaurous. Crop Prot 30:1233–1238

    Article  Google Scholar 

  • Cappadocia M, Cheng D, Ludlum-Simonette R (1984) Plant regeneration from in vitro culture of anthers of Solanum chacoense Bitt. and interspecific diploid hybrids S. tuberosum L. × S. chacoense Bitt. Theor Appl Genet 69:139–143

    CAS  PubMed  Google Scholar 

  • Cappadocia M, Cheng DSK, Ludlum-Simonette R (1986) Self-compatibility in doubled haploids and their F1 hybrids, regenerated via anther culture in self-incompatible Solanum chacoense Bitt. Theor Appl Genet 72:66–69

    Article  CAS  PubMed  Google Scholar 

  • Carson GP, Howard HW (1942) Self-incompatibility in certain diploid potato species. Nature 150:290

    Article  Google Scholar 

  • Cho KS, Park TH (2014) Potato breeding via protoplast fusion. J Plant Biotechnol 41:65–72 (in Korean with English abstract)

    Article  Google Scholar 

  • Cipar M, Peloquin S, Hougas R (1967) Haploidy and the identification of self-incompatibility alleles in cultivated potato groups. Can J Genet Cytol 9:511–518

  • Cipar MS, Peloquin SJ, Hougas RW (1964) Variability in the expression of self-incompatibility in tuber-bearing diploid Solanum species. Am Potato J 41:155–162

    Article  Google Scholar 

  • Cristinzio G, Testa A (1999) In vitro evaluation of resistance of potato cultivars to Phytophthora infestans. Potato Res 42:101–105

    Article  Google Scholar 

  • Diaz-Montano J, Vindiola BG, Drew N, Novy RG, Miller JC, Trumble JT (2014) Resistance of selected potato genotypes to the potato psyllid (Hemiptera: Triozidae). Am J Potato Res 91:363–367

    Article  CAS  Google Scholar 

  • Dorrance AE, Inglis DA, Helgeson JP, Brown CR (2001) Partial resistance to Phytophthora infestans in four Solanum crosses. Am J Potato Res 78:9–17

    Article  Google Scholar 

  • Duangpan S, Zhang W, Wu Y, Jansky SH, Jiang J (2013) Insertional mutagenesis using Tnt1 retrotransposon in potato. Plant Physiol 163:21–29

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Eeckhaut T, Lakshmanan PS, Deryckere D, Bockstaele EV, Huylenbroeck JV (2013) Progress in plant protoplast research. Planta 238:991–1003

    Article  CAS  PubMed  Google Scholar 

  • Ensminger ME, Ensminger EH (1993) Foods & nutrition encyclopedia, vol 1, 2nd edn. CRC Press, Boca Raton, FL, p 1104

    Google Scholar 

  • Felcher KJ, Coombs JJ, Massa AN, Hansey CN, Hamilton JP, Veilleux RE, Buell CR, Douches DS (2012) Integration of two diploid potato linkage maps with the potato genome sequence. PLoS One 7:e36347

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ferreira DI, Zelcer A (1989) Advances in protoplast research on Solanum. Int Rev Cytol 115:1–65

    Article  Google Scholar 

  • Ferrie AMR, Caswell KL (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Cult 104:301–309

    Article  Google Scholar 

  • Gillen AM, Novy RG (2007) Molecular characterization of the progeny of Solanum etuberosum identifies a genomic region associated with resistance to potato leafroll virus. Euphytica 155:403–415

    Article  CAS  Google Scholar 

  • Golmirzaie AM, Malagamba P, Pallais N (1994) Breeding potatoes based on true seed propagation. In: Bradshaw JE, Mackay GR (eds) Potato genetics. CAB International, Wallingford, pp 499–513

    Google Scholar 

  • Grout BWW, Henshaw GG (1978) Freeze preservation of potato shoot-tip culture. Ann Bot 42:1227–1229

    Google Scholar 

  • Grout BWW, Henshaw GG (1980) Structural observations on the growth of potato shoot-tip culture after thawing from liquid nitrogen. Ann Bot 46:243–248

    Google Scholar 

  • Habe I, Ohbayashi K (2014) In vitro screening assay of resistance to bacterial wilt caused by Ralstonia solanacearum in potato with sterile cultured plants. Kyushu Plant Prot Res 60:1–8

    Article  Google Scholar 

  • Hackett CA, McLean K, Bryan GJ (2013) Linkage analysis and QTL mapping using SNP dosage data in a tetraploid potato mapping population. PLoS One 8:e63939

    Article  PubMed  PubMed Central  Google Scholar 

  • Haldane JB (1930) Theoretical genetics of autopolyploids. J Genet 22:359–372

    Article  Google Scholar 

  • Halterman D, Kramer LC, Weilgus S, Jiang J (2008) Performance of transgenic potato containing the late blight resistance gene RB. Plant Dis 92:339–343

    Article  CAS  Google Scholar 

  • Hawkes JG (1990) The potato: evolution, biodiversity and genetic resources. Belhaven Press, London

    Google Scholar 

  • Helgeson JP, Pohlman JD, Austin S, Haberlach GT, Wielgus SM, Ronis D, Zambolim L, Tooley P, McGrath JM, James RV (1998) Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor Appl Genet 96:738–742

    Article  Google Scholar 

  • Hermsen JGT, Verdenius J (1973) Selection from Solanum tuberosum Group Phureja of genotypes combining high-frequency haploid induction with homozygosity for embryo-spot. Euphytica 22:244–259

    Article  Google Scholar 

  • Hougas R, Peloquin S, Gabert A (1964) Effect of seed-parent and pollinator on frequency of haploids in Solanum tuberosum. Crop Sci 4:593–595

    Article  Google Scholar 

  • Hudák I, Hevesi M, Dobránszki J, Magyar-Tábori K (2009) In vitro tests of resistance to soft rot Erwiniae on potato tubers. Acta Horticult 812:103–106

    Article  Google Scholar 

  • Jansky SH, Chung YS, Kittipadukal P (2014) M6: a diploid potato inbred line for use in breeding and genetics research. J Plant Regul 8:195–199

    Article  Google Scholar 

  • Johansson LB (1988) Increased induction of embryogenesis and regeneration in anther cultures of Solanum tuberosum L. Potato Res 31:145–149

    Article  Google Scholar 

  • Johnston SA, den Nijs TPM, Peloquin SJ, Hanneman RE Jr (1980) The significance of genic balance to endosperm development in interspecific crosses. Theor Appl Genet 57:5–9

    Article  CAS  PubMed  Google Scholar 

  • Jupe F, Pritchard L, Etherington GJ, MacKenzie K, Cock PJA, Wright F, Sharma SK, Bolser D, Bryan GJ, Jones JDG, Hein I (2012) Identification and localisation of the NB-LRR gene family within the potato genome. BMC Genomics 13:75

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kaczmarczyk A, Rokka V-M, Keller ERJ (2011) Potato shoot tip cryopreservation. A review. Potato Res 54:45–79

    Article  Google Scholar 

  • Karp A, Jones MGK, Foulger D, Fish N, Bright SWJ (1989) Variability in potato tissue culture. Am Potato J 66:669–684

  • Karp A, Risiott R, Jones MK, Bright SJ (1984) Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants. Plant Cell Tissue Organ Cult 3:363–373

    Article  Google Scholar 

  • Kelley KB, Whitworth JL, Novy RG (2009) Mapping of the potato leafroll virus resistance gene, Rlr etb , from Solanum etuberosum identifies interchromosomal translocations among its E-genome chromosomes 4 and 9 relative to the A-genome of Solanum L. sect. Petota. Mol Breed 23:489–500

    Article  CAS  Google Scholar 

  • Khan M, Munive S, Bonierbale M (2015) Early generation in vitro assay to identify potato populations and clones tolerant to heat. Plant Cell Tissue Organ Cult 121:45–52

    Article  Google Scholar 

  • Khrais T, Leclerc Y, Donnelly DJ (1998) Relative salinity tolerance of potato cultivars assessed by in vitro screening. Am J Potato Res 75:207–210

    Article  Google Scholar 

  • Khu D, Love SL, Mo H, Li K, Lim H (2007) In vitro screening of potato genotypes for resistance to common scab using thaxtomin A. Hortic Environ Biotechnol 48:224–227

    CAS  Google Scholar 

  • Kim HH, Yoon JW, Park YE, Cho EG, Sohn JK, Kim TS, Engelman F (2006) Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification. Cryo-Letters 27:223–234

    CAS  PubMed  Google Scholar 

  • Kitazumi A, Kawahara Y, Onda TS, De Koeyer D, de los Reyes BG, Cloutier S (2015) Implications of miR166 and miR159 induction to the basal response mechanisms of an andigena potato (Solanum tuberosum subsp. andigena) to salinity stress, predicted from network models in Arabidopsis. Genome 58:13–24

    Article  CAS  PubMed  Google Scholar 

  • Klein RE, Livingston CH (1982) Eradication of potato virus X from potato by ribavirin treatment of cultured potato shoot tips. Am Potato J 59:359–365

    Article  CAS  Google Scholar 

  • Kloosterman B, Abelenda JA, Gomez MMC, Oortwijn M, de Boer JM, Kowitwanich K, Horvath BM, van Eck HJ, Smaczniak C, Prat S, Visser RGF, Bachem CWB (2013) Naturally occurring allele diversity allows potato cultivation in northern latitudes. Nature 495:246–250

    Article  CAS  PubMed  Google Scholar 

  • Kotch GP, Peloquin SJ (1987) A new source of haploid germplasm for genetic and breeding research. Am Potato J 64:137–141

    Article  Google Scholar 

  • Lakhotia N, Joshi G, Bhardwaj AR, Katiyar-Agarwal S, Agarwal M, Jagannath A, Goel S, Kumar A (2014) Identification and characterization of miRNAome in root, stem, leaf and tuber developmental stages of potato (Solanum tuberosum L.) by high-throughput sequencing. BMC Plant Biol 14:6

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214

    Article  CAS  PubMed  Google Scholar 

  • Lightbourn G, Veilleux R (2007) Production and evaluation of somatic hybrids derived from monoploid potato. Am J Potato Res 84:425–435

    Article  Google Scholar 

  • Lindhout P, Meijer D, Schotte T, Hutten RCB, Visser RGF, van Eck HJ (2011) Towards F1 hybrid seed potato breeding. Potato Res 54:301–312

    Article  Google Scholar 

  • Liu CA, Douches DS (1993) Production of haploids of potato (Solanum tuberosum subsp. tuberosum) and their identification with electrophoretic analysis. Euphytica 70:113–126

    Article  Google Scholar 

  • Lozano R, Ponce O, Ramirez M, Mostajo N, Orjeda G (2012) Genome-wide identification and mapping of NBS-encoding resistance genes in Solanum tuberosum group phureja. PLoS One 7:e34775

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • M’Ribu HK, Veilleux R (1992) Fertility of doubled monoploids of Solanum phureja. Am Potato J 69:447–459

    Article  Google Scholar 

  • Macaso-Khwaja AC, Peloquin SJ (1983) Tuber yields of families from open pollinated and hybrid true potato seed. Am Potato J 60:645–651

    Article  Google Scholar 

  • Machida-Hirano R (2015) Diversity of potato genetic resources. Breed Sci 65:26–40

    Article  PubMed  PubMed Central  Google Scholar 

  • Marks GE (1966) The origin and significance of intraspecific polyploidy: experimental evidence from Solanum chacoense. Evolution 20:552–557

    Article  Google Scholar 

  • Meier A, Jansky S, Halterman D (2015) Germplasm release: three potato clones incorporating combined resistances to early blight from S. palustre and late blight from S. bulbocastanum into a S. tuberosum background. Am J Potato Res 92:410–416

    Article  CAS  Google Scholar 

  • Melchers G, Sacristan MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsb Res Commun 43:203–218

    Article  Google Scholar 

  • Mendiburu AO, Peloquin SJ (1977) The significance of 2N gametes in potato breeding. Theor Appl Genet 49:53–61

    Article  CAS  PubMed  Google Scholar 

  • Meyer R, Salamini F, Uhrig H (1993) Isolation and characterization of potato diploid clones generating a high frequency of monohaploid or homozygous diploid androgenetic plants. Theor Appl Genet 85:905–912

    CAS  PubMed  Google Scholar 

  • Mojtahedi H, Brown CR, Santo GS (1995) Characterization of resistance in a somatic hybrid of Solanum bulbocastanum and S. tuberosum to Meloidogyne chitwoodi. J Nematol 27:86–93

    CAS  PubMed  PubMed Central  Google Scholar 

  • M’Ribu KH, Veilleux RE (1990) Effect of genotype, explant, subculture interval and environmental conditions on regeneration of shoots from in vitro monoploids of a diploid potato species, Solanum phureja Juz. & Buk. Plant Cell Tissue Organ Cult 23:171–179

    Google Scholar 

  • Muñiz García M, Giammaria V, Grandellis C, Téllez-Iñón M, Ulloa R, Capiati D (2012) Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro. Planta 235:761–778

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2000) Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet 101:697–704

    Article  CAS  Google Scholar 

  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2001) Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines. Mol Genet Genomics 265:694–704

    Article  CAS  PubMed  Google Scholar 

  • Nassar AMK, Kubow S, Donnelly DJ (2015) Somatic embryogenesis for potato (Solanum tuberosum L.) improvement. Chapter 8. In: Li XQ, Donnelly DJ, Jensen TG (eds) Somatic genome manipulation: advances, methods and applications. Springer-Verlag, New York, 375

    Google Scholar 

  • Novy RG, Gillen AM, Whitworth JL (2007) Characterization of the expression and inheritance of potato leafroll virus (PLRV) and potato virus Y (PVY) resistance in three generations of germplasm derived from Solanum etuberosum. Theor Appl Genet 114:1161–1172

  • Novy RG, Helgeson JP (1994a) Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones. Theor Appl Genet 89:775–782

    CAS  PubMed  Google Scholar 

  • Novy RG, Helgeson JP (1994b) Resistance to potato virus Y in somatic hybrids between Solanum etuberosum and S. tuberosum × S. berthaultii hybrid. Theor Appl Genet 89:783–786

    CAS  PubMed  Google Scholar 

  • Novy RG, Nasruddin A, Ragsdale DW, Radcliffe EB (2002) Genetic resistances to potato leafroll virus, potato virus Y, and green peach aphid in progeny of Solanum etuberosum. Am J Potato Res 79:9–18

    Article  Google Scholar 

  • Obidiegwu J, Flath K, Gebhardt C (2014) Managing potato wart: a review of present research status and future perspective. Theor Appl Genet 127:763–780

    Article  PubMed  PubMed Central  Google Scholar 

  • Ortiz R (2001) The state of the use of potato genetic diversity. In: Cooper HD, Spillane C, Hodgkin T (eds) Broadening the genetic base of crop production. CABI Publishing, Wallingford, pp 181–200

    Chapter  Google Scholar 

  • Ortiz R, Ehlenfeldt MK (1992) The importance of Endosperm Balance Number in potato breeding and the evolution of tuber-bearing Solanum species. Euphytica 60:105–113

    Google Scholar 

  • Pandey SK, Sarkar D, Sharma S, Chandel P (2010) Integration of somatic fusion into potato breeding: problems and perspectives. Am Potato J 37:9–20

    Google Scholar 

  • Paz MM, Veilleux RE (1999) Influence of culture medium and in vitro conditions on shoot regeneration in Solanum phureja monoploids and fertility of regenerated doubled monoploids. Plant Breed 118:53–57

    Article  CAS  Google Scholar 

  • Phumichai C, Mori M, Kobayashi A, Kamijima O, Hosaka K (2005) Toward the development of highly homozygous diploid potato lines using the self-compatibility controlling Sli gene. Genome 48:977–984

    Article  CAS  PubMed  Google Scholar 

  • Potato Genome Sequencing Consortium (2011) Genome sequence and analysis of the tuber crop potato. Nature 475:189–195

    Article  CAS  Google Scholar 

  • Powell W, Uhrig H (1987) Anther culture of Solanum genotypes. Plant Cell Tissue Organ Cult 11:13–24

    Article  Google Scholar 

  • Rao PS, Suprasanna P (1996) Methods to double haploid chromosome numbers. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 1. Springer Netherlands, Dordrecht, pp 317–339

    Chapter  Google Scholar 

  • Říhová L, Hrabětová E, Tupý J (1996) Optimization of conditions for in vitro pollen germination and tube growth in potatoes. Int J Plant Sci 157:561–566

    Article  Google Scholar 

  • Rodríguez NV, Kowalski B, Rodríguez LG, Caraballoso IB, Suárez MA, Pérez PO, Quintana CR, González N, Ramos RQ (2007) In vitro and ex vitro selection of potato plantlets for resistance to early blight. J Phytopathol 155:582–586

    Article  Google Scholar 

  • Rokka VM (2015) Protoplast technology in genome manipulation of potato through somatic cell fusion. In: Li X, Donnelly DJ, Jensen TG (eds) Somatic genome manipulation. Springer, New York, pp 217–235

    Chapter  Google Scholar 

  • Santra M, Ankrah N, Santra DK, Kidwell KK (2012) An improved wheat microspore culture technique for the production of doubled haploid plants. Crop Sci 52:2314–2320

    Article  Google Scholar 

  • Sharma SK, Bolser D, de Boer J, Sonderkaer M, Amoros W, Carboni MF, D’Ambrosio JM, de la Cruz G, Di Genova A, Douches DS, Eguiluz M, Guo X, Guzman F, Hackett CA, Hamilton JP, Li GC, Li Y, Lozano R, Maass A, Marshall D, Martinez D, McLean K, Mejia N, Milne L, Munive S, Nagy I, Ponce O, Ramirez M, Simon R, Thomson SJ, Torres Y, Waugh R, Zhang ZH, Huang SW, Visser RGF, Bachem CWB, Sagredo B, Feingold SE, Orjeda G, Veilleux RE, Bonierbale M, Jacobs JME, Milbourne D, Martin DMA, Bryan GJ (2013) Construction of reference chromosome-scale pseudomolecules for potato: integrating the potato genome with genetic and physical maps. G3 (Bethesda) 3:2031–2047

    Article  PubMed Central  CAS  Google Scholar 

  • Snider KT, Veilleux RE (1994) Factors affecting variability in anther culture and in conversion of androgenic embryos of Solanum phureja. Plant Cell Tissue Organ Cult 36:345–354

    Article  Google Scholar 

  • Song J, Bradeen JM, Naess SK, Raasch JA, Wielgus SM, Haberlach GT, Liu J, Kuang H, Austin-Phillips S, Buell CR, Helgeson JP, Jiang J (2003) Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proc Natl Acad Sci U S A 100:9128–9133

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson AL, Taylor RJ, Pasche JS, Novy RG, Gudmestad NC (2007) Resistance to Phytophthora erythroseptica and Pythium ultimum in a potato clone derived from S. berthaultii and S. etuberosum. Am J Potato Res 84:149–160

    Article  Google Scholar 

  • Towill LE (1981) Survival at low temperatures of shoot-tips from cultivars of Solanum tuberosum Group Tuberosum. Cryo-Letters 2:373–382

    Google Scholar 

  • Towill LE (1983) Improved survival after cryogenic exposure of shoot tips derived from in vitro plantlet cultures of potato. Cryobiology 20:567–573

    Article  CAS  PubMed  Google Scholar 

  • Towill LE (1984) Survival at ultra-low temperature of shoot tips from Solanum tuberosum groups Andigena, Phureja, Stenotomum, Tuberosum and other tuber-bearing Solanum species. Cryo-Letters 5:319–326

    Google Scholar 

  • Uijtewaal BA, Huigen DJ, Hermsen JGT (1987) Production of potato monohaploids (2n = x = 12) through prickle pollination. Theor Appl Genet 73:751–758

    Article  CAS  PubMed  Google Scholar 

  • Upadhya M, Cabello R (1997) Interaction of maternal clones of potato (Solanum tuberosum) with pollinator clones of S. phureja for induced parthenogenesis. Impact on a changing world Program Report 1997–98. International Potato Center, Lima, pp 179–184

    Google Scholar 

  • van der Vossen E, Sikkema A, Hekkert BL, Gros J, Stevens P, Muskens M, Wouters D, Pereira A, Stiekema W, Allefs S (2003) An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. Plant J 36:867–882

    Article  PubMed  CAS  Google Scholar 

  • Vega SE, Bamberg JB, Palta JP (2006) Gibberellin-deficient dwarfs in potato vary in exogenous GA3 response when the ga1 allele is in different genetic backgrounds. Am J Potato Res 83:357–363

    Article  CAS  Google Scholar 

  • Veilleux R (1985) Diploid and polyploid gametes in crop plants: mechanisms of formation and utilization in plant breeding. Plant Breed Rev 3:253–288

    Google Scholar 

  • Veilleux RE (1990) Solanum phureja: anther culture and the induction of haploids in a cultivated diploid potato species. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 12: haploids in crop improvement I. Springer-Verlag, New York, pp 530–543

    Chapter  Google Scholar 

  • Veilleux RE, Johnson AAT (1998) Somaclonal variation: molecular analysis, transformation interaction, and utilization. Plant Breed Rev 16:229–268

    CAS  Google Scholar 

  • Wenzel G, Schieder O, Przewozny T, Sopory SK, Melchers G (1979) Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theor Appl Genet 55:49–55

    Article  CAS  PubMed  Google Scholar 

  • Zaki H, Haynes K, Vinyard B (2016) In vitro screening of Solanum chacoense for salinity tolerance. Am J Potato Res 93(abstract)

  • Zhang N, Liu B, Ma C, Zhang G, Chang J, Si H, Wang D (2014) Transcriptome characterization and sequencing-based identification of drought-responsive genes in potato. Mol Biol Rep 41:505–517

  • Zhang R, Marshall D, Bryan GJ, Hornyik C (2013) Identification and characterization of miRNA transcriptome in potato by high-throughput sequencing. PLoS One 8:e57233

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI, 54235, USA

    J. B. Bamberg & M. W. Martin

  2. USDA-APHIS-PPQ-FO-Plant Germplasm Quarantine Program (PGQP), Beltsville, MD, 20705, USA

    J. Abad

  3. National Center for Genetic Resources Preservation, USDA-ARS, 1111 Mason Street, Fort Collins, CO, 80521, USA

    M. M. Jenderek & J. Tanner

  4. Plant Science Department, McGill University, 21,111 Lakeshore Rd., Ste Anne de Bellevue, QC, H9X 3V9, Canada

    D. J. Donnelly

  5. Plant Protection Department, Faculty of Agriculture, Damanhour University, Damanhour, Albeheira, 22516, Egypt

    A M. K. Nassar

  6. Department of Horticulture, Virginia Tech, Blacksburg, VA, 24061, USA

    R. E. Veilleux

  7. Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID, 83210, USA

    R. G. Novy

Authors
  1. J. B. Bamberg
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  2. M. W. Martin
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  3. J. Abad
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  4. M. M. Jenderek
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  5. J. Tanner
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  6. D. J. Donnelly
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  7. A M. K. Nassar
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  8. R. E. Veilleux
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  9. R. G. Novy
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Corresponding author

Correspondence to J. B. Bamberg.

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Editor: David Duncan

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Bamberg, J.B., Martin, M.W., Abad, J. et al. In vitro technology at the US Potato Genebank. In Vitro Cell.Dev.Biol.-Plant 52, 213–225 (2016). https://doi.org/10.1007/s11627-016-9753-x

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  • DOI: https://doi.org/10.1007/s11627-016-9753-x

Keywords

  • Potato
  • Solanum
  • Genebank
  • Germplasm collection
  • In vitro conservation