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Evaluation of aqueous chlorine dioxide for disinfecting plant explants

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Abstract

Sterilization of explants while maintaining viability is a major task for those working in plant tissue culture. This task is usually accomplished through the use of mercuric chloride or sodium hypochlorite. Due to environmental and health concerns, however, new disinfectants are desired. Chlorine dioxide is an environmentally friendly disinfectant that has been used for many purposes, though its use in plant tissue culture has not been thoroughly investigated. This report describes the application of aqueous chlorine dioxide for the disinfection of yacon (Smallanthus sonchifolius) and pomegranate (Punica granatum) explants. The threshold concentration for obtaining high antimicrobial efficiency with aqueous chlorine dioxide was determined to be 1.25–2.5 mM; however, the viability of the explants from the two species differed with treatment. While the viability of yacon explants remained high at 1.25–1.67 mM aqueous chlorine dioxide, pomegranate explant viability was severely impaired at all tested concentrations. The level of explant viability was negatively correlated with high levels of endogenous polyphenol and subsequent free radical accumulation following treatment. Aqueous chlorine dioxide at concentrations of 1.25–2.5 mM was also suitable for explant disinfection in other low-polyphenol-containing plant species such as rice (Oryza sativa), Pinellia ternata, and Isodon amethystoides. This work shows that aqueous chlorine dioxide can be effectively utilized as an alternative disinfectant for low-polyphenol-containing explants in general.

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References

  • Bhawana, Stubblefield JM, Newsome AL, Cahoon AB (2015) Surface decontamination of plant tissue explants with chlorine dioxide gas. In Vitro Cell Dev Biol Plant 51:214–219

    Article  CAS  Google Scholar 

  • Budavari S, O’Neil MJ, Smith A, Heckelman PE, Kinneary JF (2001) The Merck index: an encyclopedia of chemicals, drugs and biologicals, 13th edn. Merck Research Laboratories, Whitehouse Station, p 362

    Google Scholar 

  • Coqueiro A, Regasini LO, Skrzek SCG, Queiroz MMF, Silva DHS, da Silva BV (2013) Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae). Molecules 18:2376–2385

    Article  PubMed  CAS  Google Scholar 

  • de Santana JRF, Paiva R, Aloufa MAI, de Lemos EEP (2003) Efficiency of ampicillin and benomyl at controlling contamination of Annonaceae leaf segments cultured in vitro. Fruits 58:357–361

    Article  Google Scholar 

  • Devi CS, Srinivasan VM (2006) Studies on various atmospheric microorganisms affecting the plant tissue culture explants. Am J Plant Physiol 1:205–209

    Article  Google Scholar 

  • Erhardt AJ, Rezende CE, Walker BG, Franceschi D, Downie D (2015) Mercury concentrations and awareness in Campos dos Goytacazes, Brazil: baseline measures for examining the efficacy of the Minamata Convention. J Environ Stud Sci 5:517–525

    Article  Google Scholar 

  • Fu X, Zhou HF, Li C, Zhao ZG, Yu SJ (2014) Distribution and antioxidant activity of free and bound phenolic acids in sugarcane tissues. Mod Food Sci Technol 30:17–22

    CAS  Google Scholar 

  • Hsu CS, Huang DJ (2013) Disinfection efficiency of chlorine dioxide gas in student cafeterias in Taiwan. J Air Waste Manage Assoc 63:796–805

    Article  CAS  Google Scholar 

  • Hsu CS, Lu MC, Huang DJ (2015) Disinfection of indoor air microorganisms in stack room of university library using gaseous chlorine dioxide. Environ Monit Assess 187:34

    Article  PubMed  CAS  Google Scholar 

  • Jee SH, Kim DG, Jeong K, Ko SO (2013) Reduction of disinfection byproduct formation by an oxidative coupling reaction with birnessite. KSCE J Civ Eng 17:1241–1250

    Article  Google Scholar 

  • Jova MC, González JE (2014) Ozone as an alternative for disinfection of explants during in vitro mass plant propagation. Ozone Sci Eng 36:435–439

    Article  CAS  Google Scholar 

  • Latoui M, Aliakbarian B, Casazza AA, Seffen M, Converti A, Perego P (2012) Extraction of phenolic compounds from Vitex agnus-castus L. Food Bioprod Process 90:748–754

    Article  CAS  Google Scholar 

  • Li Q, Zhang MW, Zhang RF, Wei ZC, Deng YY, Tang XJ, Zhang YH, Wu L, Ma YX (2012) Free and bound phenolic contents and antioxidant activity of five varieties of indica rice husk. Agric Sci China 45:1150–1158

    CAS  Google Scholar 

  • Lin T, Chen W, Cai B (2014) The use of chlorine dioxide for the inactivation of copepod zooplankton in drinking water treatment. Environ Technol 35:2846–2851

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Ni Y, Shen X, van Heiningen ARP (1994) Studies on the reactions of phenolic and non-phenolic lignin model compounds with chlorine dioxides. J Wood Chem Technol 14:243–262

    Article  CAS  Google Scholar 

  • Park SH, Kang DH (2015) Antimicrobial effect of chlorine dioxide gas against foodborne pathogens under differing conditions of relative humidity. LWT–Food. Sci Technol 60:186–191

    CAS  Google Scholar 

  • Rajmohan K, Soni KB, Swapna A, Nazeem PA, Shimi SS (2010) Use of copper sulphate for controlling systemic contamination in black pepper (Piper nigrum L.) cultures. J Food Agric Environ 8:569–571

    CAS  Google Scholar 

  • Rifaki N, Economou AS, Hatzilazarou S, Hatzistathis A (2009) Shoot regeneration from shoot tip explants of Juniperus excelsa Bieb. Propag Ornam Plants 9:53–55

    Google Scholar 

  • Sathyanarayana BN, Varghese DB (2007) Plant tissue culture: practices and new experimental protocols. IK International Publishing House, New Delhi, pp 1–8

    Google Scholar 

  • Simon FX, Berdalet E, Gracia FA, España F, Llorens J (2014) Seawater disinfection by chlorine dioxide and sodium hypochlorite: a comparison of biofilm formation. Water Air Soil Pollut 225:1921

    Article  CAS  Google Scholar 

  • Teixeira da Silva JA (2014) Organogenesis from chrysanthemum (Dendranthema × grandiflora (Ramat.) Kitamura) petals (disc and ray florets) induced by plant growth regulators. Asia Pac J Mol Biol Biotechnol 22:145–151

    Google Scholar 

  • Trinetta V, Linton RH, Morgan MT (2013) The application of high-concentration short-time chlorine dioxide treatment for selected specialty crops including Roma tomatoes (Lycopersicon esculentum), cantaloupes (Cucumis melo ssp. melo var. cantaloupensis) and strawberries (Fragaria × ananassa). Food Microbiol 34:296–302

    Article  PubMed  CAS  Google Scholar 

  • Wu B, Guo Q, Wang GX, Peng XY, Wang JD, Che FB (2014) Effects of different postharvest treatments on the physiology and quality of ‘Xiaobai’ apricots at room temperature. J Food Sci Technol 52:2247–2255

    Article  PubMed  CAS  Google Scholar 

  • Wu VC, Rioux A (2010) A simple instrument-free gaseous chlorine dioxide method for microbial decontamination of potatoes during storage. Food Microbiol 27:179–184

    Article  PubMed  CAS  Google Scholar 

  • Yildiz M, Ekiz H (2014) The effect of sodium hypochlorite solutions on in vitro seedling growth and regeneration capacity of sainfoin (Onobrychis viciifolia Scop.) hypocotyl explants. Can J Plant Sci 94:1161–1164

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31501368, 81573518), the Key Project of Natural Science Research of Universities in Anhui Province, China (KJ2014A226, KJ2015ZD35), and the Natural Science Foundation of Anhui Province, China (1408085MC58, 1608085MC52).

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Authors and Affiliations

  1. Key Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal University, Huaibei, 235000, China

    Yongbo Duan, Fenglan Zhao, Huan Li, Yingying Zhou, Xiuyun Zhu & Jianping Xue

  2. Beijing Bijieshi Environment-Friendly Technique Development Co., Ltd., Beijing, 101102, China

    Fenglan Li

  3. Institute of Plant and Environmental Protection, Beijing Academy of Agricultural and Forestry Science, Beijing, 100097, China

    Wenliang Chen

Authors
  1. Yongbo Duan
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  2. Fenglan Zhao
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  3. Huan Li
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  4. Yingying Zhou
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  5. Xiuyun Zhu
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  6. Fenglan Li
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  7. Wenliang Chen
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  8. Jianping Xue
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Correspondence to Jianping Xue.

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Editor: Baochun Li

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Duan, Y., Zhao, F., Li, H. et al. Evaluation of aqueous chlorine dioxide for disinfecting plant explants. In Vitro Cell.Dev.Biol.-Plant 52, 38–44 (2016). https://doi.org/10.1007/s11627-015-9736-3

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  • DOI: https://doi.org/10.1007/s11627-015-9736-3

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